CAJ | 학술논문

目的评价瑞芬太尼后处理对大鼠心肌缺血再灌注损伤时水通道蛋白1(AQP-1)表达的影响.方法健康雄性SD大鼠24只,体重250 ～ 300 g,采用随机数字表法将其分为3组(n=8):假手术组(S组)、缺血再灌注组(I/R组)和瑞芬太尼后处理组(RP组).采用结扎左冠状动脉前降支45 min再灌注24 h的方法建立心肌缺血再灌注损伤模型.RP组于再灌注前10 min开始静脉输注瑞芬太尼10 μg·kg-·min-1,经10 min输注完毕,S组和I/R组给予等容量生理盐水.于再灌注结束时处死大鼠,取左心室组织,光镜下观察心肌组织病理学结果,采用实时荧光定量PCR法测定缺血区心肌组织AQP-1mRNA表达,采用Western blot法测定缺血区心肌组织AQP-1蛋白表达,计算心肌含水率.结果与S组比较,其余2组缺血区心肌组织含水率增加,I/R组心肌组织AQP-1 mRNA和蛋白表达上调(P＜0.05),RP组心肌组织AQP-1 mRNA和蛋白表达比较差异无统计学意义(P＞0.05);与I/R组比较,RP组心肌组织含水率减少,心肌组织AQP-1 mRNA和蛋白表达下调(P＜0.05).结论瑞芬太尼后处理可能通过下调心肌组织AQP-1表达减轻大鼠心肌缺血再灌注损伤.
목적 평개서분태니후처리대대서심기결혈재관주손상시수통도단백1(AQP-1)표체적영향.방법 건강웅성SD대서24지,체중250 ～ 300 g,채용수궤수자표법장기분위3조(n=8):가수술조(S조)、결혈재관주조(I/R조)화서분태니후처리조(RP조).채용결찰좌관상동맥전강지45 min재관주24 h적방법건립심기결혈재관주손상모형.RP조우재관주전10 min개시정맥수주서분태니10 μg·kg-·min-1,경10 min수주완필,S조화I/R조급여등용량생리염수.우재관주결속시처사대서,취좌심실조직,광경하관찰심기조직병이학결과,채용실시형광정량PCR법측정결혈구심기조직AQP-1mRNA표체,채용Western blot법측정결혈구심기조직AQP-1단백표체,계산심기함수솔.결과 여S조비교,기여2조결혈구심기조직함수솔증가,I/R조심기조직AQP-1 mRNA화단백표체상조(P＜0.05),RP조심기조직AQP-1 mRNA화단백표체비교차이무통계학의의(P＞0.05);여I/R조비교,RP조심기조직함수솔감소,심기조직AQP-1 mRNA화단백표체하조(P＜0.05).결론 서분태니후처리가능통과하조심기조직AQP-1표체감경대서심기결혈재관주손상.
Objective To evaluate the effects of remifentanil post-conditioning on aquaporin-1 (AQP-1) expression during myocardial ischemia-reperfusion (I/R) injury in rats.Methods Twenty-four male.SpragueDawley rats,weighing 250-300 g,were randomly divided into 3 groups (n =8 each) using a random number table:sham operation group (group S),group I/R,and remifentanil post-conditioning group (group RP).Myocardial I/R was induced by 45 min occlusion of left anterior descending branch of coronary artery followed by 24 h reperfusion.Remifentanil 10 μg· kg-1· min-1 was infused over 10 min starting from 10 min before reperfusion in group RP,while the equal volume of normal saline was given instead in S and I/R groups.At the end of reperfusion,all the rats were sacrificed and their myocardial specimens from left ventricles were obtained for microscopic examination of thepathological changes and for determination of AQP-1 mRNA (using real-time fluorescent quantitative PC R) and AQP-1 protein (by Western blot) expression in the ischemic area and myocardial water content.Results Compared with S group,myocardial water content was significantly increased in the other two groups,AQP-1 mRNA and protein expression was up-regulated in group I/R,and no significant change was found in AQP-1 mRNA and protein expression in RP group.Compared with I/R group,myocardial water content was significantly reduced,and AQP-1 mRNA and protein expression was down-regulated in RP group.Conclusion Remifentanil post-conditioning reduces myocardial I/R injury possibly through down-regulating AQP-1 expression in myocardial tissues of rats.