CAJ | 학술논문

目的检测2型糖尿病患者及肥胖患者血浆颗粒蛋白前体(PGRN)水平,探讨其与慢性炎症及胰岛素抵抗的关系.方法 80例2型糖尿病(T2DM)患者和88名正常糖耐量(NGT)个体以体重指数(BMI) ≥25 kg/m2为切点分别分为正常体重(NW)亚组和肥胖(OB)亚组,检测空腹血糖(FPG)、糖负荷后2h血糖(2hPG)、HbA1C、血脂、空腹胰岛素(FINS)水平,计算BMI和腰臀比,并以稳态模型评估胰岛素抵抗指数(HOMA-IR)及胰岛β细胞功能(HOMA-β).采用ELISA法检测血浆PGRN及白细胞介素6(IL-6)水平.结果血浆PGRN水平在T2DM组高于NGT组(P＜0.01),在T2DM组和NGT组内,OB亚组PGRN水平高于对应NW亚组[(225.22±34.39对195.59±50.47和183.79 ±61.63对148.69±55.27)ng/ml,P＜0.05],相关性分析提示血浆PGRN水平与体重、腰围、BMI、收缩压、FPG、2hPG、HbA1C、甘油三酯(TG)、IL-6、FINS、HOMA-IR呈正相关(P＜0.05或P＜0.01),与HOMA-β呈负相关(P＜0.05).多元线性回归分析提示BMI、HbA1C、IL-6及TG是血浆PGRN水平的独立影响因素(P＜0.05).结论 2型糖尿病及肥胖患者血浆PGRN水平均升高,其升高程度与糖脂代谢紊乱、炎症及胰岛素抵抗的程度密切相关.
목적 검측2형당뇨병환자급비반환자혈장과립단백전체(PGRN)수평,탐토기여만성염증급이도소저항적관계.방법 80례2형당뇨병(T2DM)환자화88명정상당내량(NGT)개체이체중지수(BMI) ≥25 kg/m2위절점분별분위정상체중(NW)아조화비반(OB)아조,검측공복혈당(FPG)、당부하후2h혈당(2hPG)、HbA1C、혈지、공복이도소(FINS)수평,계산BMI화요둔비,병이은태모형평고이도소저항지수(HOMA-IR)급이도β세포공능(HOMA-β).채용ELISA법검측혈장PGRN급백세포개소6(IL-6)수평.결과 혈장PGRN수평재T2DM조고우NGT조(P＜0.01),재T2DM조화NGT조내,OB아조PGRN수평고우대응NW아조[(225.22±34.39대195.59±50.47화183.79 ±61.63대148.69±55.27)ng/ml,P＜0.05],상관성분석제시혈장PGRN수평여체중、요위、BMI、수축압、FPG、2hPG、HbA1C、감유삼지(TG)、IL-6、FINS、HOMA-IR정정상관(P＜0.05혹P＜0.01),여HOMA-β정부상관(P＜0.05).다원선성회귀분석제시BMI、HbA1C、IL-6급TG시혈장PGRN수평적독립영향인소(P＜0.05).결론 2형당뇨병급비반환자혈장PGRN수평균승고,기승고정도여당지대사문란、염증급이도소저항적정도밀절상관.
Objective To detect plasma progranulin (PGRN) level in subjects with newly diagnosed type 2 diabetes mellitus and to investigate the relationship of plasma PGRN level with glycolipid metabolism,inflammation,and insulin resistance.Methods Eighty patients with newly diagnosed type 2 diabetes (T2DM) and 88 subjects with normal glucose tolerance (NGT) were recruited in the study.Both of them were divided into normal weight (NW)subgroup and obesity (OB) subgroup.Obesity was defined as body mass index (BMI) ≥ 25 kg/m2 according to the World Health Organization-Western Pacific Region diagnostic criteria(2000).Body fat parameters were measured and BMI,waist-to-hip ratio were determined.Fasting plasma PGRN and interleukin-6 (IL-6) levels were determined by ELISA,fasting plasma glucose (FPG),2 h plasma glucose after glucose loading (2hPG),HbA1C,fasting insulin (FINS),and lipids were also detected.Insulin resistance and pancreas β cell function were assessed by homeostasis model assessment (HOMA-IR,HOMA-β).Results Plasma PGRN level was significantly higher in T2DM group than that in NGT group(P＜0.01).Within groups of T2DM and NGT,plasma PGRN level in OB subgroups was higher than that in NW subgroups [(225.22 ± 34.39 vs 195.59 ± 50.47 and 183.79 ± 61.63 vs 148.69 ± 55.27) ng/ml,P＜0.05].Bivariate correlation analysis showed that plasma PGRN level was positively correlated with weight,waist circumference,BMI,systolic blood pressure,FPG,2hPG,HbA1C,triglyceride(TG),IL-6,FINS,and HOMA-IR (P＜0.05 or P＜0.01),and was negatively correlated with HOMA-β (P＜0.05).Multiple linear regression analysis showed that BMI,HbA1C,IL-6,and TG were independently related to plasma PGRN level(P＜0.05).Conclusions Plasma PGRN level was increased in patients with type 2 diabetes as well as in obesity,and was closely related with glycolipid metabolism,inflammation,and insulin resistance.