CAJ | 학술논문

目的评价脊髓哺乳动物雷帕霉素靶蛋白(mTOR)信号通路在大鼠神经病理性痛形成中的作用.方法清洁级健康成年雄性SD大鼠20只,体重180 ～ 220 g,采用随机数字表法分为4组(n=5):对照组(C组)、假手术组(S组)、神经病理性痛组(NP组)和雷帕霉素组(R组).除C组外,各组大鼠均行鞘内置管术成功后观察3d.S组仅分离坐骨神经不结扎;NP组采用结扎坐骨神经的方法制备大鼠神经病理性痛模型;R组结扎坐骨神经后4h鞘内注射4％雷帕霉素10μl,连续3d.在结扎坐骨神经前1d和结扎后3、5和7d时测定机械痛阈,定量分析脊髓背角Ⅱ层神经元的细胞器和突触结构变化.结果与C组和S组比较,NP组和R组结扎后5和7d时机械痛阈降低,总突触、棘突触、凹型突触、穿孔性突触密度及突触后致密区厚度、突触界面曲率增加(P＜ 0.05或0.01);与NP组比较,R组结扎后5和7d时机械痛阈升高,总突触、棘突触、凹型突触、穿孔性突触密度及突触后致密区厚度、突触界面曲率降低(P＜0.05).结论脊髓mTOR信号通路可能通过调节脊髓背角突触结构可塑性参与大鼠神经病理性痛的形成.
목적 평개척수포유동물뢰파매소파단백(mTOR)신호통로재대서신경병이성통형성중적작용.방법 청길급건강성년웅성SD대서20지,체중180 ～ 220 g,채용수궤수자표법분위4조(n=5):대조조(C조)、가수술조(S조)、신경병이성통조(NP조)화뢰파매소조(R조).제C조외,각조대서균행초내치관술성공후관찰3d.S조부분리좌골신경불결찰;NP조채용결찰좌골신경적방법제비대서신경병이성통모형;R조결찰좌골신경후4h초내주사4％뢰파매소10μl,련속3d.재결찰좌골신경전1d화결찰후3、5화7d시측정궤계통역,정량분석척수배각Ⅱ층신경원적세포기화돌촉결구변화.결과 여C조화S조비교,NP조화R조결찰후5화7d시궤계통역강저,총돌촉、극돌촉、요형돌촉、천공성돌촉밀도급돌촉후치밀구후도、돌촉계면곡솔증가(P＜ 0.05혹0.01);여NP조비교,R조결찰후5화7d시궤계통역승고,총돌촉、극돌촉、요형돌촉、천공성돌촉밀도급돌촉후치밀구후도、돌촉계면곡솔강저(P＜0.05).결론 척수mTOR신호통로가능통과조절척수배각돌촉결구가소성삼여대서신경병이성통적형성.
Objective To investigate the role of mammlian target of rapamycin (mTOR) signaling pathway in the spinal cord in the development of neuropathic pain (NP) in rats.Methods Twenty adult male SpragueDawley rats,weighing 180-220 g,were randomly divided into d groups (n =5 each) using a random number table:control group (group C),sham operation group (group S),NP group,and rapamycin group (group R).Intrathecal catheters were successfully implanted in the rats except those in group C and observed for 3 days.In group S,the left sciatic nerve was only exposed but not ligated.NP was produced by clamping the left sciatic nerve.In group R,rapamycin 10 μl was injected intrathecally at 4 h after NP for 3 consecutive days.At 1 day before operation and 3,5 and 7 days after NP,the mechanical pain threshold (MPT) was measured and the changes in synaptic structure and organelles in the 1amine Ⅱ of the spinal dorsal horn were studied with transmission electron microscope and stereological analysis.Results Compared with C and S groups,MPT was significantly decreased,the numerical density of all synapses,spine synapses,negative synapses and perforated synapses,thickness of the postsynaptic density and curvature of the synaptic interface were increased at 5 and 7 days after ligation in NP and R groups (P ＜ 0.05 or 0.01).Compared with NP group,MPT was significantly increased,the numerical density of all synapses,spine synapses,negative synapses and perforated synapses,thickness of the postsynaptic density and curvature of the synaptic interface were decreased at 5 and 7 days after ligation in R group (P ＜ 0.05).Conclusion mTOR signaling pathway in the spinal cord is involved in the development of NP in rats possibility through regulating synaptic plasticity in the spinal dorsal horn.