CAJ | 학술논문

采用双向凝胶电泳和基质辅助激光解吸飞行时间质谱(MALDI-TOF-MS)对维吾尔族高尿酸血症人群和对照组人群血清进行差异蛋白质研究,并用Western印迹法进行差异蛋白质验证.结果显示,差异表达蛋白质点数为11个,质谱成功鉴定出4个差异蛋白质,分别是补体C3、触珠蛋白、补体C4和载脂蛋白A1,均呈上调表达.Western印迹法验证了补体C3在2组人群中的差异与蛋白质组学结果相一致.
채용쌍향응효전영화기질보조격광해흡비행시간질보(MALDI-TOF-MS)대유오이족고뇨산혈증인군화대조조인군혈청진행차이단백질연구,병용Western인적법진행차이단백질험증.결과현시,차이표체단백질점수위11개,질보성공감정출4개차이단백질,분별시보체C3、촉주단백、보체C4화재지단백A1,균정상조표체.Western인적법험증료보체C3재2조인군중적차이여단백질조학결과상일치.
2-Dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS) were used to screen differentially expressed serum proteins in Uyghur patients with hyperuricemia and control subjects.Western blot was used to validate differential proteins.Gel-image analysis revealed that there were 11 differential protein spots.A total of 4 differential protein spots were successfully identified by MALDI-TOF-MS.Complement 3,complement 4,haptoglobin,and apolipoprotein Al levels were up-regulated in patients as compared with controls.The result of complement 3 expression was validated by Western blot,which is accordant with that of proteomics.