中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
10期
1207-1210
,共4页
史小婷%徐霞%曹红%李佳佳%吴绍胜%李军
史小婷%徐霞%曹紅%李佳佳%吳紹勝%李軍
사소정%서하%조홍%리가가%오소성%리군
姜黄素%糖尿病,2型%神经痛%脊髓%神经节,脊%晚期糖基化终末产物受体
薑黃素%糖尿病,2型%神經痛%脊髓%神經節,脊%晚期糖基化終末產物受體
강황소%당뇨병,2형%신경통%척수%신경절,척%만기당기화종말산물수체
Curcumin%Diabetes mellitus,type 2%Neuralgia%Spinal cord%Ganglia,spinal%Receptor for advanced glycation end-products
目的 评价姜黄素对2型糖尿病神经痛大鼠脊髓背角和背根神经节晚期糖基化终末产物受体(RAGE)表达的影响.方法 成年雄性SD大鼠,体重160～ 180 g,高脂高糖饲料喂养8周诱导胰岛素抵抗,然后腹腔注射链脲佐菌素(STZ)35 mg/kg,3d后血糖浓度≥16.7 mmol/L为2型糖尿病大鼠;14 d后测定机械缩足反应阈(MWT)和热缩足反应潜伏期(TWL),低于基础值80％的大鼠为2型糖尿病神经痛大鼠.取2型糖尿病神经痛大鼠81只,采用随机数字表法分为3组(n=27):糖尿病神经痛组(DNP组)、糖尿病神经痛+姜黄素组(DCur组)和糖尿病神经痛+溶剂组(DSC组).DCur组和DSC组于STZ注射后14 d分别腹腔注射姜黄素100 mg·kg-1·d-1和玉米油4 m1·kg-1·d-1,连续14 d.另取27只正常大鼠作为对照组(C组).分别于STZ注射前、注射后14 d及给予姜黄素3、7和14 d时时测定MWT和TWL.给予姜黄素3、7和14d测定痛阈后采用免疫组化法测定脊髓背角和背根神经节RAGE阳性细胞率,采用免疫印迹法测定脊髓背角和背根神经节RAGE表达.结果 与C组比较,DNP组STZ注射后14 d和给予姜黄素各时点MWT降低,TWL缩短,给予姜黄素各时点脊髓背角和背根神经节RAGE阳性细胞率升高,给予姜黄素各时点脊髓背角、给予姜黄素7和14 d时背根神经节RAGE表达上调(P＜0.05).与DNP组比较,DCur组给予姜黄素各时点MWT升高,TWL延长,给予姜黄素7和14 d时脊髓背角和背根神经节RAGE阳性细胞率降低,给予姜黄素各时点脊髓背角、给予姜黄素7和14 d时背根神经节RAGE表达下调(P＜0.05),DSC组上述各指标差异无统计学意义(P＞0.05).结论 姜黄素减轻大鼠2型糖尿病神经痛的机制可能与抑制脊髓背角和背根神经节RAGE表达上调有关.
目的 評價薑黃素對2型糖尿病神經痛大鼠脊髓揹角和揹根神經節晚期糖基化終末產物受體(RAGE)錶達的影響.方法 成年雄性SD大鼠,體重160～ 180 g,高脂高糖飼料餵養8週誘導胰島素牴抗,然後腹腔註射鏈脲佐菌素(STZ)35 mg/kg,3d後血糖濃度≥16.7 mmol/L為2型糖尿病大鼠;14 d後測定機械縮足反應閾(MWT)和熱縮足反應潛伏期(TWL),低于基礎值80％的大鼠為2型糖尿病神經痛大鼠.取2型糖尿病神經痛大鼠81隻,採用隨機數字錶法分為3組(n=27):糖尿病神經痛組(DNP組)、糖尿病神經痛+薑黃素組(DCur組)和糖尿病神經痛+溶劑組(DSC組).DCur組和DSC組于STZ註射後14 d分彆腹腔註射薑黃素100 mg·kg-1·d-1和玉米油4 m1·kg-1·d-1,連續14 d.另取27隻正常大鼠作為對照組(C組).分彆于STZ註射前、註射後14 d及給予薑黃素3、7和14 d時時測定MWT和TWL.給予薑黃素3、7和14d測定痛閾後採用免疫組化法測定脊髓揹角和揹根神經節RAGE暘性細胞率,採用免疫印跡法測定脊髓揹角和揹根神經節RAGE錶達.結果 與C組比較,DNP組STZ註射後14 d和給予薑黃素各時點MWT降低,TWL縮短,給予薑黃素各時點脊髓揹角和揹根神經節RAGE暘性細胞率升高,給予薑黃素各時點脊髓揹角、給予薑黃素7和14 d時揹根神經節RAGE錶達上調(P＜0.05).與DNP組比較,DCur組給予薑黃素各時點MWT升高,TWL延長,給予薑黃素7和14 d時脊髓揹角和揹根神經節RAGE暘性細胞率降低,給予薑黃素各時點脊髓揹角、給予薑黃素7和14 d時揹根神經節RAGE錶達下調(P＜0.05),DSC組上述各指標差異無統計學意義(P＞0.05).結論 薑黃素減輕大鼠2型糖尿病神經痛的機製可能與抑製脊髓揹角和揹根神經節RAGE錶達上調有關.
목적 평개강황소대2형당뇨병신경통대서척수배각화배근신경절만기당기화종말산물수체(RAGE)표체적영향.방법 성년웅성SD대서,체중160～ 180 g,고지고당사료위양8주유도이도소저항,연후복강주사련뇨좌균소(STZ)35 mg/kg,3d후혈당농도≥16.7 mmol/L위2형당뇨병대서;14 d후측정궤계축족반응역(MWT)화열축족반응잠복기(TWL),저우기출치80％적대서위2형당뇨병신경통대서.취2형당뇨병신경통대서81지,채용수궤수자표법분위3조(n=27):당뇨병신경통조(DNP조)、당뇨병신경통+강황소조(DCur조)화당뇨병신경통+용제조(DSC조).DCur조화DSC조우STZ주사후14 d분별복강주사강황소100 mg·kg-1·d-1화옥미유4 m1·kg-1·d-1,련속14 d.령취27지정상대서작위대조조(C조).분별우STZ주사전、주사후14 d급급여강황소3、7화14 d시시측정MWT화TWL.급여강황소3、7화14d측정통역후채용면역조화법측정척수배각화배근신경절RAGE양성세포솔,채용면역인적법측정척수배각화배근신경절RAGE표체.결과 여C조비교,DNP조STZ주사후14 d화급여강황소각시점MWT강저,TWL축단,급여강황소각시점척수배각화배근신경절RAGE양성세포솔승고,급여강황소각시점척수배각、급여강황소7화14 d시배근신경절RAGE표체상조(P＜0.05).여DNP조비교,DCur조급여강황소각시점MWT승고,TWL연장,급여강황소7화14 d시척수배각화배근신경절RAGE양성세포솔강저,급여강황소각시점척수배각、급여강황소7화14 d시배근신경절RAGE표체하조(P＜0.05),DSC조상술각지표차이무통계학의의(P＞0.05).결론 강황소감경대서2형당뇨병신경통적궤제가능여억제척수배각화배근신경절RAGE표체상조유관.
Objective To evaluate the effects of curcumin on the expression of receptor for advanced glycation end-products (RAGE) in the spinal dorsal horn and dorsal root ganglion (DRG) of the rats with type 2 diabetic neuropathic pain (DNP).Methods Male Sprague-Dawley rats,weighing 160-180 g,were used in this study.Type 2 diabetes mellitus was induced by high-fat and high-sucrose diet for 8 weeks and intraperitoneal streptozotocin (STZ) 35 mg/kg and confirmed by fasting blood glucose level ≥ 16.7 mmol/L 3 days later.Type 2 DNPwas confirmed by the mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) measured on day 14 after STZ administration less than 80％ of the baseline value.Eighty-one rats with type 2 DNP were randomly divided into 3 groups (n =27 each) using a random number table:DNP group,DNP + curcumin group (DCur group),and DNP+ solvent group (group DSC).In DCur and DSC groups,curcumin 100 mg· kg-1 · d-1 and corn oil 4 ml · kg-1 · d-1 were injected intraperitonally,respectively,for 14 consecutive days starting from the day 14 after STZ administration.Another 27 normal male Sprague-Dawley rats served as control group (group C) and were fed with normal forage.MWT and TWL were measured before STZ injection,at day 14 after STZ injection,and on 3,7 and 14 days after curcumin injection.RAGE positive cells were determined by immuno-histochemistry and the expression of RAGE by Western blot in the spinal dorsal horn and DRG after MWT and TWL were measured on 3,7 and 14 days after curcumin injection.Results Compared with group C,MWT was significantly decreased and TWL was shortened at 14 days after STZ injection and each time point after curcumin injection,the rate of RAGE positive cells in the spinal dorsal horn and DRG was increased at each time point after curcumin injection,and the expression of RAGE was up-regulated in the spinal dorsal horn at each time point after curcumin injection and in the DRG at 7 and 14 days after curcumin injection in group DNP.Compared with group DNP,MWT was significantly increased and TWL was prolonged at each time point after curcumin injection,the rate of RAGE positive cells in the spinal dorsal horn and DRG was significantly decreased at 7 and 14 days after curcumin injection,and the expression of RAGE was down-regulated in the spinal dorsal horn at each time point after curcumin injection and in the DRG at 7 and 14 days after curcumin injection in group DCur,and no significant change was found in the parameters mentioned above in group DSC.Conclusion The mechanism by which curcumin attenuates DNP may be related to inhibition of up-regulated expression of RAGE in the spinal dorsal horn and DRG of rats with type 2 diabetes mellitus.
