中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2013年
7期
807-809
,共3页
宿世玉%田恩琪%党秀静%孔敏%张励才
宿世玉%田恩琪%黨秀靜%孔敏%張勵纔
숙세옥%전은기%당수정%공민%장려재
神经元%瘙痒症%触液核
神經元%瘙癢癥%觸液覈
신경원%소양증%촉액핵
Neurons%Pruritus%Cerebrospinal fluid-contacting nucleus
目的 探讨触液核在大鼠慢性瘙痒维持中的作用.方法 雄性SD大鼠,3月龄,体重240～ 280 g.第一部分采用随机数字表法,将42只大鼠分为3组(n=14):生理盐水组(C组)、丙酮组(A组)和恶唑酮组(O组).O组于颈背部涂抹0.5％恶唑酮15μl,C组和A组分别涂抹等容量生理盐水和丙酮,分别于给药后7、9、13、16、17、18、21、23 d重复上述处理,记录每次给药后30 min内搔抓次数,每组取6只动物,在最后1次涂抹恶唑酮后2h取脑组织,测定触液核c-Fos表达.第二部分 采用随机数字表法,将24只大鼠分为3组(n=8):正常对照组(C1组)、慢性瘙痒组(CI组)和慢性瘙痒+触液核毁损术组(CI+ KA组),CI组和CI+ KA组采用上述方法制备慢性瘙痒模型,触液核毁损术于第8次给药后6h实施,并记录第9次给药后30 min内搔抓次数.结果 第一部分 与C组比较,A组T4-8时搔抓次数增多,O组T1-8时搔抓次数增多,A组和O组触液核c-Fos表达上调(P＜0.05)；与A组比较,O组T1-8时搔抓次数增多,触液核c-Fos表达上调(P＜0.05).第二部分 与C1组比较,CI组和CI+ KA组搔抓次数增多(P＜0.05),而CI+ KA组搔抓次数少于CI组(P＜0.05).结论 触液核参与了大鼠慢性瘙痒的维持.
目的 探討觸液覈在大鼠慢性瘙癢維持中的作用.方法 雄性SD大鼠,3月齡,體重240～ 280 g.第一部分採用隨機數字錶法,將42隻大鼠分為3組(n=14):生理鹽水組(C組)、丙酮組(A組)和噁唑酮組(O組).O組于頸揹部塗抹0.5％噁唑酮15μl,C組和A組分彆塗抹等容量生理鹽水和丙酮,分彆于給藥後7、9、13、16、17、18、21、23 d重複上述處理,記錄每次給藥後30 min內搔抓次數,每組取6隻動物,在最後1次塗抹噁唑酮後2h取腦組織,測定觸液覈c-Fos錶達.第二部分 採用隨機數字錶法,將24隻大鼠分為3組(n=8):正常對照組(C1組)、慢性瘙癢組(CI組)和慢性瘙癢+觸液覈燬損術組(CI+ KA組),CI組和CI+ KA組採用上述方法製備慢性瘙癢模型,觸液覈燬損術于第8次給藥後6h實施,併記錄第9次給藥後30 min內搔抓次數.結果 第一部分 與C組比較,A組T4-8時搔抓次數增多,O組T1-8時搔抓次數增多,A組和O組觸液覈c-Fos錶達上調(P＜0.05)；與A組比較,O組T1-8時搔抓次數增多,觸液覈c-Fos錶達上調(P＜0.05).第二部分 與C1組比較,CI組和CI+ KA組搔抓次數增多(P＜0.05),而CI+ KA組搔抓次數少于CI組(P＜0.05).結論 觸液覈參與瞭大鼠慢性瘙癢的維持.
목적 탐토촉액핵재대서만성소양유지중적작용.방법 웅성SD대서,3월령,체중240～ 280 g.제일부분채용수궤수자표법,장42지대서분위3조(n=14):생리염수조(C조)、병동조(A조)화악서동조(O조).O조우경배부도말0.5％악서동15μl,C조화A조분별도말등용량생리염수화병동,분별우급약후7、9、13、16、17、18、21、23 d중복상술처리,기록매차급약후30 min내소조차수,매조취6지동물,재최후1차도말악서동후2h취뇌조직,측정촉액핵c-Fos표체.제이부분 채용수궤수자표법,장24지대서분위3조(n=8):정상대조조(C1조)、만성소양조(CI조)화만성소양+촉액핵훼손술조(CI+ KA조),CI조화CI+ KA조채용상술방법제비만성소양모형,촉액핵훼손술우제8차급약후6h실시,병기록제9차급약후30 min내소조차수.결과 제일부분 여C조비교,A조T4-8시소조차수증다,O조T1-8시소조차수증다,A조화O조촉액핵c-Fos표체상조(P＜0.05)；여A조비교,O조T1-8시소조차수증다,촉액핵c-Fos표체상조(P＜0.05).제이부분 여C1조비교,CI조화CI+ KA조소조차수증다(P＜0.05),이CI+ KA조소조차수소우CI조(P＜0.05).결론 촉액핵삼여료대서만성소양적유지.
Objective To evaluate the role of cerebrospinal fluid-contacting nuclei (CSF-CN) in maintenance of chronic itch in rats.Methods Forty-two adult male Sprague-Dawley rats,aged 3 months,weighing 240-280 g,were used in the study.The experiment was performed in two parts.Part Ⅰ Forty-two Sprague-Dawley rats were randomly divided into 3 groups (n =14 each):control group (C group),acetone group (A group) and oxazolone group (O group).0.5％ oxazolone 15 μl was applied to the neck and back of rats in group O,while the equal volume of normal saline and acetone was applied in groups C and A,respectively.Application of the drug mentioned above was repeated on day 7,9,13,16,17,18,21 and 23 after the first stimulation in each rat in each group.Scratching behaviors were oberserved within 30 min after each stimulaiton.Six rats in each group were chosen and sacrificed after the last application of oxazolone,and the brains were obtained for determination of c-Fos expression in CSF-CN.Part Ⅱ Twenty-four rats were randomly divided into 3 groups (n =8 each):control group (C1 group),chronic itch group (group CI),and chronic itch + lesion group (CI + KA group).Chronic itch was induced by repeated application of oxazolone as previously described in CI and CI + KA groups.The chemical lesion of CSF-CN was performed at 6 h after 8th application of the drug.Then the scratching behaviors were observed within 30 min after 9th application of the drug.Results Part Ⅰ Compared with C group,the scratching behaviors were increased significantly at T4-8 in A group,and at T1-8 in O group (P ＜ 0.05),and the expression of c-Fos was up-regulated in O and A groups (P ＜ 0.05).Compared with A group,the scratching behaviors were increased significantly at T1-8 and the expression of c-Fos was up-regulated in O group (P ＜ 0.05).Part Ⅱ Compared with C1 group,the scratching behaviors were significantly increased in CI and CI + KA groups (P ＜ 0.05).The scratching behaviors were significantly reduced in CI + KA group compared with CI group (P ＜ 0.05).Conclusion CSF-CN is involved in the maintenance of chronic itch in rats.
