中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2013年
10期
1202-1205
,共4页
王惠枢%徐世元%张良成%郑艇%钟毅%蒋焕伟
王惠樞%徐世元%張良成%鄭艇%鐘毅%蔣煥偉
왕혜추%서세원%장량성%정정%종의%장환위
右美托咪啶%烧伤%心肌%细胞凋亡
右美託咪啶%燒傷%心肌%細胞凋亡
우미탁미정%소상%심기%세포조망
Dexmedetomidine%Burns%Myocardium%Apoptosis
目的 评价右美托咪定对严重烫伤大鼠心肌细胞凋亡的影响.方法 健康成年雄性SD大鼠18只,体重220 ~ 280 g,采用随机数字表法,将其分为3组(n=6):对照组(C组)、烫伤组(B组)、烫伤+右美托咪定30 μg/kg组(D组).建立大鼠30%体表面积烫伤模型,致伤后按Parkland公式补液抗休克治疗.D组伤后即刻腹腔注射右美托咪定30 μg/kg,B组腹腔注射等容量生理盐水.于烫伤后12h取左心室心肌组织,光学显微镜观察心肌组织病理学结果,应用TUNEL法确定心肌细胞凋亡指数,应用Western blot法检测心肌葡萄糖调节蛋白78 (GRP78)、C/EBP同源蛋白(CHOP)表达.结果 与C组比较,B组和D组心肌细胞凋亡指数升高、心肌GRP78及CHOP表达上调(P<0.05);与B组比较,D组心肌细胞凋亡指数降低、心肌GRP78与CHOP表达下调(P<0.05).B组心肌病理学损伤明显,D组心肌病理学损伤明显减轻.结论 右美托咪定可通过抑制严重烫伤大鼠内质网应激介导的心肌细胞凋亡,发挥心肌保护作用.
目的 評價右美託咪定對嚴重燙傷大鼠心肌細胞凋亡的影響.方法 健康成年雄性SD大鼠18隻,體重220 ~ 280 g,採用隨機數字錶法,將其分為3組(n=6):對照組(C組)、燙傷組(B組)、燙傷+右美託咪定30 μg/kg組(D組).建立大鼠30%體錶麵積燙傷模型,緻傷後按Parkland公式補液抗休剋治療.D組傷後即刻腹腔註射右美託咪定30 μg/kg,B組腹腔註射等容量生理鹽水.于燙傷後12h取左心室心肌組織,光學顯微鏡觀察心肌組織病理學結果,應用TUNEL法確定心肌細胞凋亡指數,應用Western blot法檢測心肌葡萄糖調節蛋白78 (GRP78)、C/EBP同源蛋白(CHOP)錶達.結果 與C組比較,B組和D組心肌細胞凋亡指數升高、心肌GRP78及CHOP錶達上調(P<0.05);與B組比較,D組心肌細胞凋亡指數降低、心肌GRP78與CHOP錶達下調(P<0.05).B組心肌病理學損傷明顯,D組心肌病理學損傷明顯減輕.結論 右美託咪定可通過抑製嚴重燙傷大鼠內質網應激介導的心肌細胞凋亡,髮揮心肌保護作用.
목적 평개우미탁미정대엄중탕상대서심기세포조망적영향.방법 건강성년웅성SD대서18지,체중220 ~ 280 g,채용수궤수자표법,장기분위3조(n=6):대조조(C조)、탕상조(B조)、탕상+우미탁미정30 μg/kg조(D조).건립대서30%체표면적탕상모형,치상후안Parkland공식보액항휴극치료.D조상후즉각복강주사우미탁미정30 μg/kg,B조복강주사등용량생리염수.우탕상후12h취좌심실심기조직,광학현미경관찰심기조직병이학결과,응용TUNEL법학정심기세포조망지수,응용Western blot법검측심기포도당조절단백78 (GRP78)、C/EBP동원단백(CHOP)표체.결과 여C조비교,B조화D조심기세포조망지수승고、심기GRP78급CHOP표체상조(P<0.05);여B조비교,D조심기세포조망지수강저、심기GRP78여CHOP표체하조(P<0.05).B조심기병이학손상명현,D조심기병이학손상명현감경.결론 우미탁미정가통과억제엄중탕상대서내질망응격개도적심기세포조망,발휘심기보호작용.
Objective To evaluate the effect of dexmedetomidine on apoptosis in myocardial cells in rats with severe scald.Methods Eighteen healthy male Sprague-Dawley rats,weighing 220-280 g,were randomly divided into 3 groups (n =6 each) using a random number table:control group (group C),scald group (group B)and scald + dexmedetomidine 30 μg/kg group (group D).Thirty percent of the total body surface was shaved and then exposed to 94 ℃ water for 12 s.Rats were resuscitated with isotonic saline according to Parkland formula immediately after burn.Sham burn was produced in C group.In group D,the rats received inraperitoneal injection of dexmedetomidine 30 μg/kg immediately after burn,and the equal volume of normal saline was injected in group B.The left ventricle was removed at 12 h after burn to observe the pathological changes of myocardial tissues with light microscope and to detect the apoptosis in myocardial cells (TUNEL assay) and expression of glucose-regulated protein 78 (GRP78) and C/EBP-homologous protein (CHOP) (using Western blot).The apoptosis index was calculated.Results Compared with group C,the apoptosis index was significantly increased and the expression of GRP78 and CHOP was up-regulated in B and D groups (P < 0.05).Compared with group B,the apoptosis index was significantly decreased and the expression of GRP78 and CHOP was down-regulated in group D (P < 0.05).The pathological changes were obvious in group B and were significantly attenuated in group D.Conclusion Dexmedetomidine can protect myocardium through inhibiting endoplasmic reticulum stress-mediated apoptosis in myocardial cells in rats with severe scald.