CAJ | 학술논문

目的观察白藜芦醇对急性痛风性关节炎大鼠血清及关节液中趋化因子CCR5、CXCL10的影响.方法选取Wista大鼠36只,按随机数字表法分为空白对照组、模型组、秋水仙碱组、白藜芦醇低剂量组、白藜芦醇中剂量组、白藜芦醇高剂量组,各组相应采用生理盐水、秋水仙碱、白藜芦醇低、中、高剂量灌胃7d(1次/d),模型组及各实验组于灌胃第4天将25 mg/ml(0.05 ml)浓度的尿酸盐溶液注射到大鼠踝关节腔内,制备急性痛风性关节炎模型,空白对照组大鼠关节腔内注射生理盐水0.05 ml,分别于造模后2、9、24、48、72 h取血,72 h后留取踝关节关节液,观察各组不同时间点关节肿胀指数的变化及血清关节液中白细胞介素(IL)-1β、CCR5、CXCL10的变化.结果关节液中,低、中、高剂量组IL-1β含量分别为(9.63±0.71)、(7.67±0.48)和(6.66 ±0.29) pg/ml,与模型组(11.85±0.45) pg/ml比较明显下降(P＜0.05),中、高剂量组CCR5含量分别为(4.49±0.25)和(4.65±0.25) ng/ml,与模型组(5.64±0.28) ng/ml比较明显下降(P＜0.05),中高剂量组CXCL10含量分别为(86.79±2.90)和(89.61±5.47) pg/ml,与模型组(110.13±4.02) pg/ml比较明显下降(P＜0.05),病理结果显示,白藜芦醇可减轻急性痛风性关节炎大鼠踝关节组织的水肿和炎性细胞浸润.结论急性痛风性关节炎发病过程中IL-1β、趋化因子CCR5、CXCL10明显增高,白藜芦醇可有效抑制急性痛风性关节炎发作,且该作用呈一定的剂量依赖性.
목적 관찰백려호순대급성통풍성관절염대서혈청급관절액중추화인자CCR5、CXCL10적영향.방법 선취Wista대서36지,안수궤수자표법분위공백대조조、모형조、추수선감조、백려호순저제량조、백려호순중제량조、백려호순고제량조,각조상응채용생리염수、추수선감、백려호순저、중、고제량관위7d(1차/d),모형조급각실험조우관위제4천장25 mg/ml(0.05 ml)농도적뇨산염용액주사도대서과관절강내,제비급성통풍성관절염모형,공백대조조대서관절강내주사생리염수0.05 ml,분별우조모후2、9、24、48、72 h취혈,72 h후류취과관절관절액,관찰각조불동시간점관절종창지수적변화급혈청관절액중백세포개소(IL)-1β、CCR5、CXCL10적변화.결과 관절액중,저、중、고제량조IL-1β함량분별위(9.63±0.71)、(7.67±0.48)화(6.66 ±0.29) pg/ml,여모형조(11.85±0.45) pg/ml비교명현하강(P＜0.05),중、고제량조CCR5함량분별위(4.49±0.25)화(4.65±0.25) ng/ml,여모형조(5.64±0.28) ng/ml비교명현하강(P＜0.05),중고제량조CXCL10함량분별위(86.79±2.90)화(89.61±5.47) pg/ml,여모형조(110.13±4.02) pg/ml비교명현하강(P＜0.05),병리결과현시,백려호순가감경급성통풍성관절염대서과관절조직적수종화염성세포침윤.결론 급성통풍성관절염발병과정중IL-1β、추화인자CCR5、CXCL10명현증고,백려호순가유효억제급성통풍성관절염발작,차해작용정일정적제량의뢰성.
[Abstract] Objective To study the effects of resveratrol on chemokines CCR5,CXCL10 of serum and synovial fluid in acute gouty arthritis of rats.Methods According to random number table,36 Wista rats were divided into six groups:i.e.control group,model group,colchicine group,high dose resveratrol group (HR),medium dose resveratrol group (MR),and low dose resveratrol group (LR).The rats were respectively administrated with normal saline,colchicine suspension,high dose resveratrol,medium dose resveratrol,low dose resveratrol for 7 days,once a day.Rats acute gouty arthritis modles were estalished on the fourth day by monosodium urate solution (concentration of 25 mg/ml,0.05 ml) injected into the ankle cavity,while 0.05 ml normal saline was injected into joint cavity of rats in control group.2,9,24,48,72 hours after model estabilishment,blood samples were collected from inner canthus to detect levels of interleukin (IL)-1β,CCR5,CXCL10.72 hours after model estabilishment,synovial fluid were collected from ankel to detect levels of IL-1β,CCR5,CXCL10.The joint swelling index was observed periodically.Results The levels of IL-1 β in the low,medium,and high doses of resveratrol were (9.63 ± 0.71),(7.67 ± 0.48),and (6.66 ± 0.29) pg/ml respectively,being significantly lower than that in the model group (11.85 ± 0.45) pg/ml (P＜0.05) in joint fluid.The levels of CCR5 in the medium and high doses of resveratrol groups were(4.49 ± 0.25) and (4.65 ± 0.25) ng/ml respectively,being significantly lower than that in the model group (5.64 ± 0.28) ng/ml (P＜0.05).The levels of CXCL10 in the medium and high doses of resveratrol groups were significantly lower than that in the model group [(86.79 ± 2.90) and (89.61 ± 5.47) vs (110.13 ± 4.02) pg/ml,P＜ 0.05].The histopathologic results showed that resveratrol might reduce acute gouty arthritis joint edema,improve inflammatory cells infiltrating.Conclusions The levels of IL-1β,CCR5,and CXCL10 were raised in acute gouty arthritis.Resveratrol effectively controls acute gouty arthritis attack,and the curative effect is dose dependent.