中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2013年
12期
1481-1484
,共4页
麻醉药,吸入%缺血预处理%呼吸窘迫综合征,成人%肾%再灌注损伤
痳醉藥,吸入%缺血預處理%呼吸窘迫綜閤徵,成人%腎%再灌註損傷
마취약,흡입%결혈예처리%호흡군박종합정,성인%신%재관주손상
Anesthetics,inhalation%Ischemic preconditioning%Respiratory distress syndrome,adult%Kidney%Reperfusion injury
目的 评价七氟醚预处理对大鼠肾脏缺血再灌注诱发肺损伤的影响.方法 雄性Wistar大鼠40只,3月龄,体重200 ～ 250 g,采用随机数字表法,将其分为5组(n=8):假手术组(S组)、肾脏缺血再灌注组(I/R组)、1.1％七氟醚预处理组(SP1组)、2.2％七氟醚预处理组(SP2组)和3.3％七氟醚预处理组(SP3组).采用阻断双侧肾动脉45 min再灌注6h的方法制备肾脏缺血再灌注损伤模型.SP1组-SP3组在肾缺血前分别吸入相应浓度七氟醚1h,洗脱10 min.再灌注6h时处死大鼠,取肺组织,HE染色,光学显微镜下观察肺组织病理学结果,进行肺损伤评分,测定肺湿/干重(W/D)比、髓过氧化物酶(MPO)活性和血管紧张素转化酶(ACE)mRNA表达.结果 与S组比较,I/R组、SP1组、SP2组和SP3组肺损伤评分、W/D比、MPO和ACE mRNA表达升高(P＜0.05);与I/R组比较,SP1组、SP2组和SP3组肺损伤评分、W/D比、MPO和ACE mRNA表达降低(P＜0.05);与SP1组及SP3组比较,SP组肺损伤评分、W/D比、MPO和ACE mRNA表达降低(P＜0.05);与SP1组比较,SP3组肺组织W/D比降低(P＜0.05),其余指标差异无统计学意义(P＞0.05).结论 七氟醚预处理可减轻大鼠肾脏缺血再灌注诱发肺损伤,其机制与抑制ACE合成有关.
目的 評價七氟醚預處理對大鼠腎髒缺血再灌註誘髮肺損傷的影響.方法 雄性Wistar大鼠40隻,3月齡,體重200 ～ 250 g,採用隨機數字錶法,將其分為5組(n=8):假手術組(S組)、腎髒缺血再灌註組(I/R組)、1.1％七氟醚預處理組(SP1組)、2.2％七氟醚預處理組(SP2組)和3.3％七氟醚預處理組(SP3組).採用阻斷雙側腎動脈45 min再灌註6h的方法製備腎髒缺血再灌註損傷模型.SP1組-SP3組在腎缺血前分彆吸入相應濃度七氟醚1h,洗脫10 min.再灌註6h時處死大鼠,取肺組織,HE染色,光學顯微鏡下觀察肺組織病理學結果,進行肺損傷評分,測定肺濕/榦重(W/D)比、髓過氧化物酶(MPO)活性和血管緊張素轉化酶(ACE)mRNA錶達.結果 與S組比較,I/R組、SP1組、SP2組和SP3組肺損傷評分、W/D比、MPO和ACE mRNA錶達升高(P＜0.05);與I/R組比較,SP1組、SP2組和SP3組肺損傷評分、W/D比、MPO和ACE mRNA錶達降低(P＜0.05);與SP1組及SP3組比較,SP組肺損傷評分、W/D比、MPO和ACE mRNA錶達降低(P＜0.05);與SP1組比較,SP3組肺組織W/D比降低(P＜0.05),其餘指標差異無統計學意義(P＞0.05).結論 七氟醚預處理可減輕大鼠腎髒缺血再灌註誘髮肺損傷,其機製與抑製ACE閤成有關.
목적 평개칠불미예처리대대서신장결혈재관주유발폐손상적영향.방법 웅성Wistar대서40지,3월령,체중200 ～ 250 g,채용수궤수자표법,장기분위5조(n=8):가수술조(S조)、신장결혈재관주조(I/R조)、1.1％칠불미예처리조(SP1조)、2.2％칠불미예처리조(SP2조)화3.3％칠불미예처리조(SP3조).채용조단쌍측신동맥45 min재관주6h적방법제비신장결혈재관주손상모형.SP1조-SP3조재신결혈전분별흡입상응농도칠불미1h,세탈10 min.재관주6h시처사대서,취폐조직,HE염색,광학현미경하관찰폐조직병이학결과,진행폐손상평분,측정폐습/간중(W/D)비、수과양화물매(MPO)활성화혈관긴장소전화매(ACE)mRNA표체.결과 여S조비교,I/R조、SP1조、SP2조화SP3조폐손상평분、W/D비、MPO화ACE mRNA표체승고(P＜0.05);여I/R조비교,SP1조、SP2조화SP3조폐손상평분、W/D비、MPO화ACE mRNA표체강저(P＜0.05);여SP1조급SP3조비교,SP조폐손상평분、W/D비、MPO화ACE mRNA표체강저(P＜0.05);여SP1조비교,SP3조폐조직W/D비강저(P＜0.05),기여지표차이무통계학의의(P＞0.05).결론 칠불미예처리가감경대서신장결혈재관주유발폐손상,기궤제여억제ACE합성유관.
Objective To evaluate the effects of sevoflurane preconditioning on lung injury induced by renal ischemia-reperfusion (I/R) in rats.Methods Forty adult male Wistar rats,aged 3 months,weighing 200-250 g,were randomly divided into 5 groups (n =8 each):sham operation group (group S); renal I/R group (group I/R); 1.1％ sevoflurane preconditioning group (group SP1); 2.2％ sevoflurane preconditioning group (group SP2); and 3.3％ sevoflurane preconditioning group (group SP3).Renal I/R was induced by clamping bilateral renal pedicles for 45 min followed by 6 h reperfusion.In SP1-SP3 groups,1.1％,2.2％ and 3.3％ sevoflurane were inhaled for 1 h,respectively,before ischemia,followed by 10 min washout.The rats were sacrificed by exsanguination at 6 h of reperfusion,and lungs were removed for determination of wet/dry lung weight (W/D) ratio,myeloperoxidase (MPO) activity and expression of angiotensin converting enzyme (ACE) mRNA,and for microscope examination of pathologic changes which were scored.Results Compared with group S,the pathological score,W/D ratio,MPO activity and expression of ACE mRNA were significantly increased in I/R and SP1-SP3 groups (P ＜ 0.05).Compared with group I/R,the pathological score,W/D ratio,MPO activity and expression of ACE mRNA were significantly decreased in SP1-SP3 groups (P ＜ 0.05).Compared with SP1 and SP3 groups,the pathological score,W/D ratio,MPO activity and expression of ACE mRNA were significantly decreased in group SP2 (P ＜ 0.05).Compared with group SP1,the W/D ratio was significantly decreased (P ＜0.05),and no significant changes were found in the other parameters in group SP3 (P ＞ 0.05).Conclusion Sevoflurane preconditioning can attenuate lung injury induced by renal I/R and inhibition of ACE synthesis is involved in the mechanism.
