CAJ | 학술논문

目的评价右美托咪定对烫伤大鼠细胞免疫功能的影响.方法健康雄性SD大鼠72只,体重200 ～ 220 g,日龄120～ 150 d.采用随机数字表法,将其分为3组(n=24):正常对照组(C组)、烫伤组(S组)、右美托咪定组(D组).大鼠背部区域脱毛后浸入94℃热水中12 s,制备30％体表面积烫伤模型.C组不做任何处理,S组制备烫伤模型后进行容量复苏治疗,D组制备烫伤模型后除进行容量复苏治疗外,同时于制备模型后即刻腹腔注射右美托咪定30μg/kg.于模型制备前(T1)、烫伤后12 h(T2)、24 h(T3)时采集下腔静脉血,测定T淋巴细胞亚群CD3+、CD4+、CD8+和NK细胞、C反应蛋白(CRP)、IL-6、IL-10及TNF-α的水平,计算CD4+/CD8+比值,并取股动脉血,进行血气分析.结果与C组比较,S组和D组T2.3时CD3+、CD4+、CD4+/CD8+比值、pH值、PaCO2、PaO2、NK细胞水平降低,CD8+、IL-6、IL-10、TNF-α及CRP水平升高,BE负值增加(P＜0.05).与S组比较,D组T23时CD3+、CD4+、CD4 +/CD8+比值、IL-10、pH值、PaCO2、PaO2、NK细胞水平升高,CD8+、IL-6、TNF-α及CRP水平降低,BE负值减少(P＜0.05).结论右美托咪定可改善烫伤大鼠细胞免疫功能.
목적 평개우미탁미정대탕상대서세포면역공능적영향.방법 건강웅성SD대서72지,체중200 ～ 220 g,일령120～ 150 d.채용수궤수자표법,장기분위3조(n=24):정상대조조(C조)、탕상조(S조)、우미탁미정조(D조).대서배부구역탈모후침입94℃열수중12 s,제비30％체표면적탕상모형.C조불주임하처리,S조제비탕상모형후진행용량복소치료,D조제비탕상모형후제진행용량복소치료외,동시우제비모형후즉각복강주사우미탁미정30μg/kg.우모형제비전(T1)、탕상후12 h(T2)、24 h(T3)시채집하강정맥혈,측정T림파세포아군CD3+、CD4+、CD8+화NK세포、C반응단백(CRP)、IL-6、IL-10급TNF-α적수평,계산CD4+/CD8+비치,병취고동맥혈,진행혈기분석.결과 여C조비교,S조화D조T2.3시CD3+、CD4+、CD4+/CD8+비치、pH치、PaCO2、PaO2、NK세포수평강저,CD8+、IL-6、IL-10、TNF-α급CRP수평승고,BE부치증가(P＜0.05).여S조비교,D조T23시CD3+、CD4+、CD4 +/CD8+비치、IL-10、pH치、PaCO2、PaO2、NK세포수평승고,CD8+、IL-6、TNF-α급CRP수평강저,BE부치감소(P＜0.05).결론 우미탁미정가개선탕상대서세포면역공능.
Objective To evaluate the effects of dexmedetomidine on the cellular immune function of the rats with scald.Methods Seventy-two healthy male Sprague-Dawley rats,weighing 200-220 g,aged 120-150 days,were randomly divided into 3 groups (n =24 each):normal control group (group C),scald group (group S) and dexmedetomidine group (group D).Thirty percent of the total body surface was shaved and then exposed to 94 ℃ water for 12 s in S and D groups.The rats were resuscitated according to Parkland formula after scald in S and D groups,and in addition,dexmedetomidine 30 μg/kg was also intraperitoneally injected immediately after scald in D group.Before the model was established (T1) and at 12 and 24 h after scald (T2,3),blood samples from the inferior vena cava were collected for determination of T lymphocyte subsets CD3 +,CD4 + and CD8 +,NK cell,C-reactive protein (CRP),interleukin-6 (IL-6),IL-10 and tumor necrosis factor-alpha (TNF-α) level.CD4+/CD8+ was calculated.Arterial blood samples were collected for blood gas analysis.Results Compared with C,the CD3+,CD4+ and NK cell levels,CD4+/CD8+,pH value,PaCO2 and PaO2 were significantly decreased,and CD8+ levels,IL-6,IL-10,TNF-α,CRP and BE negative value were increased at T2,3 in S and D groups.Compared with group S,the CD3+,CD4+,NK cell and IL-10 levels,CD4+/CD8+,pH value,PaCO2 and PaO2 were significantly increased,and CD8+ levels,IL-6,TNF-α,CRP and BE negative value were decreased at T2,3 in group D.Conclusion Dexmedetomidine can improve the cellular immune function of the rats with scald.