中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
11期
1344-1346
,共3页
氢%认知障碍%NF-κB
氫%認知障礙%NF-κB
경%인지장애%NF-κB
Hydrogen%Cognition disorders%NF-kappa B
目的 评价饱和氢盐水对老龄小鼠术后认知功能障碍(POCD)的影响.方法 C57BL/6雄性小鼠72只,18月龄,体重45 ～ 55 g,采用随机数字表法,将小鼠随机分为4组(n=18):对照组(C组)、饱和氢盐水组(H组)、POCD组和POCD+饱和氢盐水组(PH组).POCD组采用开放性胫骨骨折手术制备小鼠POCD模型,H组和PH组术前7d开始,每天早晚2次腹腔注射饱和氢盐水10ml/kg.于术后1、3和7d时各组随机取6只小鼠行水迷宫实验测定小鼠认知功能,随后处死取海马,采用ELISA法测定TNF-α和IL-1β含量,检测NF-κB活性.结果 与C组比较,POCD组术后3和7d时逃避潜伏期延长,目标象限停留时间缩短,穿越平台次数减少,术后1、3和7d时海马TNF-α、IL-1β含量及NF-κB活性升高(P＜0.05),H组各指标差异无统计学意义(P＞0.05)；与POCD组比较,PH组术后3和7d时逃避潜伏期缩短,目标象限停留时间延长,穿越平台次数增加,术后1、3和7d时海马TNF-α、IL-1β含量及NF-κB活性降低(P＜0.05).结论 饱和氢盐水可减轻小鼠术后认知功能障碍,其机制与抑制海马NK-κB活性,降低炎性反应有关.
目的 評價飽和氫鹽水對老齡小鼠術後認知功能障礙(POCD)的影響.方法 C57BL/6雄性小鼠72隻,18月齡,體重45 ～ 55 g,採用隨機數字錶法,將小鼠隨機分為4組(n=18):對照組(C組)、飽和氫鹽水組(H組)、POCD組和POCD+飽和氫鹽水組(PH組).POCD組採用開放性脛骨骨摺手術製備小鼠POCD模型,H組和PH組術前7d開始,每天早晚2次腹腔註射飽和氫鹽水10ml/kg.于術後1、3和7d時各組隨機取6隻小鼠行水迷宮實驗測定小鼠認知功能,隨後處死取海馬,採用ELISA法測定TNF-α和IL-1β含量,檢測NF-κB活性.結果 與C組比較,POCD組術後3和7d時逃避潛伏期延長,目標象限停留時間縮短,穿越平檯次數減少,術後1、3和7d時海馬TNF-α、IL-1β含量及NF-κB活性升高(P＜0.05),H組各指標差異無統計學意義(P＞0.05)；與POCD組比較,PH組術後3和7d時逃避潛伏期縮短,目標象限停留時間延長,穿越平檯次數增加,術後1、3和7d時海馬TNF-α、IL-1β含量及NF-κB活性降低(P＜0.05).結論 飽和氫鹽水可減輕小鼠術後認知功能障礙,其機製與抑製海馬NK-κB活性,降低炎性反應有關.
목적 평개포화경염수대노령소서술후인지공능장애(POCD)적영향.방법 C57BL/6웅성소서72지,18월령,체중45 ～ 55 g,채용수궤수자표법,장소서수궤분위4조(n=18):대조조(C조)、포화경염수조(H조)、POCD조화POCD+포화경염수조(PH조).POCD조채용개방성경골골절수술제비소서POCD모형,H조화PH조술전7d개시,매천조만2차복강주사포화경염수10ml/kg.우술후1、3화7d시각조수궤취6지소서행수미궁실험측정소서인지공능,수후처사취해마,채용ELISA법측정TNF-α화IL-1β함량,검측NF-κB활성.결과 여C조비교,POCD조술후3화7d시도피잠복기연장,목표상한정류시간축단,천월평태차수감소,술후1、3화7d시해마TNF-α、IL-1β함량급NF-κB활성승고(P＜0.05),H조각지표차이무통계학의의(P＞0.05)；여POCD조비교,PH조술후3화7d시도피잠복기축단,목표상한정류시간연장,천월평태차수증가,술후1、3화7d시해마TNF-α、IL-1β함량급NF-κB활성강저(P＜0.05).결론 포화경염수가감경소서술후인지공능장애,기궤제여억제해마NK-κB활성,강저염성반응유관.
Objective To investigate the effects of hydrogen-rich saline on postoperative cognitive dysfunction (POCD) in aged mice and the possible mechanism.Methods Seventy-two male C57BL/6 mice,aged 18 months,weighing 45-55 g,were randomly divided into 4 groups (n =18 each):control group (group C),hydrogen-rich saline group (group H),POCD group and POCD + hydrogen-rich saline group (group PH).POCD was induced by open tibial fracture.Hydrogen-rich saline 10 ml/kg was injected intraperitoneally twice a day for 7 days before surgery.Six mice were chosen at day 1,3 and 7 after surgery and cognitive function was assessed by Morris water maze test.The mice were then sacrificed and hippocampus was isolated for determination of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) contents (by ELISA) and nuclear factor-κB (NF-κB) activity.Results Compared with group C,the escape latency was significantly prolonged,the time of staying at the original platform quadrant was shortened,the frequency of crossing the original platform was decreased at 3 and 7 days after surgery,and the contents of NF-α and IL-1β and NF-κB activity were increased during 1,3 and 7 days after surgery in group POCD (P ＜ 0.05),and no significant change was found in the parameters mentioned above in group H (P ＞ 0.05).Compared with group POCD,the escape latency was significantly shortened,the time of staying at the original platform quadrant was prolonged,the frequency of crossing the original platform was increased at 3 and 7 days after surgery,and the contents of NF-α and IL-1β and NF-κB activity were decreased at 1,3 and 7 days after surgery.Conclusion Hydrogen-rich saline can alleviate POCD via inhibiting NK-κB activity in hippocampus and reducing inflammatory response.
