CAJ | 학술논문

目的观察胰升糖素样肽1(GLP-1)类似物利拉鲁肽对正常人和新发1型糖尿病患者外周血CD4+CD25-T细胞增殖的影响,评价利拉鲁肽对1型糖尿病可能的免疫调节作用.方法免疫磁珠法分离10名正常人和10例新发1型糖尿病患者的外周血CD4+ CD25 T细胞,用CD3/CD28单抗包被的T细胞扩增磁珠刺激增殖.以CFSE染色通过流式细胞术比较在不同浓度利拉鲁肽(0、25、50和100 nmol/ml)干预后,对CD4+C D25-T细胞增殖的影响.结果 (1)利拉鲁肽剂量依赖性抑制正常人和新发1型糖尿病患者的CD4+ CD25-T细胞的增殖(各干预浓度组间P＜0.05)；(2)在利拉鲁肽各干预浓度组,1型糖尿病患者的CD4+CD25-T细胞的增殖作用显著强于正常人的CD4+ CD25-T细胞(P＜0.01)；(3)利拉鲁肽对正常人和新发1型糖尿病患者的CD4+CD25 T细胞增殖的抑制程度则无明显差别(P＞0.05).结论 1型糖尿病患者的CD4+ CD25-T细胞的增殖作用显著增强,提示1型糖尿病患者存在细胞免疫紊乱.利拉鲁肽在体外对CD4+ CD25-T细胞的增殖具有抑制作用,这种免疫负性调节作用使其在1型糖尿病治疗方面具有潜在的应用价值.
목적 관찰이승당소양태1(GLP-1)유사물리랍로태대정상인화신발1형당뇨병환자외주혈CD4+CD25-T세포증식적영향,평개리랍로태대1형당뇨병가능적면역조절작용.방법 면역자주법분리10명정상인화10례신발1형당뇨병환자적외주혈CD4+ CD25 T세포,용CD3/CD28단항포피적T세포확증자주자격증식.이CFSE염색통과류식세포술비교재불동농도리랍로태(0、25、50화100 nmol/ml)간예후,대CD4+C D25-T세포증식적영향.결과 (1)리랍로태제량의뢰성억제정상인화신발1형당뇨병환자적CD4+ CD25-T세포적증식(각간예농도조간P＜0.05)；(2)재리랍로태각간예농도조,1형당뇨병환자적CD4+CD25-T세포적증식작용현저강우정상인적CD4+ CD25-T세포(P＜0.01)；(3)리랍로태대정상인화신발1형당뇨병환자적CD4+CD25 T세포증식적억제정도칙무명현차별(P＞0.05).결론 1형당뇨병환자적CD4+ CD25-T세포적증식작용현저증강,제시1형당뇨병환자존재세포면역문란.리랍로태재체외대CD4+ CD25-T세포적증식구유억제작용,저충면역부성조절작용사기재1형당뇨병치료방면구유잠재적응용개치.
Objective To study the role of glucagon-like peptide-1 (GLP-1) analogue liraglutide played in the proliferation of CD4+CD25 T cells in normal people and newly-onset type 1 diabetic patients,and to evaluate the possible immune regulatory role of liraglutide in the therapy of type 1 diabetes.Methods CD4+ CD25-T cells of 10 normal people and 10 newly-onset type 1 diabetic patients were separated from peripheral blood by MACS immunomagnetic beads and stimulated by Human T-Activator CD3/CD28 Dynabeads to proliferate.CFSE labeling technique was used to evaluate the proliferation of CD4+ CD25-T cells by flow cytometry.Liraglutide of different concentrations(0,25,50,and 100 nmol/ml) was added to the proliferation system,then the proliferation of CD4+CD25-T cell was measured.Results (1) Liraglutide suppressed the proliferation of CD4+ CD25-T cells from either normal people or type 1 diahetic patients with dose-dependent manner (P ＜ 0.05).(2) Under the different concentrationsofliraglutide,the proliferation ofCD4+CD25 T cells from diabetic patients was mueh more robust than that of normal people (P＜0.01).(3) The inhibitory effects of liraglutide on CD4+ CD25-T cells proliferation in normal people and diabetic patients were similar (P＞0.05).Conclusion The proliferation of CD4+ CD25 T cells in type 1 diabetic patients was more robust than normal people,which indicated cellular immune dysfunction in type 1diabetes.Liraglutide inhibits the proliferation of CD4+ CD25-T cells of type 1 diabetic patients in vitro.The immunosuppression effect of liraglutide may have potential value in the treatment of type 1 diabetes.