CAJ | 학술논문

目的评价青藤碱对神经病理性痛大鼠脊髓谷氨酸转运体-1(GLT-1)mRNA和谷氨酸-天冬氨酸转运体(GLAST) mRNA表达的影响.方法健康雄性Wistar大鼠72只,体重180～ 220 g,6～8周龄,采用随机数字表法分为3组(n=24):假手术组(S组)、神经病理性痛组(NP组)和青藤碱组(SIN组).NP组和SIN组采用坐骨神经慢性压迫损伤法制备神经病理性痛模型,S组仅分离坐骨神经但不结扎.SIN组于术毕即刻开始至处死前1d,腹腔注射盐酸青藤碱注射液40 mg/kg,1次/d,S组和NP组腹腔注射等容量生理盐水.于术前1 d(T0)、术后1、3、7和14 d(T1-4)时测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL);于T1-4时测定痛阈后处死6只大鼠,取L4-6脊髓组织,采用荧光定量PCR法检测脊髓背角GLT-1 mRNA和GLASTmRNA表达水平.结果与S组比较,NP组和SIN组T1-4时MWT降低,TWL缩短,NP组T1,2时脊髓背角GLT-1 mRNA和GLASTmRNA表达上调(P＜0.05),SIN组T1-3时GLT-1 mRNA及GLASTmRNA表达上调(P＜0.05);与NP组比较,SIN组MWT升高,TWL延长,脊髓背角GLT-1 mRNA和GLAST mRNA表达上调(P＜0.05).结论青藤碱减轻大鼠神经病理性痛的机制与上调脊髓GLT-1 mRNA和GLASTmRNA表达有关.
목적 평개청등감대신경병이성통대서척수곡안산전운체-1(GLT-1)mRNA화곡안산-천동안산전운체(GLAST) mRNA표체적영향.방법 건강웅성Wistar대서72지,체중180～ 220 g,6～8주령,채용수궤수자표법분위3조(n=24):가수술조(S조)、신경병이성통조(NP조)화청등감조(SIN조).NP조화SIN조채용좌골신경만성압박손상법제비신경병이성통모형,S조부분리좌골신경단불결찰.SIN조우술필즉각개시지처사전1d,복강주사염산청등감주사액40 mg/kg,1차/d,S조화NP조복강주사등용량생리염수.우술전1 d(T0)、술후1、3、7화14 d(T1-4)시측정궤계축족반응역(MWT)화열축족잠복기(TWL);우T1-4시측정통역후처사6지대서,취L4-6척수조직,채용형광정량PCR법검측척수배각GLT-1 mRNA화GLASTmRNA표체수평.결과 여S조비교,NP조화SIN조T1-4시MWT강저,TWL축단,NP조T1,2시척수배각GLT-1 mRNA화GLASTmRNA표체상조(P＜0.05),SIN조T1-3시GLT-1 mRNA급GLASTmRNA표체상조(P＜0.05);여NP조비교,SIN조MWT승고,TWL연장,척수배각GLT-1 mRNA화GLAST mRNA표체상조(P＜0.05).결론 청등감감경대서신경병이성통적궤제여상조척수GLT-1 mRNA화GLASTmRNA표체유관.
Objective To evaluate the effectsof sinomenine on the expression of glutamate transporter-1 (GLT-1) mRNA and glutamate aspartate transporter (GLAST) mRNA in the spinal cord of rats with neuropathic pain (NP).Methods Seventy-two male Wistar rats,weighing 180-220 g,aged 6-8 weeks,were divided into 3 groups (n =24 each) using a random number table:sham operation group (group S); NP group; sinomenine group (group SIN).The animals were anesthetized with intraperitoneal 10％ chloral hydrate 350 mg/kg.NP was induced by chronic constrictive injury.The right sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 silk thread.In group S,the right sciatic nerves were only exposed,but not ligated.In group SIN,sinomenine injection 40 mg/kg was injected intraperitoneally once a day starting from the end of operation until 1 day before the animals were sacrificed,while the equal volume of normal saline was given instead in S and NP groups.Mechanical paw withdrawal threshold to von Frey stimuli (MWT) and paw withdrawal latency to thermal nociceptive stimulus (TWL) were measured at 1 day before operation and 1,3,7 and 14 days after operation (T1-4).The rats were sacrificed after measurement of pain threshold,and their lumbar segments (L4-6) of the spinal cord were immediately removed for determination of GLT-1 mRNA and GLAST mRNA expression in the spinal dorsal horn (by real-time fluorescent quantitative PCR).Results Compared with group S,MWT was significantly decreased and TWL was shortened at T1-4 in NP and SIN groups,the expression of GLT-1 mRNA and GLAST mRNA was up-regulated at T1.2 in group NP,and the expression of GLT-1 mRNA and GLAST mRNA was up-regulated at T1-3 in group SIN.Compared with group NP,MWT was significantly increased,TWL was prolonged,and the expression of GLT-1 mRNA and GLAST mRNA was up-regulated at T1.3 in group SIN.Conclusion Sinomenine can mitigate NP in rats and up-regulation of GLT-1 mRNA and GLAST mRNA expression is involved in the mechanism.