CAJ | 학술논문

目的评价脊髓背角神经元ATP敏感性钾通道(KATP通道)在大鼠开胸术后痛觉过敏中的作用.方法雄性SD大鼠,体重250 ～ 350 g,7～9周龄,开胸术后慢性痛模型制备成功后第14天行鞘内置管,置管后5d选择鞘内置管成功的大鼠28只,采用随机数字表法,将大鼠随机分为4组(n=7):空白对照组(C组)、溶媒组(DMSO组)、KATT通道开放剂吡那地尔组(P组)、KATP通道阻断剂格列本脲组(G组).DMSO组、P组和G组分别鞘内注射10％ DMSO 10μl,吡那地尔10 μg/10μl,格列本脲50 μg/10 μl.于鞘内给药前,给药后10、30、60 min时测定大鼠机械痛阈,并行冷丙酮实验,测定冷刺激痛阈.结果 4组各时点机械痛阈比较差异无统计学意义(P＞0.05).与C组和DMSO组比较,P组冷刺激痛阈升高,G组冷刺激痛阈降低(P＜0.05).C组和DMSO组冷刺激痛阈比较差异无统计学意义(P＞0.05).结论脊髓背角神经元KATP通道参与了大鼠开胸术后痛觉过敏的维持.
목적 평개척수배각신경원ATP민감성갑통도(KATP통도)재대서개흉술후통각과민중적작용.방법 웅성SD대서,체중250 ～ 350 g,7～9주령,개흉술후만성통모형제비성공후제14천행초내치관,치관후5d선택초내치관성공적대서28지,채용수궤수자표법,장대서수궤분위4조(n=7):공백대조조(C조)、용매조(DMSO조)、KATT통도개방제필나지이조(P조)、KATP통도조단제격렬본뇨조(G조).DMSO조、P조화G조분별초내주사10％ DMSO 10μl,필나지이10 μg/10μl,격렬본뇨50 μg/10 μl.우초내급약전,급약후10、30、60 min시측정대서궤계통역,병행랭병동실험,측정랭자격통역.결과 4조각시점궤계통역비교차이무통계학의의(P＞0.05).여C조화DMSO조비교,P조랭자격통역승고,G조랭자격통역강저(P＜0.05).C조화DMSO조랭자격통역비교차이무통계학의의(P＞0.05).결론 척수배각신경원KATP통도삼여료대서개흉술후통각과민적유지.
Objective To evaluate the role of ATP-sensitive potassium (KATP) channel in spinal dorsal horn neurons in hyperalgesia after thoracotomy in rats.Methods Twenty-eight Sprague-Dawley rats,aged 7-9 weeks,weighing 250-350 g,in which intrathecal catheters were successfully implanted without complications on 14th day after chronic post-thoracotomy pain was induced,were randomly divided into 4 groups (n =7 each):control group,the solvent dimethyl sulfoxide (DMSO) group,KATP channel opener pinacidil group (group P) and KATP channel blocker glibenclamide group (group G).10％ DMSO 10 μl,pinacidil 10 μg/10 μl and glibenclamide 50μg/10μ1 were injected intrathecally in groups DMSO,P and G at 5 day after the intrathecal catheter was implanted,respectively.Paw withdrawal threshold to von Frey filament stimulation was measured before intrathecal administration and at 10,30 and 60 min after intrathecal administration and the acetone test was performed.Coldinduced pain threshold was measured.Results There was no significant difference in paw withdrawal threshold to yon Frey filament stimulation at each time point among the four groups (P ＞ 0.05).Compared with C and DMSO groups,cold-induced pain threshold was significantly increased in group P and decreased in group G (P ＜ 0.05).There was no significant difference in cold-induced pain threshold between C and DMSO groups (P ＞ 0.05).Conclusion KATP channel in spinal dorsal horn neurons is involved in the maintenance of hyperalgesia after thoracotomy in rats.