CAJ | 학술논문

目的评价切口痛大鼠十二指肠胃动素水平的变化.方法 SPF级健康雄性SD大鼠84只,6～8周龄,体重180 ～ 220 g,采用随机数字表法,将其分为2组(n=42):对照组(C组)和切口痛组(P组).行右足跖肌切口以制备切口痛模型.2组取6只大鼠,于术前24 h(T0)、术后1 h(T1)、6h(T2)、24 h(T3)、48 h(T4)、72 h(T5)时测定机械缩足反应阈(MWT)与热缩足反应潜伏期(TWL),并于上述各时点分别取6只大鼠,处死,取十二指肠粘膜组织,采用ELISA法测定胃动素水平.P组各时点十二指肠胃动素含量与痛阈之间进行Pearson相关分析.结果与C组比较,P组T1-4时MWT降低,TWL缩短,十二指肠胃动素含量升高(P＜0.05),T0.5时差异无统计学意义(P＞0.05).P组十二指肠胃动素水平与MWT和TWL均呈负相关,r分别为-0.8 910和-0.8 463(P＜0.01).结论切口痛可促进大鼠十二指肠胃动素分泌.
목적 평개절구통대서십이지장위동소수평적변화.방법 SPF급건강웅성SD대서84지,6～8주령,체중180 ～ 220 g,채용수궤수자표법,장기분위2조(n=42):대조조(C조)화절구통조(P조).행우족척기절구이제비절구통모형.2조취6지대서,우술전24 h(T0)、술후1 h(T1)、6h(T2)、24 h(T3)、48 h(T4)、72 h(T5)시측정궤계축족반응역(MWT)여열축족반응잠복기(TWL),병우상술각시점분별취6지대서,처사,취십이지장점막조직,채용ELISA법측정위동소수평.P조각시점십이지장위동소함량여통역지간진행Pearson상관분석.결과 여C조비교,P조T1-4시MWT강저,TWL축단,십이지장위동소함량승고(P＜0.05),T0.5시차이무통계학의의(P＞0.05).P조십이지장위동소수평여MWT화TWL균정부상관,r분별위-0.8 910화-0.8 463(P＜0.01).결론 절구통가촉진대서십이지장위동소분비.
Objective To evaluate the changes in the levels of motilin in the duodenum in a rat model of incisional pain.Methods Eighty-four healthy male Sprague-Dawley rats,aged 6-8 months,weighing 180-220 g,were randomized into 2 groups (n =42 each) using a random number table:control group (group C) and incisional pain group (group P).The animals were anesthetized with sevoflurane.In group P,a 1 cm long incision was made in the plantar surface of right hindpaw.Six rats were chosen from each group and mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 24 h before operation (T0) and 1,6,24,48 and 72 h after operation (T1-5).Six rats were chosen from each group at T0-5 and sacrificed and the duodenal mucosal tissue was prepared for measurement of motilin levels by ELISA.Pearson linear correlate analysis was performed between the motilin level and pain threshold at each time point in group P.Results Compared with group C,MWT was significantly decreased,TWL was shortened,and motilin levels were significantly increased at T1-4,and no significant change was found at T0 and T5 in group P.The motilin levels were negatively correlated with MWT (r =-0.8 910) and TWL (r =-0.8 463) in group P.Conclusion Incisional pain can promote the secretion of motilin in the duodenum.