CAJ | 학술논문

目的探讨孕早期氯胺酮麻醉对子代大鼠认知功能及海马hippyragranin (HGN) mRNA表达的影响.方法清洁级孕5～7d的SD大鼠120只,月龄7周,体重270 ～ 300 g,采用随机数表法,将其随机分为4组:氯胺酮麻醉2h组(K1)、4h组(K2)、8h组(K3)和对照组(C组),每组30只.K1组～K3组孕鼠经尾静脉注射氯胺酮20 mg/kg,翻正反射消失后,以130 mg·kg-1·h-1的速率静脉输注,维持时间分别为2、4和8h,C组给予等容量生理盐水.子鼠出生30 d时进行Morris水迷宫实验.于Morris水迷宫实验结束后第2天,处死子鼠,采用Real Time PCR法测定海马HGN mRNA的表达水平.结果与C组比较,K1组～K3组子鼠逃避潜伏期延长,穿越原平台次数和原平台所在象限停留时间缩短,海马HGN mRNA表达水平上调(P＜0.05)；K1组～K3组间子鼠逃避潜伏期、穿越原平台次数和原平台所在象限停留时间差异无统计学意义(P＞0.05).与K1组比较,K3组子鼠海马HGN mRNA表达水平上调(P＜0.05),K2组差异无统计学意义(P＞ 0.05)；K2组和K3组子鼠海马HGN mRNA表达水平差异无统计学意义(P＞0.05).结论孕早期氯胺酮麻醉对子代大鼠学习记忆功能损害的机制可能与其上调子代海马HGN mRNA的表达有关.
목적 탐토잉조기록알동마취대자대대서인지공능급해마hippyragranin (HGN) mRNA표체적영향.방법 청길급잉5～7d적SD대서120지,월령7주,체중270 ～ 300 g,채용수궤수표법,장기수궤분위4조:록알동마취2h조(K1)、4h조(K2)、8h조(K3)화대조조(C조),매조30지.K1조～K3조잉서경미정맥주사록알동20 mg/kg,번정반사소실후,이130 mg·kg-1·h-1적속솔정맥수주,유지시간분별위2、4화8h,C조급여등용량생리염수.자서출생30 d시진행Morris수미궁실험.우Morris수미궁실험결속후제2천,처사자서,채용Real Time PCR법측정해마HGN mRNA적표체수평.결과 여C조비교,K1조～K3조자서도피잠복기연장,천월원평태차수화원평태소재상한정류시간축단,해마HGN mRNA표체수평상조(P＜0.05)；K1조～K3조간자서도피잠복기、천월원평태차수화원평태소재상한정류시간차이무통계학의의(P＞0.05).여K1조비교,K3조자서해마HGN mRNA표체수평상조(P＜0.05),K2조차이무통계학의의(P＞ 0.05)；K2조화K3조자서해마HGN mRNA표체수평차이무통계학의의(P＞0.05).결론 잉조기록알동마취대자대대서학습기억공능손해적궤제가능여기상조자대해마HGN mRNA적표체유관.
Objective To investigate the effect of ketamine anesthesia in early pregnancy on expression of hippyragranin (HGN) mRNA in hippocampus in offsprings of rats.Methods One hundred and twenty pregnant Sprague-Dawley rats at 5-7 days of gestation,aged 7 weeks,weighing 270-300 g,were randomly divided into 4 groups (n =30 each):2 h anesthesia with ketamine group (group K1),4 h anesthesia with ketamine group (group K2),8 h anesthesia with ketamine group (group K3) and control group (group C).Ketamine 20 mg/kg was injected via the caudal vein,followed by continuous intravenous infusion at 130 mg· kg-1 · h-1 after loss of righting reflex,and the infusion lasted for 2,4 and 8 h in groups K1-K3,respectively.The equal volume of normal saline was given instead of ketamine in group C.The learning ability and memory of the offsprings were tested by Morris water maze test on postnatal day 30.The offsprings were sacrificed on 2nd day after the end of Morris water maze test,their brains were removed and hippocampi were isolated for determination of the expression of HGN mRNA by RT-PCR.Results Compared with group C,the escape latency was significantly prolonged,the frequency of crossing the original platform was decreased,the time of staying at the original platform quadrant was shortened,and the expression of HGN mRNA was up-regulated in groups K1-K3 (P ＜ 0.05).There was no significant difference in the escape latency,frequency of crossing the original platform and time of staying at the original platform quadrant among groups K1-K3 (P ＞ 0.05).The expression of HGN mRNA was significantly higher in group K3 that in group K1 (P ＜ 0.05).There was no significant difference in the expression of HGN mRNA between group K2 and group K3 (P ＞ 0.05).Conclusion The mechanism by which ketamine anesthesia in the early pregnancy induces impairment of learning ability and memory of the offsprings is related to up-regulation of HGN mRNA expression in hippocampus in offsprings of rats.