CAJ | 학술논문

目的探讨骨桥蛋白在Graves病(GD)中的表达情况及骨桥蛋白对CD40配体的调节作用.方法选取初发Graves病患者30例,Graves病缓解患者21例,正常志愿者20名,采用酶联免疫吸附试验(ELISA)检测患者与志愿者血浆中骨桥蛋白和可溶型CD40配体水平,运用实时荧光定量PCR检测外周血单个核细胞骨桥蛋白受体mRNA的表达和CD4+T细胞中CD40配体mRNA的表达.结果 Graves病初发患者血浆骨桥蛋白水平显著高于对照组[(153±55对52±21)ng/ml,P＜0.01],而Graves病缓解患者血浆骨桥蛋白水平与对照组相比无统计学差异[(64± 19) ng/ml,P＞0.05]；与对照组比较,Graves病初发患者血浆可溶型CD40配体水平显著升高[(3031±1074对1073±351)pg/ml,P＜0.01],Graves病缓解患者可溶型CD40配体水平与对照组比较,差异无统计学意义[(974±273) pg/ml,P＞0.05]；血浆骨桥蛋白与可溶型CD40配体水平呈显著正相关(r=0.849,P＜0.01)；人重组骨桥蛋白可促进可溶型CD40配体和CD4+T细胞中CD40配体基因mRNA的表达.结论骨桥蛋白在Graves病初发患者血浆中表达明显增加,可通过促进CD40配体的表达参与Graves病发生、发展的免疫调节过程.
목적 탐토골교단백재Graves병(GD)중적표체정황급골교단백대CD40배체적조절작용.방법선취초발Graves병환자30례,Graves병완해환자21례,정상지원자20명,채용매련면역흡부시험(ELISA)검측환자여지원자혈장중골교단백화가용형CD40배체수평,운용실시형광정량PCR검측외주혈단개핵세포골교단백수체mRNA적표체화CD4+T세포중CD40배체mRNA적표체.결과 Graves병초발환자혈장골교단백수평현저고우대조조[(153±55대52±21)ng/ml,P＜0.01],이Graves병완해환자혈장골교단백수평여대조조상비무통계학차이[(64± 19) ng/ml,P＞0.05]；여대조조비교,Graves병초발환자혈장가용형CD40배체수평현저승고[(3031±1074대1073±351)pg/ml,P＜0.01],Graves병완해환자가용형CD40배체수평여대조조비교,차이무통계학의의[(974±273) pg/ml,P＞0.05]；혈장골교단백여가용형CD40배체수평정현저정상관(r=0.849,P＜0.01)；인중조골교단백가촉진가용형CD40배체화CD4+T세포중CD40배체기인mRNA적표체.결론골교단백재Graves병초발환자혈장중표체명현증가,가통과촉진CD40배체적표체삼여Graves병발생、발전적면역조절과정.
Objective To investigate the expression of osteopontin(OPN) in Graves' disease(GD),and to study the role played by OPN in modulating CD40 ligand expression.Methods Thirty initial GD patients,twenty-one GD patients during remission,and twenty healthy controls were recruited.Concentrations of OPN and soluble CD40 ligand were determined by ELISA.The expressions of OPN receptor genes in peripheral blood mononuclear cells and CD40 ligand gene in CD4+T cell were determined by real-time quantitative PCR.Results Plasma OPN level in GD patients was significantly higher than that in normal controls[(153 ±55 vs 52±21) ng/ml,P＜0.01],while the level in GD patients during remission[(64 ± 19 vs 52 ± 21) ng/ml,P＞0.05] showed no significant changes.Compared to healthy controls,soluble CD40 ligand was enhanced in GD patients [(3031 ± 1074 vs 1073 ± 351) pg/ml,P＜0.01]and in patients during remission,no significant differences found [(974 ± 273 vs 1073 ± 351) pg/ml,P＞0.05].Plasma OPN was correlated with soluble CD40 ligand concentration(r=0.849,P＜0.01),while OPN could increase soluble CD40 ligand level and CD40 ligand mRNA expression in CD4+T cells.Conclusions CD40 ligand is induced by increased OPN and serves as the downstream effector of OPN in GD development.