CAJ | 학술논문

目的评价右美托咪定对大鼠肺缺血再灌注损伤的影响.方法健康SPF级雄性Wistar大鼠96只,体重250～350 g,8～ 12周龄,采用随机数字表法,将其分为4组(n=24):假手术组(S组)、肺缺血再灌注组(I/R组)、右美托咪定低剂量组(DL组)和右美托咪定高剂量组(DH组).DL组和DH组分别腹腔注射右美托咪定100和500μg· kg-1·d-1,1次/d,连续2d,S组和I/R组给予等容量生理盐水.于第2天给药后30 min采用夹闭左肺门45 min时开放的方法制备肺缺血再灌注损伤模型.分别于缺血45 min、再灌注60 min和120 min时处死6只大鼠,取肺组织,检测TNF-α含量和髓过氧化物酶(MPO)活性,于再灌注120 min时取肺组织,确定损伤肺泡百分比,另取6只大鼠,进行支气管肺泡灌洗,测定支气管肺泡灌洗液(BALF)总蛋白浓度.结果与S组比较,I/R组、DL组和DH组肺组织TNF-α含量、MPO活性、损伤肺泡百分比和BALF总蛋白浓度升高(P＜0.05);与I/R组比较,DL组和DH组肺组织TNF-α含量、MPO活性、损伤肺泡百分比和BALF总蛋白浓度降低(P＜0.05).结论右美托咪定可减轻大鼠肺缺血再灌注损伤,其机制与抑制炎性反应有关.
목적 평개우미탁미정대대서폐결혈재관주손상적영향.방법 건강SPF급웅성Wistar대서96지,체중250～350 g,8～ 12주령,채용수궤수자표법,장기분위4조(n=24):가수술조(S조)、폐결혈재관주조(I/R조)、우미탁미정저제량조(DL조)화우미탁미정고제량조(DH조).DL조화DH조분별복강주사우미탁미정100화500μg· kg-1·d-1,1차/d,련속2d,S조화I/R조급여등용량생리염수.우제2천급약후30 min채용협폐좌폐문45 min시개방적방법제비폐결혈재관주손상모형.분별우결혈45 min、재관주60 min화120 min시처사6지대서,취폐조직,검측TNF-α함량화수과양화물매(MPO)활성,우재관주120 min시취폐조직,학정손상폐포백분비,령취6지대서,진행지기관폐포관세,측정지기관폐포관세액(BALF)총단백농도.결과 여S조비교,I/R조、DL조화DH조폐조직TNF-α함량、MPO활성、손상폐포백분비화BALF총단백농도승고(P＜0.05);여I/R조비교,DL조화DH조폐조직TNF-α함량、MPO활성、손상폐포백분비화BALF총단백농도강저(P＜0.05).결론 우미탁미정가감경대서폐결혈재관주손상,기궤제여억제염성반응유관.
Objective To evaluate the effects of dexmedetomidine on lung ischemia-reperfusion (I/R) injury in rats.Methods Ninety-six healthy SPF male Wistar rats,weighing 250-350 g,aged 8-12 weeks,were randomly divided into 4 groups (n =24 each):sham operation group (S group),I/R group,low dose dexmedetomidine group (DL group) and high dose dexmedetomidine group (DH group).In DL and DH groups,dexmedetomidine 100 and 500 μg· kg-1 · d-1 were injected intraperitoneally once a day for 2 consecutive days,while the equal volume of normal saline was given in S and I/R groups.Lung·I/R was induced by clamping the left hilum of lung for 45 min followed by reperfusion at 30 min after administration on 2nd day.At 45 min of ischemia,and 60 and 120 min of reperfusion,6 rats were sacrificed,and lungswere removed for determination of TNF-α (tumor necrosis factor-a) content and myeloperoxidase (MPO) activity in lung tissues.The percentage of damaged alveolar in lung tissues was detected at 120 min of reperfusion.Another 6 rats were lavaged and the broncho-alveolar lavage fluid (BALF) was colleted for determination of the total protein concentrations.Results Compared with S group,the TNF-α content,MPO activity,percentage of damaged alveolar,and total protein concentrations in BALF were significantly increased in I/R,DL and DH groups.Compared with I/R group,the TNF-α content,MPO activity,percentage of damaged alveolar,and total protein concentrations in BALF were significantly decreased in DL and DH groups.Conclusion Dexmedetomidine can alleviate the lung I/R injury in rats,and the mechanism is related to inhibiton of the inflammatory responses.