CAJ | 학술논문

目的评价浅低温对全脑缺血再灌注大鼠海马微小RNA 210(miR-210)表达的影响.方法健康雄性SD大鼠60只,9～ 10周龄,体重280 ～ 320 g,采用随机数字表法将其分为3组(n=20):假手术组(S组)、缺血再灌注组(I/R组)和浅低温组(H组).采用经食管心脏起搏诱发室颤以及心肺复苏建立大鼠全脑缺血再灌注模型.H组于再灌注即刻乙醇涂擦体表,10 min内将直肠温度降至32 ～ 34℃,平稳维持6h,其余组用加热毯维持直肠温度36～37℃.于再灌注24h时处死取双侧海马组织,采用实时定量PCR法检测海马miR-210的表达水平,采用TUNEL法测定海马细胞凋亡情况.结果与S组比较,I/R组海马miR-210表达上调,细胞凋亡率升高(P＜0.05);与I/R组比较,H组海马miR-210表达下调,细胞凋亡率降低(P＜0.05).结论浅低温的脑复苏作用可能与通过下调海马miR-210表达,进而减少海马细胞凋亡有关.
목적 평개천저온대전뇌결혈재관주대서해마미소RNA 210(miR-210)표체적영향.방법 건강웅성SD대서60지,9～ 10주령,체중280 ～ 320 g,채용수궤수자표법장기분위3조(n=20):가수술조(S조)、결혈재관주조(I/R조)화천저온조(H조).채용경식관심장기박유발실전이급심폐복소건립대서전뇌결혈재관주모형.H조우재관주즉각을순도찰체표,10 min내장직장온도강지32 ～ 34℃,평은유지6h,기여조용가열담유지직장온도36～37℃.우재관주24h시처사취쌍측해마조직,채용실시정량PCR법검측해마miR-210적표체수평,채용TUNEL법측정해마세포조망정황.결과 여S조비교,I/R조해마miR-210표체상조,세포조망솔승고(P＜0.05);여I/R조비교,H조해마miR-210표체하조,세포조망솔강저(P＜0.05).결론 천저온적뇌복소작용가능여통과하조해마miR-210표체,진이감소해마세포조망유관.
Objective To evaluate the effect of mild hypothermia on expression of hippocampal microRNA-210 (miR-210) during global cerebral ischemia-reperfusion (I/R) in rats.Methods Sixty healthy male Sprague-Dawley rats,aged 9-10 weeks,weighing 280-320g,were randomly divided into 3 groups (n =20 each):sham operation group (group S),group I/R,and mild hypothermia group (group H).Ventricular fibrillation (VF) was induced with transoesophageal cardiac pacing followed by cardiopulmonary resuscitation to establish the global cerebral I/R model.In group H,the rectal temperature was cooled down to 32-34 ℃ by rubbing body surface with ethanol within 10 min starting from onset of reperfusion and maintained at this level for 6 h.In the other groups,the rectal temperature was maintained at 36-37 ℃ with a heating blanket.Rats were sacrificed at 24 h of reperfusion,and then the bilateral hippocampi were removed for detection of miR-210 expression (using RT-PCR) and apoptosis (by TUNEL).Results Compared with group S,the expression of miR-210 was up-regulated,and apoptosis rate was increased in group I/R.Compared with group I/R,the expression of miR-210 was down-regulated,and apoptosis rate was decreased in group H.Conclusion Mild hypothermia playes a role in cerebral resuscitation possibly by down-regulating miR-210 expression and reducing apoptosis in hippocampal cells of rats.