中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
9期
1048-1050
,共3页
杨禄坤%白雪玲%肖笑雨%张荣凯
楊祿坤%白雪玲%肖笑雨%張榮凱
양록곤%백설령%초소우%장영개
哌啶类%再灌注损伤%四肢%肾
哌啶類%再灌註損傷%四肢%腎
고정류%재관주손상%사지%신
Piperidines%Reperfusion injury%Extremities%Kidney
目的 评价瑞芬太尼预先给药对大鼠肢体缺血再灌注致肾损伤的影响.方法 健康雄性SD大鼠27只,体重200 ～ 250 g,按照随机数字表法分为3组(n=9):假手术组(Sham组)、肢体缺血再灌注致肾损伤组(I/R组)和瑞芬太尼预先给药组(RPC组).I/R组和RPC组采用无创微动脉夹夹闭大鼠双侧股动静脉2h,再灌注24h的方法制备肢体缺血再灌注模型,Sham组仅暴露股动静脉,不阻断.RPC组缺血前尾静脉输注瑞芬太尼1.0 μg·kg-1·min-持续30 min,Sham组和I/R组输注等容量生理盐水.于再灌注24 h时抽取下腔静脉血样,断头处死大鼠取左肾.采用自动生化分析仪测定血清尿素氮(BUN)和肌酐(Cr)的浓度,ELISA法测定肾组织肿瘤坏死因子-α(TNF-α)和白细胞介素6(IL-6)的含量,TUNEL法检测肾组织细胞凋亡情况,计算凋亡指数(AI),HE染色光镜下观察肾组织病理学形态.结果 与Sham组比较,I/R组和RPC组血清BUN和Cr浓度、肾组织TNF-α和IL-6含量及AI均升高(P＜0.05),肾组织病理学损伤明显;与I/R组比较,RPC组血清BUN和Cr浓度、肾组织TNF-α和IL-6含量及AI均降低(P＜0.05),肾组织病理学损伤减轻.结论 瑞芬太尼预先给药可减轻肢体缺血再灌注致大鼠肾损伤,其机制可能与抑制肾组织炎症反应和细胞凋亡有关.
目的 評價瑞芬太尼預先給藥對大鼠肢體缺血再灌註緻腎損傷的影響.方法 健康雄性SD大鼠27隻,體重200 ～ 250 g,按照隨機數字錶法分為3組(n=9):假手術組(Sham組)、肢體缺血再灌註緻腎損傷組(I/R組)和瑞芬太尼預先給藥組(RPC組).I/R組和RPC組採用無創微動脈夾夾閉大鼠雙側股動靜脈2h,再灌註24h的方法製備肢體缺血再灌註模型,Sham組僅暴露股動靜脈,不阻斷.RPC組缺血前尾靜脈輸註瑞芬太尼1.0 μg·kg-1·min-持續30 min,Sham組和I/R組輸註等容量生理鹽水.于再灌註24 h時抽取下腔靜脈血樣,斷頭處死大鼠取左腎.採用自動生化分析儀測定血清尿素氮(BUN)和肌酐(Cr)的濃度,ELISA法測定腎組織腫瘤壞死因子-α(TNF-α)和白細胞介素6(IL-6)的含量,TUNEL法檢測腎組織細胞凋亡情況,計算凋亡指數(AI),HE染色光鏡下觀察腎組織病理學形態.結果 與Sham組比較,I/R組和RPC組血清BUN和Cr濃度、腎組織TNF-α和IL-6含量及AI均升高(P＜0.05),腎組織病理學損傷明顯;與I/R組比較,RPC組血清BUN和Cr濃度、腎組織TNF-α和IL-6含量及AI均降低(P＜0.05),腎組織病理學損傷減輕.結論 瑞芬太尼預先給藥可減輕肢體缺血再灌註緻大鼠腎損傷,其機製可能與抑製腎組織炎癥反應和細胞凋亡有關.
목적 평개서분태니예선급약대대서지체결혈재관주치신손상적영향.방법 건강웅성SD대서27지,체중200 ～ 250 g,안조수궤수자표법분위3조(n=9):가수술조(Sham조)、지체결혈재관주치신손상조(I/R조)화서분태니예선급약조(RPC조).I/R조화RPC조채용무창미동맥협협폐대서쌍측고동정맥2h,재관주24h적방법제비지체결혈재관주모형,Sham조부폭로고동정맥,불조단.RPC조결혈전미정맥수주서분태니1.0 μg·kg-1·min-지속30 min,Sham조화I/R조수주등용량생리염수.우재관주24 h시추취하강정맥혈양,단두처사대서취좌신.채용자동생화분석의측정혈청뇨소담(BUN)화기항(Cr)적농도,ELISA법측정신조직종류배사인자-α(TNF-α)화백세포개소6(IL-6)적함량,TUNEL법검측신조직세포조망정황,계산조망지수(AI),HE염색광경하관찰신조직병이학형태.결과 여Sham조비교,I/R조화RPC조혈청BUN화Cr농도、신조직TNF-α화IL-6함량급AI균승고(P＜0.05),신조직병이학손상명현;여I/R조비교,RPC조혈청BUN화Cr농도、신조직TNF-α화IL-6함량급AI균강저(P＜0.05),신조직병이학손상감경.결론 서분태니예선급약가감경지체결혈재관주치대서신손상,기궤제가능여억제신조직염증반응화세포조망유관.
Objective To evaluate the effects of remifentanil preconditioning on the renal injury induced by limb ischemia-reperfusion (I/R) in rats.Methods Twenty-seven healthy male Sprague-Dawley rats,weighing 200-250 g,were randomly assigned into 3 groups (n =9 each):sham operation group (Sham group),I/R group and remifentanil preconditioning group (RPC group).Limb ischemia was induced by clamping bilateral femoral arteries and veins for 2 h,followed by 24 h reperfusion in I/R and RPC groups.Remifentanil 1.0 μg· kg-1 · min-1 was infused via the caudal vein for 30 min before ischemia in RPC group,while the equal volume of normal saline was given in Sham and I/R groups.Blood sample was taken from the inferior vena cava at 24 h of reperfusion to measure blood urea nitrogen (BUN) and creatinine (Cr) concentrations in serum.The rats were sacrificed and the left kidney was removed for determination of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) contents,cell apoptosis and microscopic examination of pathological changes.Apoptosis index was calculated.Results Apoptosis index and concentrations of BUN and Cr in serum,TNF-α and IL-6 contents,and apoptosis index were significantly higher,and the pathological changes were more severe in I/R and RPC groups than in Sham group.Compared with group I/R,concentrations of BUN and Cr in serum,TNF-α and IL-6 contents,and apoptosis index were significantly decreased,and the pathological changes were attenuated in RPC group.Conclusion Remifentanil preconditioning can attenuate renal injury induced by limb I/R in rats,and inhibition of inflammatory responses and cell apoptosis in kidney may be involved in the mechanism.
