CAJ | 학술논문

目的检测胰升糖素样肽1(GLP-1)是否调节内脂素的合成,并探讨其作用机制.方法以3T3-L1分化成熟脂肪细胞为模型,GLP-1干预后,提取总RNA定量检测内脂素转录水平,收集培养液酶联免疫法检测内脂素分泌水平;PKA通路抑制剂H89预处理30 min,检测内脂素的转录水平.结果 GLP-1呈剂量依赖性增加3T3-L1细胞表达内脂素,10-10mol/L时显著提高(P＜0.05),10-9 mol/L时达峰值(P＜0.0l);并呈时间依赖性,作用18h时内脂素显著升高(P＜0.05).H89部分阻断GLP-1刺激的内脂素表达.结论 GLP-1通过PKA途径提升脂肪细胞表达内脂素,进而可能提升胰岛功能和周围组织胰岛素敏感性.
목적 검측이승당소양태1(GLP-1)시부조절내지소적합성,병탐토기작용궤제.방법 이3T3-L1분화성숙지방세포위모형,GLP-1간예후,제취총RNA정량검측내지소전록수평,수집배양액매련면역법검측내지소분비수평;PKA통로억제제H89예처리30 min,검측내지소적전록수평.결과 GLP-1정제량의뢰성증가3T3-L1세포표체내지소,10-10mol/L시현저제고(P＜0.05),10-9 mol/L시체봉치(P＜0.0l);병정시간의뢰성,작용18h시내지소현저승고(P＜0.05).H89부분조단GLP-1자격적내지소표체.결론 GLP-1통과PKA도경제승지방세포표체내지소,진이가능제승이도공능화주위조직이도소민감성.
Objective To test whether glucagon like peptide-1 (GLP-1) would regulate the expression of visfatin in adipocytes,and to explore the mechanism of this effect.Methods Fully differentiated 3T3-L1 adipocytes were treated with GLP-1.Total RNA was extracted for analyzing the level of visfatin mRNA by quantitative RT-PCR.The media were collected for measuring the level of visfatin protein by enzyme linked immuno-assay (ELISA).In order to test the involvement of PKA pathway,the adipocytes were pretreated with a specific pharmacological PKA inhibitor H89 for 30 min before GLP-1 was added.Results GLP-1 increased visfatin expression in a time-and dose-dependent manner.The level of visfatin significantly increased at the concentration of 10 10 mol/L GLP-1 (P＜0.05),and reached the peak at 10-9 mol/L (P＜0.01).After incubation for 18 hours,GLP-1 dominantly increased the level of visfatin (P＜0.05).Inhibition of PKA pathway by H89 partially blocked the effect of GLP-1 on visfatin expression.Conclusions GLP-1 may enhance the expression of visfatin in 3T3-L1 adipocyte via the PKA pathway,which might contribute to the improvement in glucose homeostasis.