CAJ | 학술논문

目的评价阿片受体在芬太尼抑制人胃癌MGC-803细胞增殖和迁移中的作用.方法人胃癌MGC-803细胞接种于培养板中,采用随机数字表法,将其分为4组(n=54):对照组(C组)、芬太尼组(F组)、纳洛酮组(N组)和纳洛酮+芬太尼组(NF组).F组和N组分别在培养液中加入芬太尼或纳洛酮,终浓度分别为0.1 μmol/L或10μmol/L; NF组培养液中加入纳洛酮,终浓度10 μmol/L,孵育30 min时,将芬太尼加入培养液,终浓度0.1μmol/L,继续孵育.于孵育12、24、36、48、60和72 h时分别测定细胞活力,孵育24h时测定细胞凋亡情况,孵育48 h时测定细胞划痕愈合情况,孵育7d时测定克隆形成情况.结果与C组比较,N组细胞活力、克隆形成率、细胞凋亡率和细胞划痕愈合率差异无统计学意义(P＞0.05),F组和NF组细胞活力、克隆形成率和细胞划痕愈合率降低,细胞凋亡率升高(P＜0.05)；与F组比较,NF组细胞活力、克隆形成率、细胞划痕愈合率及细胞凋亡率差异无统计学意义(P＞0.05).结论在体外,芬太尼抑制人胃癌MGC-803细胞增殖和迁移的作用与阿片受体途径无关.
목적 평개아편수체재분태니억제인위암MGC-803세포증식화천이중적작용.방법 인위암MGC-803세포접충우배양판중,채용수궤수자표법,장기분위4조(n=54):대조조(C조)、분태니조(F조)、납락동조(N조)화납락동+분태니조(NF조).F조화N조분별재배양액중가입분태니혹납락동,종농도분별위0.1 μmol/L혹10μmol/L; NF조배양액중가입납락동,종농도10 μmol/L,부육30 min시,장분태니가입배양액,종농도0.1μmol/L,계속부육.우부육12、24、36、48、60화72 h시분별측정세포활력,부육24h시측정세포조망정황,부육48 h시측정세포화흔유합정황,부육7d시측정극륭형성정황.결과 여C조비교,N조세포활력、극륭형성솔、세포조망솔화세포화흔유합솔차이무통계학의의(P＞0.05),F조화NF조세포활력、극륭형성솔화세포화흔유합솔강저,세포조망솔승고(P＜0.05)；여F조비교,NF조세포활력、극륭형성솔、세포화흔유합솔급세포조망솔차이무통계학의의(P＞0.05).결론 재체외,분태니억제인위암MGC-803세포증식화천이적작용여아편수체도경무관.
Objective To evaluate the role of opioid receptors in fentanyl-induced inhibition of proliferation and migration of human gastric cancer cell line MGC-803.Methods The human gastric cancer cell line MGC-803 was cultured in DMEM liquid culture medium.The cells were seeded in 6-well or 96-well plates and then randomly divided into 4 groups (n =54 each):control group (group C),fentanyl group (group F),naloxon group (group N) and naloxon + fentanyl group (group NF).The cells were exposed to 0.1 μmol/L fentanyl and 10 μmol/L naloxon in F and N groups,respectively.The cells were incubated with 10 μmnol/L naloxon for 30 min and then O.1 μmol/L fentanyl was added to the culture medium in group NF.The viability of the cells was detected by MTT assay after being incubated with fentanyl for 12,24,36,48,60 and 72 h.The cell apoptosis was assessed by flow cytometry after being incubated with fentanyl for 24 h.The migration of the cells was detected by wound healing assay after being incubated with fentanyl for 48 h.The proliferation of the cells was determined by colony formation assay at 7 day of incubation with fentanyl.Results Compared with group C,no significant changes in the viability of the cells,rate of colony formation,apoptotic rate and rate of cell wound healing were found in group N (P ＞ 0.05),and the viability of the cells,rate of colony formation and rate of cell wound healing were significantly decreased,and the apoptotic rate was increased in F and NF groups (P ＜ 0.05).There was no significant difference in the viability of the cells,rate of colony formation,rate of cell wound healing and apoptotic rate between group NF and group F (P ＞ 0.05).Conclusion Opioid receptors are not involved in fentanyl-induced inhibition of proliferation and migration of human gastric cancer cell line MGC-803 in vitro.