中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
8期
1017-1019
,共3页
张莉%张勇%唐晓%高天勤%王耀岐
張莉%張勇%唐曉%高天勤%王耀岐
장리%장용%당효%고천근%왕요기
右美托咪啶%心肌再灌注损伤%肾
右美託咪啶%心肌再灌註損傷%腎
우미탁미정%심기재관주손상%신
Dexmedetomidine%Myocardial reperfusion injury%Renal
目的 评价右美托咪定对肾缺血再灌注诱发大鼠心肌损伤的影响.方法 成年雄性Wistar大鼠24只,体重280 ~ 300 g,采用随机数字表法,将其分为3组(n=8):假手术组(S组)、肾缺血再灌注组(I/R组)、右美托咪定组(Dex组).S组仅游离双侧肾动脉,不结扎.I/R组用无创动脉夹结扎大鼠双侧肾动脉,缺血45 min后恢复灌注.Dex组于缺血前20 min腹腔注射右美托咪定50μg/kg,其余处理同I/R组.于再灌注24 h时取心肌组织,采用硫代巴比妥法测定心肌MDA含量,黄嘌呤氧化酶法测定SOD活性,采用流式细胞术检测心肌细胞凋亡情况,计算凋亡指数(AI),采用免疫组化法测定心肌Bcl-2及Bax的表达,计算Bcl-2/Bax比值.结果 与S组相比,I/R组和Dex组AI和心肌MDA含量升高,SOD活性降低,I/R组Bcl-2/Bax比值降低,Dex组Bcl-2/Bax比值升高(P<0.05);与 I/R组相比,Dex组AI和心肌MDA含量降低,SOD活性和Bcl-2/Bax比值升高(P<0.05).结论 右美托咪定可减轻肾缺血再灌注诱发大鼠心肌损伤,其机制可能与抑制心肌细胞凋亡和减轻脂质过氧化反应有关.
目的 評價右美託咪定對腎缺血再灌註誘髮大鼠心肌損傷的影響.方法 成年雄性Wistar大鼠24隻,體重280 ~ 300 g,採用隨機數字錶法,將其分為3組(n=8):假手術組(S組)、腎缺血再灌註組(I/R組)、右美託咪定組(Dex組).S組僅遊離雙側腎動脈,不結扎.I/R組用無創動脈夾結扎大鼠雙側腎動脈,缺血45 min後恢複灌註.Dex組于缺血前20 min腹腔註射右美託咪定50μg/kg,其餘處理同I/R組.于再灌註24 h時取心肌組織,採用硫代巴比妥法測定心肌MDA含量,黃嘌呤氧化酶法測定SOD活性,採用流式細胞術檢測心肌細胞凋亡情況,計算凋亡指數(AI),採用免疫組化法測定心肌Bcl-2及Bax的錶達,計算Bcl-2/Bax比值.結果 與S組相比,I/R組和Dex組AI和心肌MDA含量升高,SOD活性降低,I/R組Bcl-2/Bax比值降低,Dex組Bcl-2/Bax比值升高(P<0.05);與 I/R組相比,Dex組AI和心肌MDA含量降低,SOD活性和Bcl-2/Bax比值升高(P<0.05).結論 右美託咪定可減輕腎缺血再灌註誘髮大鼠心肌損傷,其機製可能與抑製心肌細胞凋亡和減輕脂質過氧化反應有關.
목적 평개우미탁미정대신결혈재관주유발대서심기손상적영향.방법 성년웅성Wistar대서24지,체중280 ~ 300 g,채용수궤수자표법,장기분위3조(n=8):가수술조(S조)、신결혈재관주조(I/R조)、우미탁미정조(Dex조).S조부유리쌍측신동맥,불결찰.I/R조용무창동맥협결찰대서쌍측신동맥,결혈45 min후회복관주.Dex조우결혈전20 min복강주사우미탁미정50μg/kg,기여처리동I/R조.우재관주24 h시취심기조직,채용류대파비타법측정심기MDA함량,황표령양화매법측정SOD활성,채용류식세포술검측심기세포조망정황,계산조망지수(AI),채용면역조화법측정심기Bcl-2급Bax적표체,계산Bcl-2/Bax비치.결과 여S조상비,I/R조화Dex조AI화심기MDA함량승고,SOD활성강저,I/R조Bcl-2/Bax비치강저,Dex조Bcl-2/Bax비치승고(P<0.05);여 I/R조상비,Dex조AI화심기MDA함량강저,SOD활성화Bcl-2/Bax비치승고(P<0.05).결론 우미탁미정가감경신결혈재관주유발대서심기손상,기궤제가능여억제심기세포조망화감경지질과양화반응유관.
Objective To evaluate the effect of dexmedetomidine on myocardial injury induced by renal ischemia-reperfusion (I/R) injury in rats.Methods Twenty-four male Wistar rats,weighing 280-300 g,were randomly divided into 3 groups (n =8 each):sham operation group (group S),group I/R and dexmedetomidine group (group Dex).Renal ischemia was induced by occlusion of bilateral renal arteries for 45 min followed by reperfusion in I/R and Dex groups.At 20 min before ischemia,dexmedetomidine 50 μg/kg was injected intraperitoneally in group Dex,and the rest procedures were similar to those previously described in group I/R.The rats were sacrificed at 24 h of reperfusion and myocardial specimens were obtained for determination of malondialdehyde (MDA) content and superoxide dismutase (SOD) activity.The apoptosis in cardiomyocytes was examined by flow cytometry.Apoptosis index was calculated.The expression of Bcl-2 and Bax was detected by immunohistochemistry,and Bcl-2/Bax ratio was calculated.Results Compared with group S,apoptosis index and MDA content were significantly increased in I/R and Dex groups,Bcl-2/Bax ratio was decreased in group I/R,and Bcl-2/Bax ratio was increased in group Dex.Compared with group I/R,apoptosis index and MDA content were significantly decreased,and SOD activity and Bcl-2/Bax ratio were increased in group Dex.Conclusion Dexmedetomidine can attenuate myocardial injury induced by renal I/R in rats,and the mechanism may be related to inhibited apoptosis in cardiomyocytes and reduced lipid peroxidation.