CAJ | 학술논문

目的评价脊髓背根神经节神经元瞬时受体电位离子通道1(TRPA1)在大鼠糖尿病神经痛形成中的作用.方法糖尿病神经痛模型制备成功的SD大鼠24只,采用随机数字表法分为3组(n=8):糖尿病神经痛组(DNP组)、TRPA1 siRNA组(siRNA组)和TRPA1-阴性siRNA组(NC组).另取血糖正常的SD大鼠8只,作为正常对照组(C组).siRNA组鞘内注射TRPA1 siRNA 45 μL,NC组鞘内注射TRPA1-阴性siRNA 45 μL,DNP组和C组鞘内注射生理盐水45μL.各组于鞘内注药后第2天,取L4-6背根神经节,采用RT-PCR法测定神经元TRPA1 mRNA的表达,于鞘内注药后第7、14、21和28天(T1-4)时测定机械缩足反应阈(MWT).结果与DNP组比较,siRNA组和C组脊髓背根神经节神经元TRPA1 mRNA表达下调(P＜0.05);与siRNA组比较,NC组脊髓背根神经节神经元TRPA1 mRNA表达上调(P＜0.05);与NC组比较,C组脊髓背根神经节神经元TRPA1 mRNA表达下调(P＜0.05).与DNP组比较,siRNA组T1.2时、NC组T1-3时MWT降低,C组T1-4时MWT升高(P＜0.05);与siRNA组比较,C组T1-4时MWT升高(P＜0.05);与NC比较,C组T1-4时MWT升高(P＜0.05).结论脊髓背根神经节神经元TRPA1参与大鼠糖尿病神经痛的形成.
목적 평개척수배근신경절신경원순시수체전위리자통도1(TRPA1)재대서당뇨병신경통형성중적작용.방법 당뇨병신경통모형제비성공적SD대서24지,채용수궤수자표법분위3조(n=8):당뇨병신경통조(DNP조)、TRPA1 siRNA조(siRNA조)화TRPA1-음성siRNA조(NC조).령취혈당정상적SD대서8지,작위정상대조조(C조).siRNA조초내주사TRPA1 siRNA 45 μL,NC조초내주사TRPA1-음성siRNA 45 μL,DNP조화C조초내주사생리염수45μL.각조우초내주약후제2천,취L4-6배근신경절,채용RT-PCR법측정신경원TRPA1 mRNA적표체,우초내주약후제7、14、21화28천(T1-4)시측정궤계축족반응역(MWT).결과 여DNP조비교,siRNA조화C조척수배근신경절신경원TRPA1 mRNA표체하조(P＜0.05);여siRNA조비교,NC조척수배근신경절신경원TRPA1 mRNA표체상조(P＜0.05);여NC조비교,C조척수배근신경절신경원TRPA1 mRNA표체하조(P＜0.05).여DNP조비교,siRNA조T1.2시、NC조T1-3시MWT강저,C조T1-4시MWT승고(P＜0.05);여siRNA조비교,C조T1-4시MWT승고(P＜0.05);여NC비교,C조T1-4시MWT승고(P＜0.05).결론 척수배근신경절신경원TRPA1삼여대서당뇨병신경통적형성.
Objective To evaluate the role of transient receptor potential ankyrin 1 (TRPA1) in the dorsal root ganglion neurons in the development of diabetic neuropathic pain (DNP) in rats.Methods Twenty-four Sprague-Dawley rats with DNP were randomly divided into 3 groups (n-=8 each) using a random number table:DNP group,TRPA1-specific siRNA group (siRNA group) and TRPA1-negative siRNA group (NC group).Another 8 Sprague-Dawley rats with normal blood glucose served as control group (C group).In siRNA group,TRPA1-specific siRNA 45 μl was injected intrathecally.In NC group,TRPA1-negative siRNA 45 μl was injected intrathecally.In DNP and C groups,normal saline 45 μl was injected intrathecally.On 2nd day after intrathecal administration,the lumbar segment (L4-6) of the dorsal root ganglions was removed for determination of the expression of TRPA1 mRNA.On 7,14,21 and 28 days after intrathecal administration (T1-4),MWT was measured.Results Compared with DNP group,TRPA1 mRNA expression was down-regulated in siRNA and C groups.Compared with DNP group,and MWT was significantly decreased at T1.2 in siRNA group,MWT was decreased at T1-3 in NC group,MWT was increased at T1-4 in group C.Compared with siRNA group,MWT was significantly increased at T1-4 in group C.MWT was significantly higher at T1~ in group C than in NC group.Conclusion TRPA1 in the dorsal root ganglion neurons is involved in the development of DNP in rats.