CAJ | 학술논문

探讨血清8-羟基脱氧鸟嘌呤(8-hydroxy-deoxyguanosine,8-OHdG)水平在Graves眼病发病机制中的作用及与Graves眼病活动性的关系.用酶联免疫吸附法(ELISA)检测48例Graves眼病患者(GO组)和30例单纯Graves病而无眼征的患者(GD组)的血清8-OHdG水平,同时检测30名健康体检者作为对照组.与对照组相比,GO组和GD组的血清8-OHdG水平明显增高[(2.98±1.33),(2.07±1.30)对(0.72±0.93) ng/ml,P＜0.05]；且GO组的血清8-OHdG水平明显高于GD组(P＜0.05);Pearson相关性分析显示血清8-OHdG水平与临床疾病活动分数呈显著正相关(r=0.54,P＜0.01).Graves眼病患者外周血8-OHdG水平明显增高,提示氧化应激与Graves眼病的发病机制有关,血清8-OHdG水平可作为评价Graves眼病疾病活动度的良好指标.
탐토혈청8-간기탈양조표령(8-hydroxy-deoxyguanosine,8-OHdG)수평재Graves안병발병궤제중적작용급여Graves안병활동성적관계.용매련면역흡부법(ELISA)검측48례Graves안병환자(GO조)화30례단순Graves병이무안정적환자(GD조)적혈청8-OHdG수평,동시검측30명건강체검자작위대조조.여대조조상비,GO조화GD조적혈청8-OHdG수평명현증고[(2.98±1.33),(2.07±1.30)대(0.72±0.93) ng/ml,P＜0.05]；차GO조적혈청8-OHdG수평명현고우GD조(P＜0.05);Pearson상관성분석현시혈청8-OHdG수평여림상질병활동분수정현저정상관(r=0.54,P＜0.01).Graves안병환자외주혈8-OHdG수평명현증고,제시양화응격여Graves안병적발병궤제유관,혈청8-OHdG수평가작위평개Graves안병질병활동도적량호지표.
To investigate the role played by 8-hydroxy-deoxyguanosine (8-OHdG) in patients with Graves'ophthalmopathy(GO),and the association between 8-OHdG and the disease activity of GO.Serum levels of 8-OHdG were tested in 48 cases with GO and 30 cases with Graves' disease(GD) without ophthalmopathy.30 healthy subjects participated in this study as controls.8-OHdG was detected by enzyme-linked immunosorbent assay (ELISA).Compared with the controls,the mean level of 8-OHdG in GO patients and GD patients was increased significantly [(2.98±1.33),(2.07±1.30) vs(0.72±0.93)ng/ml,P＜0.05].Mean 8-OHdG level in GO patients was markedly higher when compared to GD patients(P＜0.05).Pearson regression analysis showed that elevated levels of 8-OHdG were significantly correlated with the Clinical Activity Score of GO (r =0.54,P＜0.01).The serum level of 8-OHdG in GO patients was raised obviously,it implies that oxidative stress plays an important role in the pathogenesis of GO,and the serum level of 8-OHdG might act as an activity index of GO.