CAJ | 학술논문

目的评价孕早期长时间异丙酚麻醉对子代大鼠认知功能的影响.方法清洁级孕5～7d健康SD大鼠120只,体重280～320 g,采用随机数字表法,将其分为4组(n=30):对照组(C组)、静脉输注异丙酚2、4、8h组(P2组、P4组和P8组).P2组、P4组和P8组静脉注射异丙酚20 mg/kg后,以20 mg·kg-·h-1的速率分别静脉输注2、4和8h,C组静脉输注生理盐水2ml/kg.于子代大鼠出生后30、31、32、33、34、35、36 d时采用Morris水迷宫实验测定子代大鼠认知功能,水迷宫实验结束后,取子代大鼠海马组织,光镜和电镜下观察病理学结果.结果与C组比较,P4组和P8组子代大鼠逃避潜伏期延长,穿越原平台次数减少,原平台所在象限停留时间缩短(P＜0.05),P2组上述指标差异无统计学意义(P＞0.05).C组和P2组子代大鼠海马组织病理学结果未见异常,而P4组和P8组海马组织病理学损伤明显.结论孕早期长时间异丙酚麻醉可导致子代大鼠认知功能障碍,其机制与海马组织损伤有关.
목적 평개잉조기장시간이병분마취대자대대서인지공능적영향.방법 청길급잉5～7d건강SD대서120지,체중280～320 g,채용수궤수자표법,장기분위4조(n=30):대조조(C조)、정맥수주이병분2、4、8h조(P2조、P4조화P8조).P2조、P4조화P8조정맥주사이병분20 mg/kg후,이20 mg·kg-·h-1적속솔분별정맥수주2、4화8h,C조정맥수주생리염수2ml/kg.우자대대서출생후30、31、32、33、34、35、36 d시채용Morris수미궁실험측정자대대서인지공능,수미궁실험결속후,취자대대서해마조직,광경화전경하관찰병이학결과.결과 여C조비교,P4조화P8조자대대서도피잠복기연장,천월원평태차수감소,원평태소재상한정류시간축단(P＜0.05),P2조상술지표차이무통계학의의(P＞0.05).C조화P2조자대대서해마조직병이학결과미견이상,이P4조화P8조해마조직병이학손상명현.결론 잉조기장시간이병분마취가도치자대대서인지공능장애,기궤제여해마조직손상유관.
Objective To evaluate the effects of prolonged anesthesia with propofol during the early pregnancy on cognitive function of offspring rats.Methods One hundred and twenty female Sprague-Dawley rats at 5-7 days of gestation,weighing 280-320 g,were randomly divided into 4 groups (n =30 each) using a random number table:control group (group C),and propofol 2,4 and 8 h groups (P2,P4,P8 groups).In P2,P4,P8 groups,after propofol 20 mg/kg was injected intravenously,propofol was infused at 20 mg· kg-1 · h-1 for 2,4 and 8 h,respectively.In group C,normal saline 2 ml/kg was infused intravenously.At 30,31,32,33,34,35 and 36 days after birth,Morris Water maze was performed to evaluate the learning and memory abilities of offspring rats.At the end of Morris water maze test,the hippocampus of offspring rats was removed for microscopic examination of pathological changes with light and electron microscope.Results Compared with group C,the escape latencywas significantly prolonged,the frequency of crossing the original platform was decreased,and the time of staying at the original platform quadrant was shortened in p4 and P8 groups,and no significant changes were found in the indices mentioned above in group P2.The pathological changes of hippocampi were not found in C and P2 groups,while the pathological changes were obvious in P4 and P8 groups.Conclusion Prolonged anesthesia with propofol during the early pregnancy can induce cognitive dysfunction of offspring rats and the mechanism is related to the damage to hippocampal tissues.