CAJ | 학술논문

目的探讨广金钱草水提取液对肾草酸钙结石模型大鼠氧化应激的影响. 方法采用乙二醇饮水和氯化铵灌胃法诱导建立大鼠肾草酸钙结石模型,将50只8周龄Wistar健康雄性大鼠按完全随机设计法随机分为5组(n=10):空白组(A组)、模型组(B组)、广金钱草低剂量组(C组)、广金钱草中剂量组(D组)和广金钱草高剂量组(E组),其中A组采用自来水饮水+生理盐水2 ml/d灌胃,B组采用1％乙二醇饮水+2％氯化铵2 ml/d灌胃,C、D、E组在B组基础上分别采用0.6、1.2、2.4 g/ml广金钱草水提取液2 ml/d灌胃28 d.各组大鼠在相同条件下饲养28 d后收集血液及双肾标本,左肾组织做HE染色石蜡切片,光镜观察草酸钙结晶情况;测定血液和右肾匀浆组织中丙二醛、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)含量等了解氧化应激情况. 结果肾组织草酸钙结晶方面,与A组(0±0)比较,B组(7.1±2.9)结晶评分显著增高(P＜0.01),C组(1.6±1.4)、D组(0.4±0.7)和E组(1.1±1.1)结晶评分较B组显著降低(P＜0.01),其中D组降低最显著.氧化应激指标方面,与A组比较,B组大鼠血、肾组织丙二醛浓度显著升高(P＜0.01),使用广金钱草水提取液(C组、D组、E组)干预可减少血、肾组织丙二醛水平(P＜0.01).与A组比较,B组大鼠血、肾组织SOD、GSH-Px、CAT的浓度显著降低(P＜0.01),使用广金钱草水提取液各剂量组(C组、D组、E组)干预可以显著增加肾匀浆SOD水平(P＜0.01),其中D组尚可显著增加血SOD、GSH-Px的水平(P＜0.05),但广金钱草水提取液各剂量组对血CAT及肾匀浆GSH-Px、CAT无显著影响(P＞0.05). 结论中剂量广金钱草水提取液在肾草酸钙结石模型大鼠中可明显抑制晶体形成,并有一定的抗氧化作用.
목적 탐토엄금전초수제취액대신초산개결석모형대서양화응격적영향. 방법 채용을이순음수화록화안관위법유도건립대서신초산개결석모형,장50지8주령Wistar건강웅성대서안완전수궤설계법수궤분위5조(n=10):공백조(A조)、모형조(B조)、엄금전초저제량조(C조)、엄금전초중제량조(D조)화엄금전초고제량조(E조),기중A조채용자래수음수+생리염수2 ml/d관위,B조채용1％을이순음수+2％록화안2 ml/d관위,C、D、E조재B조기출상분별채용0.6、1.2、2.4 g/ml엄금전초수제취액2 ml/d관위28 d.각조대서재상동조건하사양28 d후수집혈액급쌍신표본,좌신조직주HE염색석사절편,광경관찰초산개결정정황;측정혈액화우신균장조직중병이철、초양화물기화매(SOD)、곡광감태과양화물매(GSH-Px)화과양화경매(CAT)함량등료해양화응격정황. 결과 신조직초산개결정방면,여A조(0±0)비교,B조(7.1±2.9)결정평분현저증고(P＜0.01),C조(1.6±1.4)、D조(0.4±0.7)화E조(1.1±1.1)결정평분교B조현저강저(P＜0.01),기중D조강저최현저.양화응격지표방면,여A조비교,B조대서혈、신조직병이철농도현저승고(P＜0.01),사용엄금전초수제취액(C조、D조、E조)간예가감소혈、신조직병이철수평(P＜0.01).여A조비교,B조대서혈、신조직SOD、GSH-Px、CAT적농도현저강저(P＜0.01),사용엄금전초수제취액각제량조(C조、D조、E조)간예가이현저증가신균장SOD수평(P＜0.01),기중D조상가현저증가혈SOD、GSH-Px적수평(P＜0.05),단엄금전초수제취액각제량조대혈CAT급신균장GSH-Px、CAT무현저영향(P＞0.05). 결론 중제량엄금전초수제취액재신초산개결석모형대서중가명현억제정체형성,병유일정적항양화작용.
Objective To investigate the effects of aqueous extract from Desmodium styracifolium (Osb.) Merr.(Ds) on oxidative stress of renal calcium oxalate (CaOx) rat models.Methods The models of renal CaOx were established with ethylene glycol in drinking water and ammonium chloride by gavage.The 50 adult healthy male Wistar strain rats were randomly divided into 5 groups (10 of each),i.e.,normal control group (group A),calcium oxalate model group (group B),low-dose Ds extract-treated group (group C),moderate-dose Ds extract-treated group (group D) and high-dose Ds extract-treated group (group E).Group A was established with drinking water and physiological saline by gavage (2 ml per day),group B was established with 1％ ethylene glycol in drinking water and 2％ ammonium chloride by gavage (2 ml per day),group C,D and E were established with aqueous extract from Ds (0.6,1.2 and 2.4 g/ml,respectively) by gavage on the basis of group B (2 ml per day).After feeding for 28 days,blood and renal samples were collected.The presence of CaOx crystals was scored in left kidneys under hematoxylin and eosin staining,and the levels of malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and catalase (CAT) in the blood and right kidney homogenate were measured as indicators of oxidative stress.Results For the formation of CaOx crystals,the scores of group B were significantly higher than group A (P＜0.01),the scores of group C,D and E were significantly lower than group B (P＜0.01),especially the group D showed the best effect.For the parameters of oxidative stress,compared with group A,the concentration of MDA in blood and kidney tissues of group B significantly increased (P＜0.01),which significantly decreased (P＜0.01) after the intervention of Ds (group C,D and E).Compared with group A,the content of SOD,CAT and GSH-Px in group B significantly decreased (P＜0.01),the SOD level in renal homogenate significantly increased (P＜0.01) after the intervention of Ds (group C,D and E).In group D,the SOD and GSH-Px levels in blood significantly increased (P＜0.05).But the Ds showed no significant influence either for CAT in blood or GSH-Px and CAT in renal homogenate (P＞0.05).Conclusion The moderate-dose Ds extract seems to prevent the formation of renal CaOx in rats,which is probably due to its antioxidant effects.