CAJ | 학술논문

以核因子κB受体活化因子配体(RANKL)和促黑素(α-MSH)孵育Raw264.7细胞6d,经抗酒石酸酸性磷酸酶染色观察破骨细胞形成的数目,并检测酸性磷酸酶活性.RT-PCR检测Raw264.7细胞5种黑皮质素受体(MCR)的表达.结果显示,与RANKL组相比较,RANKL+α-MSH组显著增加了破骨细胞的形成数量(P＜0.05),呈剂量依赖性,单独应用α-MSH组未见破骨细胞形成.RANKL组和RANKL+α-MSH组的酸性磷酸酶活性显著高于对照组和α-MSH组(P＜0.05),但前2组之间差异无统计学意义(P＞0.05);RT-PCR结果显示Raw264.7细胞5种MCR均表达.结果提示,α-MSH可能通过RANK信号通路促进破骨细胞形成.
이핵인자κB수체활화인자배체(RANKL)화촉흑소(α-MSH)부육Raw264.7세포6d,경항주석산산성린산매염색관찰파골세포형성적수목,병검측산성린산매활성.RT-PCR검측Raw264.7세포5충흑피질소수체(MCR)적표체.결과현시,여RANKL조상비교,RANKL+α-MSH조현저증가료파골세포적형성수량(P＜0.05),정제량의뢰성,단독응용α-MSH조미견파골세포형성.RANKL조화RANKL+α-MSH조적산성린산매활성현저고우대조조화α-MSH조(P＜0.05),단전2조지간차이무통계학의의(P＞0.05);RT-PCR결과현시Raw264.7세포5충MCR균표체.결과제시,α-MSH가능통과RANK신호통로촉진파골세포형성.
Raw264.7 cells were incubated with receptor activator of NF-kappa B ligand (RANKL) and α-melanocyte stimulating hormone(α-MSH) for6 d.The amount of osteoclast cells were counted by tartrate resistant acid phosphatase staining and the acid phosphatase activity was assayed.The expressions of 5 melanocortin receptors (MCR) in Raw264.7 cells were determined by RT-PCR.The results showed that the number of osteoclasts in RANKL +α-MSH group was significantly increased compared with RANKL group (P ＜ 0.05),but there was no osteoclast formation in α-MSH group.Compared with control group and α-MSH group,the acid phosphatase activities were significantly increased in RANKL group and α-MSH+RANKL group (P＜0.05).All five MCRs were expressed in the Raw264.7 cells shown by RT-PCR.These results suggest that α-MSH may promote osteoclasts formation through RANK signaling pathway.