中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2013年
8期
986-988
,共3页
自由基清除剂%脓毒症%血脑屏障
自由基清除劑%膿毒癥%血腦屏障
자유기청제제%농독증%혈뇌병장
Free radical scavengers%Sepsis%Blood-brain barrier
目的 探讨依达拉奉对脓毒症大鼠血脑屏障通透性的影响.方法 雄性SD大鼠90只,体重200～250g,采用随机数字表法,将其分为3组(n=30):对照组(C组)、脓毒症组(LPS组)和依达拉奉组(E组).LPS组和E组经股静脉注射LPS 10 mg/kg制备大鼠脓毒症模型.E组于造模后静脉注射依达拉奉3 mg/kg,每隔2h注射1次,共注射7次；C组和LPS组每隔2h注射等容量生理盐水,共注射7次.分别于造模后2、6和12h时,取5只大鼠,股静脉注射2％伊文思兰(EB)3ml/kg,然后处死取脑组织,测定EB含量和脑含水量.分别于造模后2、6和12h时,取5只大鼠,采集股动脉血样,测定血清MDA浓度,然后处死取脑组织,光镜下观察病理学结果.结果 与C组比较,LPS组和E组造模后6和12h时脑含水量和EB含量升高,造模后2、6和12h时血清MDA浓度升高(P＜0.05)；与LPS组比较,E组造模后6和12h时脑含水量和EB含量降低,造模后2、6和12h时血清MDA浓度降低(P＜0.05),病理学损伤减轻.结论 依达拉奉可降低血脑屏障通透性,减轻脑水肿,从而减轻脓毒症大鼠脑损伤,机制可能与减少氧自由基的生成有关.
目的 探討依達拉奉對膿毒癥大鼠血腦屏障通透性的影響.方法 雄性SD大鼠90隻,體重200～250g,採用隨機數字錶法,將其分為3組(n=30):對照組(C組)、膿毒癥組(LPS組)和依達拉奉組(E組).LPS組和E組經股靜脈註射LPS 10 mg/kg製備大鼠膿毒癥模型.E組于造模後靜脈註射依達拉奉3 mg/kg,每隔2h註射1次,共註射7次；C組和LPS組每隔2h註射等容量生理鹽水,共註射7次.分彆于造模後2、6和12h時,取5隻大鼠,股靜脈註射2％伊文思蘭(EB)3ml/kg,然後處死取腦組織,測定EB含量和腦含水量.分彆于造模後2、6和12h時,取5隻大鼠,採集股動脈血樣,測定血清MDA濃度,然後處死取腦組織,光鏡下觀察病理學結果.結果 與C組比較,LPS組和E組造模後6和12h時腦含水量和EB含量升高,造模後2、6和12h時血清MDA濃度升高(P＜0.05)；與LPS組比較,E組造模後6和12h時腦含水量和EB含量降低,造模後2、6和12h時血清MDA濃度降低(P＜0.05),病理學損傷減輕.結論 依達拉奉可降低血腦屏障通透性,減輕腦水腫,從而減輕膿毒癥大鼠腦損傷,機製可能與減少氧自由基的生成有關.
목적 탐토의체랍봉대농독증대서혈뇌병장통투성적영향.방법 웅성SD대서90지,체중200～250g,채용수궤수자표법,장기분위3조(n=30):대조조(C조)、농독증조(LPS조)화의체랍봉조(E조).LPS조화E조경고정맥주사LPS 10 mg/kg제비대서농독증모형.E조우조모후정맥주사의체랍봉3 mg/kg,매격2h주사1차,공주사7차；C조화LPS조매격2h주사등용량생리염수,공주사7차.분별우조모후2、6화12h시,취5지대서,고정맥주사2％이문사란(EB)3ml/kg,연후처사취뇌조직,측정EB함량화뇌함수량.분별우조모후2、6화12h시,취5지대서,채집고동맥혈양,측정혈청MDA농도,연후처사취뇌조직,광경하관찰병이학결과.결과 여C조비교,LPS조화E조조모후6화12h시뇌함수량화EB함량승고,조모후2、6화12h시혈청MDA농도승고(P＜0.05)；여LPS조비교,E조조모후6화12h시뇌함수량화EB함량강저,조모후2、6화12h시혈청MDA농도강저(P＜0.05),병이학손상감경.결론 의체랍봉가강저혈뇌병장통투성,감경뇌수종,종이감경농독증대서뇌손상,궤제가능여감소양자유기적생성유관.
Objective To evaluate the effects of edaravone on the permeability of blood-brain barrier in septic rats.Methods Ninety male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 3 groups (n=30 each):control group (group C),sepsis group (group lipopolysaccharide (LPS)) and edaravone group (group E).Sepsis was induced by injection of LPS 10 mg/kg via the femoral vein in LPS and E groups.After LPS injection,edaravone 3.0 mg/kg was injected intravenously every 2h for 7 times in group E.The equal volume of normal saline was administered instead of edaravone in C and LPS groups.At 2,6 and 12h after LPS injection,5 rats were chosen and Evan's blue (EB) was injected via the femoral vein,and then the rats were sacrificed and brain tissues were removed for determination of EB and water contents.Another 5 rats were chosen and blood samples were taken from the femoral artery for measurement of serum MDA concentration,and then the rats were sacrificed and the brain tissue was harvested for microscopic examination.Results Compared with group C,brain water and EB contents were significantly increased at 6 and 12h after LPS injection,and the serum MDA concentration was increased at 2,6 and 12h after LPS injection in LPS and E groups (P ＜ 0.05).Compared with group LPS,brain water and EB contents were significantly decreased at 6 and 12h after LPS injection,and serum MDA concentrations were decreased at 2,6 and 12h after LPS injection in group E (P ＜ 0.05).Sepsis-induced pathological changes were significantly attenuated in group E.Conclusion Edaravone can decrease the permeability of blood-brain barrier,attenuate brain edema and brain injury in septic rats,and reduction of oxygen free radical production may be involved in the mechanism.
