中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2014年
1期
69-72
,共4页
王吉娇%李春艳%袁晓岚%周姣姣%严静%初蕊%杨丰强%曲伸%苏本利
王吉嬌%李春豔%袁曉嵐%週姣姣%嚴靜%初蕊%楊豐彊%麯伸%囌本利
왕길교%리춘염%원효람%주교교%엄정%초예%양봉강%곡신%소본리
过氧化物酶体增殖物激活受体γ%噻唑烷二酮类%吡格列酮%膀胱癌
過氧化物酶體增殖物激活受體γ%噻唑烷二酮類%吡格列酮%膀胱癌
과양화물매체증식물격활수체γ%새서완이동류%필격렬동%방광암
PPARγ%Thiazolidinediones%Pioglitazone%Bladder cancer
以不同浓度吡格列酮处理膀胱正常移行上皮细胞及膀胱癌J82细胞24 h 、48 h 、72 h、5 d、8 d和10d,观察细胞生长状态,MTT检测细胞生长抑制率,流式细胞仪分析细胞凋亡,实时定量PCR和Western印迹法检测周期素D1 、p53、Bcl-2、Bax的mRNA和蛋白表达.结果显示,10 μmol/L吡格列酮处理膀胱正常细胞24、48、72 h后细胞生长明显受抑制(均P＜0.05),细胞凋亡增加[24 h(18.8 ±2.1对9.4±1.7)％ 、48 h(29.9±1.3对10.7±1.1)、72 h(49.7±2.3对11.3±1.3)％,P＜0.05],J82细胞未见明显影响 . 10 μmol/L吡格列酮处理2种细胞72 h内周期素D1 、p53、Bcl-2、Bax的mRNA和蛋白表达均无明显变化,作用8d后J82细胞周期素D1 、p53蛋白表达降低,提示吡格列酮不增加膀胱正常移行上皮细胞癌变趋势.
以不同濃度吡格列酮處理膀胱正常移行上皮細胞及膀胱癌J82細胞24 h 、48 h 、72 h、5 d、8 d和10d,觀察細胞生長狀態,MTT檢測細胞生長抑製率,流式細胞儀分析細胞凋亡,實時定量PCR和Western印跡法檢測週期素D1 、p53、Bcl-2、Bax的mRNA和蛋白錶達.結果顯示,10 μmol/L吡格列酮處理膀胱正常細胞24、48、72 h後細胞生長明顯受抑製(均P＜0.05),細胞凋亡增加[24 h(18.8 ±2.1對9.4±1.7)％ 、48 h(29.9±1.3對10.7±1.1)、72 h(49.7±2.3對11.3±1.3)％,P＜0.05],J82細胞未見明顯影響 . 10 μmol/L吡格列酮處理2種細胞72 h內週期素D1 、p53、Bcl-2、Bax的mRNA和蛋白錶達均無明顯變化,作用8d後J82細胞週期素D1 、p53蛋白錶達降低,提示吡格列酮不增加膀胱正常移行上皮細胞癌變趨勢.
이불동농도필격렬동처리방광정상이행상피세포급방광암J82세포24 h 、48 h 、72 h、5 d、8 d화10d,관찰세포생장상태,MTT검측세포생장억제솔,류식세포의분석세포조망,실시정량PCR화Western인적법검측주기소D1 、p53、Bcl-2、Bax적mRNA화단백표체.결과현시,10 μmol/L필격렬동처리방광정상세포24、48、72 h후세포생장명현수억제(균P＜0.05),세포조망증가[24 h(18.8 ±2.1대9.4±1.7)％ 、48 h(29.9±1.3대10.7±1.1)、72 h(49.7±2.3대11.3±1.3)％,P＜0.05],J82세포미견명현영향 . 10 μmol/L필격렬동처리2충세포72 h내주기소D1 、p53、Bcl-2、Bax적mRNA화단백표체균무명현변화,작용8d후J82세포주기소D1 、p53단백표체강저,제시필격렬동불증가방광정상이행상피세포암변추세.
The normal bladder transitional cells and bladder cancer J82 cells were treated with various concentrations of pioglitazone for 24 h,48 h,72 h,8 d,and 10 d.The growth of the cells was tested by MTT.Apoptosis rate was detected by flow cytometry technology.Realtime-PCR and Western blot were used to analyze the mRNA and protein expressions of p53,cvclin D1,Bcl-2,and Bax.lhe results showed that the cell viability of normal bladder cells was decreased 24,48,and 72 h after 10 μmol/L pioglitazone treatment (all P＜0.05) while the cell apoptosiswasincreased [24h(18.8±2.1 vs 9.4 ± 1.7) ％,48 h (29.9 ± 1.3 vs 1 0.7 ± 1.1),72 h (49.7 ±2.3 vs11.3 ± 1.3) ％,P＜0.05].Pioglitazone had no effect on the growth and proliferation of J82 cells.There were no significant differences in mRNA and protein expressions of p53,cvclin D1,Bcl-2,and Bax within 72 h after pioglitazone treatment in this two kinds of cells.But,the protein expressions of cvclin D1 and p53 in J82 cells were decreased after pioglitazone treatment for 8 days.These results suggest that pioglitazone does not increase the risk of bladder cancer in normal transitional cells.
