CAJ | 학술논문

目的评价加巴喷丁治疗双侧坐骨神经结扎(bCCI)诱发大鼠神经病理性痛的效应.方法 SPF级健康雌性SD大鼠48只,体重180 ～ 200 g,7～9周龄,采用随机数字表法,将其分为4组(n=12):正常对照组(C组)、假手术组(S组)、bCCI组和bCCI+加巴喷丁组(G组).加巴喷丁给药方案:给药方式为腹腔注射,术前15 min给药1次,术后7-13 d期间2次/d,每次100 mg/kg,连续7d.分别于术前(基础水平)、术后1、3、6、8、10、14 d时记录双足机械缩足反应阈(MWT)、热缩足潜伏期(TWL)和冷刺激诱发缩足次数(TNCW).结果与C组比较,bCCI组和G组左足和右足MWT降低,TWL缩短(P＜0.05),S组上述指标差异无统计学意义(P＞0.05);与bCCI组比较,G组左足和右足MWT升高,TWL延长(P＜0.05),TNCW差异无统计学意义(P＞0.05).结论加巴喷丁可缓解bCCI诱发神经病理性痛大鼠机械痛觉过敏和热痛觉过敏,而对冷痛觉过敏无影响.
목적 평개가파분정치료쌍측좌골신경결찰(bCCI)유발대서신경병이성통적효응.방법 SPF급건강자성SD대서48지,체중180 ～ 200 g,7～9주령,채용수궤수자표법,장기분위4조(n=12):정상대조조(C조)、가수술조(S조)、bCCI조화bCCI+가파분정조(G조).가파분정급약방안:급약방식위복강주사,술전15 min급약1차,술후7-13 d기간2차/d,매차100 mg/kg,련속7d.분별우술전(기출수평)、술후1、3、6、8、10、14 d시기록쌍족궤계축족반응역(MWT)、열축족잠복기(TWL)화랭자격유발축족차수(TNCW).결과 여C조비교,bCCI조화G조좌족화우족MWT강저,TWL축단(P＜0.05),S조상술지표차이무통계학의의(P＞0.05);여bCCI조비교,G조좌족화우족MWT승고,TWL연장(P＜0.05),TNCW차이무통계학의의(P＞0.05).결론 가파분정가완해bCCI유발신경병이성통대서궤계통각과민화열통각과민,이대랭통각과민무영향.
Objective To evaluate the effect of gabapentin for treatment of neuropathic pain induced by bilateral sciatic nerve chronic constriction injury (bCCI) of rats.Methods Forty-eight female SPF Sprague-Dawley rats,weighing 180-200 g,aged 7-9 weeks,were randomly divided into 4 groups (n =12 each) using a random number table:control group (C group),sham operation group (S group),bCCI group,and bCCI + gabapentin group (G group).Gabapentin 100 mg/kg was intraperitoneally injected once at 15 min before operation and twice a day during 7-13 days after operation for 7 consecutive days.The bilateral mechanical paw withdrawal threshold (MWT),thermal paw withdrawal latency (TWL) and number of cold-stimulated paw withdrawal were measured before operation (baseline) and at 1,3,6,8,10,14 days after operation.Results Compared with C group,the bilateral MWT was significantly decreased,and the bilateral TWL was shortened in bCCI and G groups,and no significant change was found in MWT and TWL in S group.Compared with bCCI group,the bilateral MWT was significantly increased,the bilateral TWL was prolonged,and no significant change was found in the number of cold-stimulated paw withdrawal in G group.Conclusion Gabapentin can relieve thermal and mechanical hyperalgesia induced by bCCI,however,it exerts no effect on cold allodynia in rats.