中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
11期
1393-1396
,共4页
孟叶%吕艳霞%金小雪%熊颖芬
孟葉%呂豔霞%金小雪%熊穎芬
맹협%려염하%금소설%웅영분
哌啶类%Nod1信号接头蛋白质%再灌注损伤%肾
哌啶類%Nod1信號接頭蛋白質%再灌註損傷%腎
고정류%Nod1신호접두단백질%재관주손상%신
Piperidines%Nod1 signaling adaptor protein%Reperfusion injury%Kidney
目的 探讨瑞芬太尼对肾脏缺血再灌注(I/R)损伤大鼠核苷酸结合寡聚化结构域1(NOD1)mRNA表达的影响.方法 健康成年雄性SD大鼠60只,体重220～ 250 g,采用随机数字表法,将其随机分为3组(n=20):假手术组(S组)、缺血再灌注组(1/R组)和瑞芬太尼组(R组).I/R组和R组采用夹闭双侧肾动脉45 min再灌注法制备肾脏I/R损伤模型.R组于缺血前15 min时静脉输注瑞芬太尼1.0 μg· kg-1·min-1至再灌注30 min,S组和I/R组用等容量生理盐水替代.于缺血前15 min及再灌注3、6、24 h时取肾组织,观察肾组织病理学结果,并计数中性粒细胞(PMN),采用流式细胞术双染法检测细胞凋亡率,采用RT-PCR法测定NOD1 mRNA表达水平.结果 与S组比,I/R组再灌注各时点肾组织NOD1 mRNA表达水平上调,细胞凋亡率和PMN计数升高,R组再灌注各时点肾组织细胞凋亡率和PMN计数升高,再灌注6、24 h时NOD1 mRNA表达上调(P＜0.01)；与I/R组比较,R组再灌注各时点肾组织NOD1 mRNA表达水平下调,细胞凋亡率和PMN计数降低(P＜0.05或0.01),且肾组织病理学损伤减轻.结论 瑞芬太尼通过下调NOD1 mRNA表达,抑制炎性反应和细胞凋亡,从而减轻大鼠肾脏I/R损伤.
目的 探討瑞芬太尼對腎髒缺血再灌註(I/R)損傷大鼠覈苷痠結閤寡聚化結構域1(NOD1)mRNA錶達的影響.方法 健康成年雄性SD大鼠60隻,體重220～ 250 g,採用隨機數字錶法,將其隨機分為3組(n=20):假手術組(S組)、缺血再灌註組(1/R組)和瑞芬太尼組(R組).I/R組和R組採用夾閉雙側腎動脈45 min再灌註法製備腎髒I/R損傷模型.R組于缺血前15 min時靜脈輸註瑞芬太尼1.0 μg· kg-1·min-1至再灌註30 min,S組和I/R組用等容量生理鹽水替代.于缺血前15 min及再灌註3、6、24 h時取腎組織,觀察腎組織病理學結果,併計數中性粒細胞(PMN),採用流式細胞術雙染法檢測細胞凋亡率,採用RT-PCR法測定NOD1 mRNA錶達水平.結果 與S組比,I/R組再灌註各時點腎組織NOD1 mRNA錶達水平上調,細胞凋亡率和PMN計數升高,R組再灌註各時點腎組織細胞凋亡率和PMN計數升高,再灌註6、24 h時NOD1 mRNA錶達上調(P＜0.01)；與I/R組比較,R組再灌註各時點腎組織NOD1 mRNA錶達水平下調,細胞凋亡率和PMN計數降低(P＜0.05或0.01),且腎組織病理學損傷減輕.結論 瑞芬太尼通過下調NOD1 mRNA錶達,抑製炎性反應和細胞凋亡,從而減輕大鼠腎髒I/R損傷.
목적 탐토서분태니대신장결혈재관주(I/R)손상대서핵감산결합과취화결구역1(NOD1)mRNA표체적영향.방법 건강성년웅성SD대서60지,체중220～ 250 g,채용수궤수자표법,장기수궤분위3조(n=20):가수술조(S조)、결혈재관주조(1/R조)화서분태니조(R조).I/R조화R조채용협폐쌍측신동맥45 min재관주법제비신장I/R손상모형.R조우결혈전15 min시정맥수주서분태니1.0 μg· kg-1·min-1지재관주30 min,S조화I/R조용등용량생리염수체대.우결혈전15 min급재관주3、6、24 h시취신조직,관찰신조직병이학결과,병계수중성립세포(PMN),채용류식세포술쌍염법검측세포조망솔,채용RT-PCR법측정NOD1 mRNA표체수평.결과 여S조비,I/R조재관주각시점신조직NOD1 mRNA표체수평상조,세포조망솔화PMN계수승고,R조재관주각시점신조직세포조망솔화PMN계수승고,재관주6、24 h시NOD1 mRNA표체상조(P＜0.01)；여I/R조비교,R조재관주각시점신조직NOD1 mRNA표체수평하조,세포조망솔화PMN계수강저(P＜0.05혹0.01),차신조직병이학손상감경.결론 서분태니통과하조NOD1 mRNA표체,억제염성반응화세포조망,종이감경대서신장I/R손상.
Objective To investigate the effect of remifentanil on nucleotide-binding oligomerization domain 1 (NOD1) mRNA expression in rats with renal ischemia-reperfusion (I/R) injury.Methods Sixty male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into 3 groups (n =20 each):sham operation group (S group),I/R group and remifentanil group (R group).Renal ischemia was induced by occlusion of bilateral renal arteries for 45 min followed by 24 h reperfusion in groups I/R and R.Remifentanil 1.0 μg· kg-1 · min-1 was infused until 30 min of reperfusion starting from 15 min before ischemia in group R,while the equal volume of normal saline was given instead in S and I/R groups.The animals were sacrificed at 15 min before ischemia and at 3,6,24 h of reperfusion and the kidneys were removed for microscopic examination and polymorphonuclear leukocyte (PMN) count and for measurement of NOD1 mRNA expression (by RT-PCR).The apoptotic rate was determined by flow cytometry double staining method.Results Compared with group S,NOD1 mRNA expression was up-regulated,and the apoptotic rate and PMN count were significantly increased at each time point during reperfusion in group I/R,and the apoptotic rate and PMN count were significantly increased at each time point during reperfusion,and NOD1 mRNA expression was up-regulated at 6 and 24 h of reperfusion in group R (P ＜ 0.01).Compared with I/R group,NOD1 mRNA expression was down-regulated,and the apoptotic rate and PMN count were significantly decreased at each time point during reperfusion (P ＜ 0.05 or 0.01),and the pathological changes were significantly attenuated in group R.Conclusion Remifentanil can reduce the renal I/R injury by down-regulating the expression of NOD1 mRNA and inhibiting inflammatory response and apoptosis.
