中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2013年
8期
696-699
,共4页
阳琰%高琳%邓华聪%晏永慧%李显文%王小英%廖鑫%张晗
暘琰%高琳%鄧華聰%晏永慧%李顯文%王小英%廖鑫%張晗
양염%고림%산화총%안영혜%리현문%왕소영%료흠%장함
脂联素%糖尿病,实验性%PPARα%胆固醇调节元件结合蛋白1C%载脂蛋白A5%甘油三酯
脂聯素%糖尿病,實驗性%PPARα%膽固醇調節元件結閤蛋白1C%載脂蛋白A5%甘油三酯
지련소%당뇨병,실험성%PPARα%담고순조절원건결합단백1C%재지단백A5%감유삼지
Adiponectin%Diabetes mellitus,experimental%PPARα%Sterol regulatory element binding proteins-1C%Apolipoprotein A5%Triglyceride
目的 观察脂联素对糖尿病大鼠模型肝脏中PPARα、胆固醇调节元件结合蛋白1C(SREBP-1C)、载脂蛋白A5(apoA5)表达及甘油三酯含量的影响.方法 取60只雄性Wistar大鼠分成单纯糖尿病组(DM,n=20)、脂联素治疗组(Ad-APN+DM,n=20)和对照组(CON,n=20).每组大鼠各取10只,采用实时荧光定量逆转录-聚合酶链反应(RT-PCR)、Western印迹分别对大鼠的肝脏组织中PPARα、SREBP-1C及apoA5基因、蛋白表达水平进行检测,并检测肝脏组织中甘油三酯含量.结果 (1)DM组肝脏中PPARα、apoA5基因及蛋白表达比对照组显著降低(P＜0.05),SREBP-1C基因、蛋白表达及甘油三酯含量比对照组显著增加(P＜0.05)；(2)脂联素干预组肝脏中PPARα、apoA5基因及蛋白表达较DM组增加(P＜0.05),而SREBP-1C表达下降(P＜0.05),甘油三酯含量明显降低(P＜0.05)；(3)脂联素干预组肝脏中PPARα、SREBP-1C、apoA5基因及蛋白表达较对照组差异无统计学意义(P＞0.05),甘油三酯含量比较也无显著性差异(P＞0.05).结论 脂联素可能通过上调肝脏中PPARα、apoA5表达,抑制SREBP-1C的表达,从而降低甘油三酯水平.
目的 觀察脂聯素對糖尿病大鼠模型肝髒中PPARα、膽固醇調節元件結閤蛋白1C(SREBP-1C)、載脂蛋白A5(apoA5)錶達及甘油三酯含量的影響.方法 取60隻雄性Wistar大鼠分成單純糖尿病組(DM,n=20)、脂聯素治療組(Ad-APN+DM,n=20)和對照組(CON,n=20).每組大鼠各取10隻,採用實時熒光定量逆轉錄-聚閤酶鏈反應(RT-PCR)、Western印跡分彆對大鼠的肝髒組織中PPARα、SREBP-1C及apoA5基因、蛋白錶達水平進行檢測,併檢測肝髒組織中甘油三酯含量.結果 (1)DM組肝髒中PPARα、apoA5基因及蛋白錶達比對照組顯著降低(P＜0.05),SREBP-1C基因、蛋白錶達及甘油三酯含量比對照組顯著增加(P＜0.05)；(2)脂聯素榦預組肝髒中PPARα、apoA5基因及蛋白錶達較DM組增加(P＜0.05),而SREBP-1C錶達下降(P＜0.05),甘油三酯含量明顯降低(P＜0.05)；(3)脂聯素榦預組肝髒中PPARα、SREBP-1C、apoA5基因及蛋白錶達較對照組差異無統計學意義(P＞0.05),甘油三酯含量比較也無顯著性差異(P＞0.05).結論 脂聯素可能通過上調肝髒中PPARα、apoA5錶達,抑製SREBP-1C的錶達,從而降低甘油三酯水平.
목적 관찰지련소대당뇨병대서모형간장중PPARα、담고순조절원건결합단백1C(SREBP-1C)、재지단백A5(apoA5)표체급감유삼지함량적영향.방법 취60지웅성Wistar대서분성단순당뇨병조(DM,n=20)、지련소치료조(Ad-APN+DM,n=20)화대조조(CON,n=20).매조대서각취10지,채용실시형광정량역전록-취합매련반응(RT-PCR)、Western인적분별대대서적간장조직중PPARα、SREBP-1C급apoA5기인、단백표체수평진행검측,병검측간장조직중감유삼지함량.결과 (1)DM조간장중PPARα、apoA5기인급단백표체비대조조현저강저(P＜0.05),SREBP-1C기인、단백표체급감유삼지함량비대조조현저증가(P＜0.05)；(2)지련소간예조간장중PPARα、apoA5기인급단백표체교DM조증가(P＜0.05),이SREBP-1C표체하강(P＜0.05),감유삼지함량명현강저(P＜0.05)；(3)지련소간예조간장중PPARα、SREBP-1C、apoA5기인급단백표체교대조조차이무통계학의의(P＞0.05),감유삼지함량비교야무현저성차이(P＞0.05).결론 지련소가능통과상조간장중PPARα、apoA5표체,억제SREBP-1C적표체,종이강저감유삼지수평.
Objective To observe the gene and protein expression of PPARα,sterol regulatory element binding proteins-1C (SREBP-1C),and apolipoprotein (apo) A5 under adiponectin intervention,as well as triglyceride level in liver in diabetic rats.Methods Sixty male Wistar rats were assigned into 3 groups in random:diabetic group(DM,n =20),adiponectin treated group (Ad-APN + DM,n =20),and control group (CON,n =20).10 rats from each group were taken to detect the gene and protein expression of PPARα,SREBP-1C,and apoA5 in liver by real time-fluorescent quantitation reverse transcription-polymerase chain reaction and Western blot.The level of triglyceride in liver was determined.Results (1) The gene and protein expressions of PPARα,apoA5 were decreased in DM group(vs CON,P＜0.05).The expression of SREBP-1C and the level of triglyceride were increased (P＜0.05).(2) The expression of PPARα and apoA5 in Ad-APN+DM group were increased while the expression of SREBP-1C was decreased(all P＜0.05).(3) No difference was found in the expressions of PPARα,SREBP-1C,and apoA5,as well as the levels of triglyceride between Ad-APN + DM group and CON group (P＞0.05).Conclusions Adiponectin may upregulate PPARα and apoA5 expressions but inhibit SREBP-1C expression in liver of diabetic rats,leading to decreased triglyceride content in liver of rats.
