CAJ | 학술논문

目的评价碱性成纤维细胞生长因子(FGF-2)在大鼠神经病理性痛中的作用.方法雄性SD大鼠100只,7周龄,体重200 ～ 250 g,采用随机数字表法,将大鼠随机分为4组(n=25):假手术组(S组)、神经病理性痛组(NP组)、PBS组、FGF-2抗体组(Ab组).于术后1、6、9、13、16、20d时Ab组鞘内注射FGF-2抗体18μg(40 μl),PBS组鞘内注射等容量的PBS溶液.采用坐骨神经分支选择性损伤的方法制备神经病理性痛模型.于术前ld,术后1、4、7、14、21 d时观察痛行为学,测定机械缩足反射阈值(PWMT),并于术后各时点应用ELISA法检测脊髓TNF-α和IL-6含量.结果与S组比较,NP组、PBS组和Ab组PWMT降低,脊髓TNF-α和IL-6含量升高(P＜0.05).与NP组和PBS组比较,Ab组PWMT升高,脊髓TNF-α和IL-6含量降低(P＜0.05).结论 FGF-2参与大鼠神经病理性痛的发生和发展,其机制与诱发脊髓组织炎性反应有关.
목적 평개감성성섬유세포생장인자(FGF-2)재대서신경병이성통중적작용.방법 웅성SD대서100지,7주령,체중200 ～ 250 g,채용수궤수자표법,장대서수궤분위4조(n=25):가수술조(S조)、신경병이성통조(NP조)、PBS조、FGF-2항체조(Ab조).우술후1、6、9、13、16、20d시Ab조초내주사FGF-2항체18μg(40 μl),PBS조초내주사등용량적PBS용액.채용좌골신경분지선택성손상적방법제비신경병이성통모형.우술전ld,술후1、4、7、14、21 d시관찰통행위학,측정궤계축족반사역치(PWMT),병우술후각시점응용ELISA법검측척수TNF-α화IL-6함량.결과 여S조비교,NP조、PBS조화Ab조PWMT강저,척수TNF-α화IL-6함량승고(P＜0.05).여NP조화PBS조비교,Ab조PWMT승고,척수TNF-α화IL-6함량강저(P＜0.05).결론 FGF-2삼여대서신경병이성통적발생화발전,기궤제여유발척수조직염성반응유관.
Objective To evaluate the role of fibroblast growth faetor-2 (FGF-2) in neuropathic pain (NP) in rats.Methods One hundred male Sprague-Dawley rats,aged 7 weeks,weighing 200-250 g,were randomly divided into 4 groups (n=25 each): sham operation group (group S),group NP,phosphate buffer solution (PBS) group and FGF-2 antibody group (group Ab).FGF-2 antibody 18 μg (40 μl) was injected intrathecally at 1,6,9,13,16 and 20 days after operation in group Ab,while the equal volume of PBS was injected intrathecally in group PBS.Spinal nerve injury was produced by exposing the sciatic nerve and its branches and ligation and transection of tibial nerve and common fibular nerve.Pain behavior was assessed at 1 day before operation and at 1,4,7,14 and 21 days after operation.The paw withdrawal mechanical threshold (PWMT) was measured.The animals were then sacrificed and the lumbar segment (L4-6) of the spinal cord was obtained for determination of the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the spinal cord.Results Compared with group S,the PWMT was significantly decreased and the contents of TNF-α and IL-6 in the spinal cord was significantly increased in groups NP,PBS and Ab (P ＜ 0.05).The PWMT was significantly higher and the contents of TNF-α and IL-6 in the spinal cord were significantly lower in group Ab than in groups NP and PBS (P ＜ 0.05).Conclusion FGF-2 is involved in the occurrence and development of NP and induction of the inflammatory response in the rat spinal cord in involved in the mechanism.