CAJ | 학술논문

目的评价不同剂量异丙酚对顺铂致大鼠肝细胞毒性的影响.方法健康雄性SD大鼠80只,体重200 ～ 250 g,3月龄,采用随机数字表法,将其随机分为8组(n=10)∶对照组(C组)、顺铂7.5 mg/kg组(Cis组)、异丙酚180 mg/kg组(P组)、脂肪乳15 ml/kg组(Ⅰ组)、顺铂7.5 mg/kg+脂肪乳15ml/kg组(CisI组)、顺铂7.5 mg/kg+异丙酚60 mg/kg组(CisP1组)、顺铂7.5 mg/kg+异丙酚120 mg/kg组(CisP2组)、顺铂7.5 mg/kg+异丙酚180 kg/mg组(CisP3组).每组均按体重予腹腔注射,C组、Cis组、P组和Ⅰ组分别单次注射生理盐水、顺铂、异丙酚和脂肪乳剂.CisI组、CisP组、CisP2组和CisP3组于注射异丙酚或脂肪乳剂后1 min注射顺铂.注射顺铂后24 h,采集下腔静脉血,全自动生化分析仪检测血浆谷丙转氨酶(ALT)及谷草转氨酶(AST)活性,取肝组织,于光镜下观察肝组织病理学结果.结果与C组比较,Cis组、CisI组、CisP1-3组血浆ALT和AST活性升高(P＜0.05),肝组织病理学损伤加重；Cis组、CisP1-3组血浆ALT和AST活性依次降低(P＜0.05),肝组织病理学损伤减轻.结论异丙酚可呈剂量依赖性地减轻顺铂致大鼠肝细胞毒性.
목적 평개불동제량이병분대순박치대서간세포독성적영향.방법 건강웅성SD대서80지,체중200 ～ 250 g,3월령,채용수궤수자표법,장기수궤분위8조(n=10)∶대조조(C조)、순박7.5 mg/kg조(Cis조)、이병분180 mg/kg조(P조)、지방유15 ml/kg조(Ⅰ조)、순박7.5 mg/kg+지방유15ml/kg조(CisI조)、순박7.5 mg/kg+이병분60 mg/kg조(CisP1조)、순박7.5 mg/kg+이병분120 mg/kg조(CisP2조)、순박7.5 mg/kg+이병분180 kg/mg조(CisP3조).매조균안체중여복강주사,C조、Cis조、P조화Ⅰ조분별단차주사생리염수、순박、이병분화지방유제.CisI조、CisP조、CisP2조화CisP3조우주사이병분혹지방유제후1 min주사순박.주사순박후24 h,채집하강정맥혈,전자동생화분석의검측혈장곡병전안매(ALT)급곡초전안매(AST)활성,취간조직,우광경하관찰간조직병이학결과.결과 여C조비교,Cis조、CisI조、CisP1-3조혈장ALT화AST활성승고(P＜0.05),간조직병이학손상가중；Cis조、CisP1-3조혈장ALT화AST활성의차강저(P＜0.05),간조직병이학손상감경.결론 이병분가정제량의뢰성지감경순박치대서간세포독성.
Objective To investigate the effect of different doses of propofol on cisplatin-induced hepatotoxicity in rats.Methods Eighty male SD rats weighing 200-250 g,aged 3 months,were randomly divided into 8 groups (n =10 each) ∶ control group (group C),cisplatin 7.5 mg/kg group (group Cis),propofol 180 mg/kg group (group P),intralipid 15 ml/kg group (group Ⅰ),cisplatin 7.5 mg/kg + intralipid 15 ml/kg group (group CisI),cisplatin 7.5 mg/kg + propofol 60 mg/kg group (group CisP1),cisplatin 7.5 mg/kg + propofol 120 mg/kg group (group CisP2),and cisplatin 7.5 mg/kg + propofol 180 mg/kg group (group CisP3).The rats in groups C,Cis,P and I received single intraperitoneal injection of normal saline,cisplatin,propofol and intralipid respectively.While in the groups CisI,CisP1,CisP2 and CisP3,rats received a single intraperitoneal injection of propofol or intralipid at 1 min before a single intraperitoneal injection of cisplatin.At 24 h after cisplatin injection,venous blood samples were taken from inferior vena cava for measurement of plasma ALT and AST activities.The liver tissues were taken for microscopic examination.Results Compared with group C,plasma ALT and AST activities were significantly increased and pathological injury was aggravated in groups Cis,CisI and CisP1-3 (P ＜ 0.05).The activities of ALT and AST were gradually decreased and the pathologic injury was attenuated in groups Cis and CisP1-3 (P ＜ 0.05).Conclusion Propofol can reduce cisplatin-induced hepatotoxicity in a dose-dependent manner in rats.