中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
11期
1307-1310
,共4页
杨怀洁%柯昌斌%王雪松%钟世玉
楊懷潔%柯昌斌%王雪鬆%鐘世玉
양부길%가창빈%왕설송%종세옥
基质金属蛋白酶3%神经痛
基質金屬蛋白酶3%神經痛
기질금속단백매3%신경통
Matrix metalloproteinase 3%Neuralgia
目的 评价脊髓基质金属蛋白酶3(MMP-3)在大鼠神经病理性痛形成中的作用.方法 成年雄性Wistar大鼠180只,体重200 ～ 250 g,采用随机数字表法,将其随机分为4组(n=45):假手术组(S组)仅暴露但不结扎L5神经；神经病理性痛组(NP组)采用结扎脊髓L5神经的方法制备大鼠神经病理性痛模型；MMP-3-siRNA组(Msi组)结扎脊髓L5神经后鞘内注射MMP-3-siRNA慢病毒；MMP-3-siRNA阴性对照组(NC组)结扎脊髓L5神经后鞘内注射NC-siRNA慢病毒.于结扎后7、14、21d时测定大鼠机械痛阈,RT-PCR及Western blot法检测L4-6脊髓MMP-3和TNF-α表达.结果 与S组相比,NP组和NC组各时点机械痛阈降低,脊髓MMP-3和TNF-α蛋白及mRNA水平升高(P＜0.05)；与NP组相比,Msi组各时点机械痛阈升高,脊髓MMP-3和TNF-α蛋白和mRNA表达水平降低(P＜0.05).结论 脊髓MMP-3表达上调可激活小胶质细胞,促进其释放TNF-α,参与神经病理性痛的形成.
目的 評價脊髓基質金屬蛋白酶3(MMP-3)在大鼠神經病理性痛形成中的作用.方法 成年雄性Wistar大鼠180隻,體重200 ～ 250 g,採用隨機數字錶法,將其隨機分為4組(n=45):假手術組(S組)僅暴露但不結扎L5神經；神經病理性痛組(NP組)採用結扎脊髓L5神經的方法製備大鼠神經病理性痛模型；MMP-3-siRNA組(Msi組)結扎脊髓L5神經後鞘內註射MMP-3-siRNA慢病毒；MMP-3-siRNA陰性對照組(NC組)結扎脊髓L5神經後鞘內註射NC-siRNA慢病毒.于結扎後7、14、21d時測定大鼠機械痛閾,RT-PCR及Western blot法檢測L4-6脊髓MMP-3和TNF-α錶達.結果 與S組相比,NP組和NC組各時點機械痛閾降低,脊髓MMP-3和TNF-α蛋白及mRNA水平升高(P＜0.05)；與NP組相比,Msi組各時點機械痛閾升高,脊髓MMP-3和TNF-α蛋白和mRNA錶達水平降低(P＜0.05).結論 脊髓MMP-3錶達上調可激活小膠質細胞,促進其釋放TNF-α,參與神經病理性痛的形成.
목적 평개척수기질금속단백매3(MMP-3)재대서신경병이성통형성중적작용.방법 성년웅성Wistar대서180지,체중200 ～ 250 g,채용수궤수자표법,장기수궤분위4조(n=45):가수술조(S조)부폭로단불결찰L5신경；신경병이성통조(NP조)채용결찰척수L5신경적방법제비대서신경병이성통모형；MMP-3-siRNA조(Msi조)결찰척수L5신경후초내주사MMP-3-siRNA만병독；MMP-3-siRNA음성대조조(NC조)결찰척수L5신경후초내주사NC-siRNA만병독.우결찰후7、14、21d시측정대서궤계통역,RT-PCR급Western blot법검측L4-6척수MMP-3화TNF-α표체.결과 여S조상비,NP조화NC조각시점궤계통역강저,척수MMP-3화TNF-α단백급mRNA수평승고(P＜0.05)；여NP조상비,Msi조각시점궤계통역승고,척수MMP-3화TNF-α단백화mRNA표체수평강저(P＜0.05).결론 척수MMP-3표체상조가격활소효질세포,촉진기석방TNF-α,삼여신경병이성통적형성.
Objective To investigate the effects of matrix metalloproteinase-3 (MMP-3) in the development of neuropathic pain (NP) in rats.Methods One hundred and eighty male Wistar rats weighing 200-250 g were randomly divided into 4 groups (n =45 each):sham operation group (S group),NP group,MMP-3-siRNA group (Msi group) and MMP-3-siRNA negative control group (NC group).NP was induced by ligation of L5 spinal nerve (SNL).Lentivirus with MMP-3-siRNA was injected intrathecally after SNL in group Msi.Lentivirus with NC-siRNA was injected intrathecally after SNL in group NC.The mechanical pain threshold was measured at day 7,14 and 21 after SNL.The rats were then sacrificed after the last measurement of the mechanical pain threshold.The lumbar segment of the spinal cord (L4-6) was removed for determination of the expression of MMP-3 and TNF-a protein and mRNA by RT-PCR and Western blot.Results The mechanical pain threshold was significantly decreased and the expression of MMP-3 and TNF-α protein and mRNA was up-regulated at each time point in groups NP and NC as compared with group S (P ＜ 0.05).The mechanical pain threshold was significantly higher and the expression of MMP-3 and TNF-α protein and mRNA was lower at each time point in Msi group than in NP group (P ＜ 0.05).Conclusion Up-regulation of the expression of MMP-3 activates microglia and induces the release of TNF-α,resulting in NP in rats.
