CAJ | 학술논문

32只雄性SD大鼠分为正常对照组(n=16)和果糖组(n=16),分别予以正常饮水和10％果糖4周,2组再分别以生理盐水和50 nmol/kg胃促生长素(Ghrelin)干预6周(每组n=8),测定空腹血糖、血脂和胰岛素等生化指标,RT-PCR检测大鼠骨骼肌胰岛素受体(Ins-R) mRNA表达水平,Western印迹法检测胰岛素受体底物1(IRS-1)的磷酸化水平.结果显示,高果糖组大鼠血浆胰岛素浓度和稳态模型评估的胰岛素抵抗指数(HOMA-IR)均较正常对照组明显升高[(13.1±3.6对9.0±1.5)μU/ml,P＜O.05；2.78±0.14对1.81 ±0.13,P＜0.01)],而高果糖喂养的大鼠经Ghrelin干预后胰岛素水平[(9.6±2.5) μU/ml,P＜0.05]和HOMA-IR(1.96±0.12,P＜0.01)明显降低,骨骼肌Ins-R mRNA表达和IRS-1磷酸化水平明显升高(P＜0.01),提示Ghrelin可能通过恢复骨骼肌Ins-R和受体后功能,改善胰岛素抵抗.
32지웅성SD대서분위정상대조조(n=16)화과당조(n=16),분별여이정상음수화10％과당4주,2조재분별이생리염수화50 nmol/kg위촉생장소(Ghrelin)간예6주(매조n=8),측정공복혈당、혈지화이도소등생화지표,RT-PCR검측대서골격기이도소수체(Ins-R) mRNA표체수평,Western인적법검측이도소수체저물1(IRS-1)적린산화수평.결과현시,고과당조대서혈장이도소농도화은태모형평고적이도소저항지수(HOMA-IR)균교정상대조조명현승고[(13.1±3.6대9.0±1.5)μU/ml,P＜O.05；2.78±0.14대1.81 ±0.13,P＜0.01)],이고과당위양적대서경Ghrelin간예후이도소수평[(9.6±2.5) μU/ml,P＜0.05]화HOMA-IR(1.96±0.12,P＜0.01)명현강저,골격기Ins-R mRNA표체화IRS-1린산화수평명현승고(P＜0.01),제시Ghrelin가능통과회복골격기Ins-R화수체후공능,개선이도소저항.
Thirty-two male Sprague-Dawley rats were divided into control group (n =16) and fructose group (n =16) fed with 10％ fructose solution.After 4 weeks,the rats of two groups were treated with saline and 50 nmol/kg ghrelin for 6 weeks,respectively (each group n =8).Fasting plasma glucose,insulin,and blood lipid profile were measured.Insulin receptor (Ins-R) mRNA expression in muscle was detected by RT-PCR.The phosphorylation of insulin receptor substrate-1 (IRS-1) was measured by Western blot.The results showed that insulin level and homeostasis model assessment for insulin resistance index (HOMA-IR) in fructose group were higher than those in control group [(13.1±3.6 vs 9.0 ± 1.5) μU/ml,P＜0.05 ;2.78 ± 0.14 vs 1.81± 0.13,P ＜0.01)].After ghrelin treatment,plasma insulin concentration [(9.6 ± 2.5) μU/mL,P＜0.05] and HOMA-IR (1.96 ± 0.12,P＜0.01)significantly decreased,along with increased Ins-R mRNA and IRS-1 phosphorylation in skeletal muscle (P ＜0.01).These results suggest that ghrelin can ameliorate insulin resistance in fructose-fed rats by restoring Ins-R function.