CAJ | 학술논문

目的探讨JAZF1 (juxtaposed with another zinc finger gene 1)基因对3T3-L1脂肪细胞蛋白激酶B(Akt)和AMP活化的蛋白激酶(AMPK)信号通路的影响.方法构建pGenesil-JAZF1 shRNA重组质粒,转染3T3-L1脂肪细胞后分别用胰岛素或利拉鲁肽处理细胞.2-脱氧-3H-D-葡萄糖掺入法检测细胞的葡萄糖摄取,Western印迹法检测各组细胞Akt和AMPK的磷酸化水平.结果成功构建的pGenesil-JAZF1shRNA重组载体转染3T3-L1脂肪细胞后下调JAZF1表达,使基础和胰岛素刺激的葡萄糖摄取降低33.6％和38.8％(均P＜0.05).胰岛素或利拉鲁肽增加3T3-L1脂肪细胞Akt和AMPK磷酸化水平(均P＜0.05),JAZF1表达下调降低基础和2种激素刺激的Akt和AMPK活性(均P＜0.05).结论 JAZF1基因可能通过Akt和AMPK信号通路改善糖代谢.
목적 탐토JAZF1 (juxtaposed with another zinc finger gene 1)기인대3T3-L1지방세포단백격매B(Akt)화AMP활화적단백격매(AMPK)신호통로적영향.방법 구건pGenesil-JAZF1 shRNA중조질립,전염3T3-L1지방세포후분별용이도소혹리랍로태처리세포.2-탈양-3H-D-포도당참입법검측세포적포도당섭취,Western인적법검측각조세포Akt화AMPK적린산화수평.결과 성공구건적pGenesil-JAZF1shRNA중조재체전염3T3-L1지방세포후하조JAZF1표체,사기출화이도소자격적포도당섭취강저33.6％화38.8％(균P＜0.05).이도소혹리랍로태증가3T3-L1지방세포Akt화AMPK린산화수평(균P＜0.05),JAZF1표체하조강저기출화2충격소자격적Akt화AMPK활성(균P＜0.05).결론 JAZF1기인가능통과Akt화AMPK신호통로개선당대사.
Objective To explore the effect of juxtaposed with another zinc finger gene 1 (JAZF1) on protein kinase B (Akt) and AMP-activated protein kinase (AMPK) signal pathway in 3T3-L1 adipocytes.Methods Recombinant pGenesil-JAZF1shRNA plasmids were constructed.3T3-L1 adipocytes transfected with pGenesil-HK or pGenesil-JAZF1 plasmid were treated with insulin or liraglutide.Glucose uptake was detected by 2-deoxy-3H-glucose.The phosphorylations of Akt and AMPK in 3T3-L1 adipocytes were detected by Western blot.Results Recombinant pGenesil-JAZF1shRNA plasmids was transfected into 3T3-L1 adipocytes and JAZF1 expression was downregulated,resulting in decreased basal and insulin-stimulated glucose uptake by 33.6％ and 38.8％ (both P＜ 0.05).Insulin and liraglutide markedly increased the phosphorylation levels of Akt and AMPK in 3T3-L1 adipocytes (P ＜ 0.05).The downregulation of JAZF1 expression decreased basal and this two hormones-stimauhed phophorylations of Akt and AMPK (all P＜0.05).Conclusion JAZF1 gene may improve glucose metabolism through Akt and AMPK signal pathway.