中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
9期
1082-1085
,共4页
杨雪%吴昊澎%彭捷%熊家祥%陆建华
楊雪%吳昊澎%彭捷%熊傢祥%陸建華
양설%오호팽%팽첩%웅가상%륙건화
基因疗法%转染%RNA,小分子干扰%受体,N-甲基-D-天冬氨酸%神经痛%注射,腹腔内
基因療法%轉染%RNA,小分子榦擾%受體,N-甲基-D-天鼕氨痠%神經痛%註射,腹腔內
기인요법%전염%RNA,소분자간우%수체,N-갑기-D-천동안산%신경통%주사,복강내
Gene therapy%Transfection%RNA,small interfering%Receptors,N-methyl-D-aspartate%Neuralgia%Injections,intraperitoneal
目的 评价腹腔注射水溶性脂聚体(WSLP)/含2B亚基的N-甲基-D-天冬氨酸受体(NR2B) siRNA复合物对大鼠神经病理性痛的影响.方法 健康雄性SD大鼠84只,6周龄,体重180～ 200 g,采用随机数字表法分为7组(n=12):空白对照组(C组)、假手术组(S组)、神经病理性痛组(NP组)、WSLP/NR2B siRNA组(siWSLP组)、WSLP/阴性对照siRNA组(ncWSLP组)、PE[/NR2B siRNA组(PEI组)和WSLP组.C组不给予任何处理,S组仅暴露L5脊神经但不结扎,NP组、siWSLP组、ncWSLP组、PEI组及WSLP组采用结扎L5脊神经的方法制备大鼠神经病理性痛模型,于模型制备后第10天分别单次腹腔注射WSLP/NR2B siRNA、WSLP/阴性对照siRNA、PEI/NR2B siRNA及WSLP复合物2ml.于术前1d、术后7d及腹腔给药后3、7、14及21 d时随机取6只大鼠测定机械缩足反应阈(MWT)和热缩足反应潜伏期(TWL);于腹腔注射后3d余6只大鼠处死后取脊髓,采用PCR法检测脊髓NR2B mRNA表达水平,采用Western blot法检测脊髓NR2B蛋白的表达水平.结果 与C组比较,其余组术后7d及腹腔给药后3、7和21 d时MWT降低,TWL缩短,腹腔给药后3d时脊髓NR2B mRNA及其蛋白的表达上调(P＜0.01);与NP组比较,siWSLP组腹腔给药后3和7d时MWT升高,TWL延长,腹腔给药后3d时脊髓NR2B mRNA及其蛋白的表达下调(P＜0.01),PEI组、ncWSLP组、WSLP组及S组各指标差异无统计学意义(P＞0.05).结论 腹腔注射WSLP/NR2B siRNA复合物可有效减轻大鼠神经病理性痛.
目的 評價腹腔註射水溶性脂聚體(WSLP)/含2B亞基的N-甲基-D-天鼕氨痠受體(NR2B) siRNA複閤物對大鼠神經病理性痛的影響.方法 健康雄性SD大鼠84隻,6週齡,體重180～ 200 g,採用隨機數字錶法分為7組(n=12):空白對照組(C組)、假手術組(S組)、神經病理性痛組(NP組)、WSLP/NR2B siRNA組(siWSLP組)、WSLP/陰性對照siRNA組(ncWSLP組)、PE[/NR2B siRNA組(PEI組)和WSLP組.C組不給予任何處理,S組僅暴露L5脊神經但不結扎,NP組、siWSLP組、ncWSLP組、PEI組及WSLP組採用結扎L5脊神經的方法製備大鼠神經病理性痛模型,于模型製備後第10天分彆單次腹腔註射WSLP/NR2B siRNA、WSLP/陰性對照siRNA、PEI/NR2B siRNA及WSLP複閤物2ml.于術前1d、術後7d及腹腔給藥後3、7、14及21 d時隨機取6隻大鼠測定機械縮足反應閾(MWT)和熱縮足反應潛伏期(TWL);于腹腔註射後3d餘6隻大鼠處死後取脊髓,採用PCR法檢測脊髓NR2B mRNA錶達水平,採用Western blot法檢測脊髓NR2B蛋白的錶達水平.結果 與C組比較,其餘組術後7d及腹腔給藥後3、7和21 d時MWT降低,TWL縮短,腹腔給藥後3d時脊髓NR2B mRNA及其蛋白的錶達上調(P＜0.01);與NP組比較,siWSLP組腹腔給藥後3和7d時MWT升高,TWL延長,腹腔給藥後3d時脊髓NR2B mRNA及其蛋白的錶達下調(P＜0.01),PEI組、ncWSLP組、WSLP組及S組各指標差異無統計學意義(P＞0.05).結論 腹腔註射WSLP/NR2B siRNA複閤物可有效減輕大鼠神經病理性痛.
목적 평개복강주사수용성지취체(WSLP)/함2B아기적N-갑기-D-천동안산수체(NR2B) siRNA복합물대대서신경병이성통적영향.방법 건강웅성SD대서84지,6주령,체중180～ 200 g,채용수궤수자표법분위7조(n=12):공백대조조(C조)、가수술조(S조)、신경병이성통조(NP조)、WSLP/NR2B siRNA조(siWSLP조)、WSLP/음성대조siRNA조(ncWSLP조)、PE[/NR2B siRNA조(PEI조)화WSLP조.C조불급여임하처리,S조부폭로L5척신경단불결찰,NP조、siWSLP조、ncWSLP조、PEI조급WSLP조채용결찰L5척신경적방법제비대서신경병이성통모형,우모형제비후제10천분별단차복강주사WSLP/NR2B siRNA、WSLP/음성대조siRNA、PEI/NR2B siRNA급WSLP복합물2ml.우술전1d、술후7d급복강급약후3、7、14급21 d시수궤취6지대서측정궤계축족반응역(MWT)화열축족반응잠복기(TWL);우복강주사후3d여6지대서처사후취척수,채용PCR법검측척수NR2B mRNA표체수평,채용Western blot법검측척수NR2B단백적표체수평.결과 여C조비교,기여조술후7d급복강급약후3、7화21 d시MWT강저,TWL축단,복강급약후3d시척수NR2B mRNA급기단백적표체상조(P＜0.01);여NP조비교,siWSLP조복강급약후3화7d시MWT승고,TWL연장,복강급약후3d시척수NR2B mRNA급기단백적표체하조(P＜0.01),PEI조、ncWSLP조、WSLP조급S조각지표차이무통계학의의(P＞0.05).결론 복강주사WSLP/NR2B siRNA복합물가유효감경대서신경병이성통.
Objective To evaluate the effect of intraperitoneal water soluble lipopolymer (WSLP)/ N-methyl-D-aspartate receptor subunit 2B (NR2B) siRNA compound on the neuropathic pain (NP) in rats.Methods Eighty-four healthy male Sprague-Dawley rats,aged 6 weeks,weighing 180-220 g,were randomly divided into 7 groups (n =12 each) using a random number table:control group (group C),sham operation group (group S),NP group,WSLP/NR2B siRNA group (siWSLP group),WSLP/negative control siRNA group (ncWSLP group),PEI/NR2B siRNA group (PEI group) and WSLP group (WSLP group).NP was produced by ligation of the left L5 spinal nerve.In group S,the left L5 spinal nerve was only exposed,but not ligated.In group C,the rats underwent no treatment.Groups siWSLP,ncWSLP,PEI and WSLP received single intraperitoneal injection of WSLP/NR2B siRNA,WSLP/negative control siRNA,PEI/NR2B siRNA and WSLP compound 2 ml,respectively,at 10 days after NP.At 1 day before operation,7 days after operation,and 3,7,14 and 21 days after intraperitoneal injection,6 rats in each group were chosen randomly to measure mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL).At 3 days after intraperitoneal injection,the left 6 rats in each group were sacrificed and the spinal cord was removed for detection of NP2B mRNA expression (using PCR) and NR2B expression (by Western blot).Results Compared with group C,MWT was significantly decreased,and TWL was shortened on 7 days after operation and 3,7 and 21 days after intraperitoneal injection,and the expression of NR2B mRNA and protein was down-regulated on 3 days after administration in the other groups.Compared with group NP,MWT was significantly increased,and TWL was prolonged on day 3 and 7 after intraperitoneal injection,and the expression of NR2B mRNA and protein was down-regulated on day 3 after administration in siWSLP group.Conclusion Intraperitoneal WSLP/NR2B siRNA compound can effectively relieve the NP in rats.
