CAJ | 학술논문

目的探讨锌指蛋白217 (ZNF217)在人类甲状腺乳头状癌组织中的表达及其意义.方法利用实时荧光定量PCR法和Western印迹法检测20例甲状腺乳头状癌组织及相应的20例癌旁正常组织中ZNF217的mRNA和蛋白表达水平,并进行统计学分析.结果 ZNF217 mRNA和蛋白在甲状腺乳头状癌组织中的表达显著高于癌旁正常甲状腺组织(96.72±44.19对4.86±3.55,0.994±0.172对0.195±0.061,均P＜0.01),在甲状腺乳头状癌包膜侵犯组高于无包膜侵犯组(P＜0.01).ZNF217 mRNA和蛋白的表达与患者的性别、年龄、肿瘤大小、TNM分期及有无淋巴结转移均无关(均P＞0.05).结论 ZNF217的过表达可能与甲状腺乳头状癌的发生发展和包膜侵犯有关,有望成为甲状腺乳头状癌预防和治疗的一个新的靶点.
목적 탐토자지단백217 (ZNF217)재인류갑상선유두상암조직중적표체급기의의.방법 이용실시형광정량PCR법화Western인적법검측20례갑상선유두상암조직급상응적20례암방정상조직중ZNF217적mRNA화단백표체수평,병진행통계학분석.결과 ZNF217 mRNA화단백재갑상선유두상암조직중적표체현저고우암방정상갑상선조직(96.72±44.19대4.86±3.55,0.994±0.172대0.195±0.061,균P＜0.01),재갑상선유두상암포막침범조고우무포막침범조(P＜0.01).ZNF217 mRNA화단백적표체여환자적성별、년령、종류대소、TNM분기급유무림파결전이균무관(균P＞0.05).결론 ZNF217적과표체가능여갑상선유두상암적발생발전화포막침범유관,유망성위갑상선유두상암예방화치료적일개신적파점.
Objective To investigate the expression and clinical significance of zinc finger gene 217 (ZNF217) in human papillary thyroid carcinoma.Methods The expressions of ZNF217 mRNA and protein were detected by quantitative realtime PCR and Western blot in papillary thyroid carcinoma tissues (n =20) and adjacent normal tissues (n =20),and the data were analyzed.Results The expressions of ZNF217 mRNA and protein in papillary thyroid carcinoma were significantly higher than those in adjacent normal tissues (96.72 ± 44.19 vs 4.86 ±3.55,0.994 ± 0.172 vs 0.195 ± 0.061,both P＜0.01),being higher in the papillary thyroid carcinoma with capsule invasion compared with that without capsule invasion (P＜0.01).The expressions of ZNF217 mRNA and protein in papillary thyroid carcinoma were not related to gender,age,tumor size,TNM stage or lymph node metastasis (all P＞0.05).Conclusions The overexpression of ZNF217 may be associated with the oncogenesis and progress of papillary thyroid carcinoma and capsule invasion,and thus is expected to become a new target for prevention and treatment of papillary thyroid carcinoma.