CAJ | 학술논문

目的了解肥胖儿童血浆各种超长链脂肪酸(VLCFAs)的变化,分析其与肥胖相关指标的关系.方法抽取7 ～12岁肥胖儿童166例为肥胖组,年龄、性别匹配的正常体重儿童148名为正常对照组,检测身高、体重及腰围,双能X线吸收法检测体脂百分比(PBF),抽取空腹血检测甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、空腹血糖和空腹胰岛素(FINS)水平,计算体重指数(BMI)、BMI-Z评分、腰围身高比(WHtR)和稳态模型评估的胰岛素抵抗指数(HOMA-IR).用气相色谱质谱联用仪检测血浆VLCFAs.结果 (1)与正常对照组比较,肥胖组的体重、BMI、BMI-Z评分、腰围、WHtR、PBF、TG、TC、LDL-C、FINS及HOMA-IR均升高,HDL-C水平降低(均P＜0.05).(2)肥胖组血浆总超长链单不饱和脂肪酸、超长链多不饱和脂肪酸、二十二碳烯酸(C22∶1n-9)及花生四烯酸(C20∶4n-6)较正常对照组降低,总超长链饱和脂肪酸、花生酸(C20∶0)和二十碳五烯酸(C20∶5n-3)升高.(3)直线相关分析显示PBF与C20∶0和C20∶5n-3呈正相关,与20∶4n-6呈负相关;WHtR与C22∶1n-9呈负相关,与C20∶0呈正相关.(4)多元逐步回归分析显示影响PBF的因素为C20∶0,影响WHtR的因素为C22∶1n-9和C20∶0.结论肥胖儿童存在超长链脂肪酸代谢谱紊乱,C20∶0、C22∶1n-9及C20∶5n-3的变化可能与肥胖相关.
목적 료해비반인동혈장각충초장련지방산(VLCFAs)적변화,분석기여비반상관지표적관계.방법 추취7 ～12세비반인동166례위비반조,년령、성별필배적정상체중인동148명위정상대조조,검측신고、체중급요위,쌍능X선흡수법검측체지백분비(PBF),추취공복혈검측감유삼지(TG)、총담고순(TC)、고밀도지단백담고순(HDL-C)、저밀도지단백담고순(LDL-C)、공복혈당화공복이도소(FINS)수평,계산체중지수(BMI)、BMI-Z평분、요위신고비(WHtR)화은태모형평고적이도소저항지수(HOMA-IR).용기상색보질보련용의검측혈장VLCFAs.결과 (1)여정상대조조비교,비반조적체중、BMI、BMI-Z평분、요위、WHtR、PBF、TG、TC、LDL-C、FINS급HOMA-IR균승고,HDL-C수평강저(균P＜0.05).(2)비반조혈장총초장련단불포화지방산、초장련다불포화지방산、이십이탄희산(C22∶1n-9)급화생사희산(C20∶4n-6)교정상대조조강저,총초장련포화지방산、화생산(C20∶0)화이십탄오희산(C20∶5n-3)승고.(3)직선상관분석현시PBF여C20∶0화C20∶5n-3정정상관,여20∶4n-6정부상관;WHtR여C22∶1n-9정부상관,여C20∶0정정상관.(4)다원축보회귀분석현시영향PBF적인소위C20∶0,영향WHtR적인소위C22∶1n-9화C20∶0.결론 비반인동존재초장련지방산대사보문란,C20∶0、C22∶1n-9급C20∶5n-3적변화가능여비반상관.
Objective To investigate the changes in plasma very long chain fatty acids (VLCFAs),and to explore its relationship with obesity-related index in obese children.Methods One hundred and sixty-six obese children aged 7 to 12 years old were investigated and 148 health children matched with age and sex were selected as control group.Their height,weight,and waist circumference (WC) were measured.The percentage of body fat (PBF) was tested by dual-energy X-ray absorptiometry.Fasting blood triglycerides (TG),total cholesterol (TC),high density lipoprotein-cholesterol (HDL-C),low density lipoprotein-cholesterol (LDL-C) levels,fasting plasma glucose,and fasting insulin (FINS) were determined.The body mass index (BMI),BMI-Z score,waist to height ratio (WHtR),and homeostasis model assessment for insulin resistance (HOMA-IR) were calculated.The plasma VLCFAs were analyzed by gas chromatography-mass spectrometry.Results (1) Compared with normal control children,body weight,BMI,BMI-Z score,WC,WHtR,PBF,TG,TC,LDL-C,FINS,and HOMA-IR in obese children were increased (all P ＜ 0.05),and the level of HDL-C was decreased (P ＜ 0.05).(2) The plasma levels of docosamonoenoic acid (C22 ∶ 1 n-9) and arachidonic acid (C20 ∶ 4n-6) were decreased while the levels of arachicacid (C20 ∶ 0) and eicosapentaenoic acid (C20 ∶ 5n-3) increased in obese children compared to non-obese children.(3) The linear correlation analysis showed that PBF was positively correlated with C20 ∶ 0 and C20 ∶ 5n-3,and negatively correlated with C20 ∶ 4n-6.WHtR was negatively correlated with C22 ∶ 1 n-9 and positively correlated with C20 ∶ 0 (4) The multiple stepwise regression analysis showed that C20 ∶ 0 was the main factor for PBF,and C22 ∶ 1 n-9 and C20 ∶ 0 were the main factors for WHtR.Conclusion The metabolic abnormality of plasma very long chain fatty acids exists in obese children.The changes in C20 ∶ 0,C22 ∶ 1 n-9,and C20 ∶ 5n-3 may be associated with obesity in children.