中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2014年
5期
377-380
,共4页
李丽%杨明兰%张娟娟%王柏凯%高捷%叶蕾%洪洁%王卫庆%宁光
李麗%楊明蘭%張娟娟%王柏凱%高捷%葉蕾%洪潔%王衛慶%寧光
리려%양명란%장연연%왕백개%고첩%협뢰%홍길%왕위경%저광
糖尿病,1型%外周血单个核细胞%滤泡辅助性T细胞
糖尿病,1型%外週血單箇覈細胞%濾泡輔助性T細胞
당뇨병,1형%외주혈단개핵세포%려포보조성T세포
Diabetes mellitus,type 1%Peripheral blood mononuclear cell%Follicular helper T cells
目的 观察初发1型糖尿病患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)扣滤泡辅助性T细胞(follicular helperT cells,Tfh)的数目和功能的变化,并与B淋巴细胞的变化及自身抗本等临床资料进行相关性分析,探讨Tfh参与1型糖尿病发病的免疫学机制.方法 收集18例新诊断的1型糖尿病患者和15名健康对照者外周血全血,分离PBMC后流式细胞仪检测CD4+CXCR5+ ICOS+ Tfh及CD19+B淋巴细胞比例,观察二者在1型糖尿病患者中的变化趋势;实时荧光定量PCR检测Tfh细胞因子白细胞介素21(IL-21)及人B细胞淋巴瘤因子6(Bcl-6)的mRNA水平;分析Tfh细胞与抗谷氨酸脱羧酶抗本(GADA)滴度的相关性.结果 (1)与正常对照组相比,新诊断的1型糖尿病患者外周血中的Tfh细胞比例显著上升[(0.09±0.01对0.20±0.01)％,P＜0.01],同时伴有B淋巴细胞比例增多[(1.68±0.16对3.40±0.25)％,P＜0.01];(2)在1型糖尿病患者PBMC中Bcl-6的mRNA表达下调30％ (P＜0.01),IL-21的mRNA水平下降35％(P＜0.05);(3)1型糖尿病患者PBMC中Tfh水平与GADA水平呈显著正相关(r=0.792,P＜0.01).结论 初发1型糖尿病患者外周血Tfh比例显著增加,伴随B淋巴细胞及GADA滴度的上升,表明Tfh可能参与了1型糖尿病的发病.
目的 觀察初髮1型糖尿病患者外週血單箇覈細胞(peripheral blood mononuclear cell,PBMC)釦濾泡輔助性T細胞(follicular helperT cells,Tfh)的數目和功能的變化,併與B淋巴細胞的變化及自身抗本等臨床資料進行相關性分析,探討Tfh參與1型糖尿病髮病的免疫學機製.方法 收集18例新診斷的1型糖尿病患者和15名健康對照者外週血全血,分離PBMC後流式細胞儀檢測CD4+CXCR5+ ICOS+ Tfh及CD19+B淋巴細胞比例,觀察二者在1型糖尿病患者中的變化趨勢;實時熒光定量PCR檢測Tfh細胞因子白細胞介素21(IL-21)及人B細胞淋巴瘤因子6(Bcl-6)的mRNA水平;分析Tfh細胞與抗穀氨痠脫羧酶抗本(GADA)滴度的相關性.結果 (1)與正常對照組相比,新診斷的1型糖尿病患者外週血中的Tfh細胞比例顯著上升[(0.09±0.01對0.20±0.01)％,P＜0.01],同時伴有B淋巴細胞比例增多[(1.68±0.16對3.40±0.25)％,P＜0.01];(2)在1型糖尿病患者PBMC中Bcl-6的mRNA錶達下調30％ (P＜0.01),IL-21的mRNA水平下降35％(P＜0.05);(3)1型糖尿病患者PBMC中Tfh水平與GADA水平呈顯著正相關(r=0.792,P＜0.01).結論 初髮1型糖尿病患者外週血Tfh比例顯著增加,伴隨B淋巴細胞及GADA滴度的上升,錶明Tfh可能參與瞭1型糖尿病的髮病.
목적 관찰초발1형당뇨병환자외주혈단개핵세포(peripheral blood mononuclear cell,PBMC)구려포보조성T세포(follicular helperT cells,Tfh)적수목화공능적변화,병여B림파세포적변화급자신항본등림상자료진행상관성분석,탐토Tfh삼여1형당뇨병발병적면역학궤제.방법 수집18례신진단적1형당뇨병환자화15명건강대조자외주혈전혈,분리PBMC후류식세포의검측CD4+CXCR5+ ICOS+ Tfh급CD19+B림파세포비례,관찰이자재1형당뇨병환자중적변화추세;실시형광정량PCR검측Tfh세포인자백세포개소21(IL-21)급인B세포림파류인자6(Bcl-6)적mRNA수평;분석Tfh세포여항곡안산탈최매항본(GADA)적도적상관성.결과 (1)여정상대조조상비,신진단적1형당뇨병환자외주혈중적Tfh세포비례현저상승[(0.09±0.01대0.20±0.01)％,P＜0.01],동시반유B림파세포비례증다[(1.68±0.16대3.40±0.25)％,P＜0.01];(2)재1형당뇨병환자PBMC중Bcl-6적mRNA표체하조30％ (P＜0.01),IL-21적mRNA수평하강35％(P＜0.05);(3)1형당뇨병환자PBMC중Tfh수평여GADA수평정현저정상관(r=0.792,P＜0.01).결론 초발1형당뇨병환자외주혈Tfh비례현저증가,반수B림파세포급GADA적도적상승,표명Tfh가능삼여료1형당뇨병적발병.
Objective To observe the changes in follicular helper T cells (Tfh) in peripheral blood mononuclear cell (PBMC) of newly-diagnosed patients with type 1 diabetes mellitus (T1DM),to analyze its relationship with B lymphocyte and autoantibodies,and to explore the role played by Tfh cells in the pathogenesis of T1DM.Methods Eighteen patients with newly-diagnosed T1DM and 15 healthy control subjects were enrolled.The percentages of Tfh cells and B cells in PBMC with respective CD4+CXCRS+ICOS+and CD19+markers were assessed by flowcytometry.The mRNA expressions of interluekin 21 (IL-21) and Bcl-6 were detected in two groups by realtime PCR,and the association between Tfh and glutamic acid decarboxylase antibody (GADA) was analyzed.Results (1) The proportions of Tfh [(0.09 ± 0.01 vs 0.20 ± 0.01) ％,P＜0.01] and B lymphocyte [(1.68 ± 0.16 vs 3.4 ±0.25)％,P＜0.01] in PBMC from T1DM group were significantly higher than those in control group.(2) In comparison with control group,mRNA levels of Bcl-6 and IL-21 in PBMC of T1DM group were decreased by 30％ (P＜0.01) and 35％ (P＜0.05).(3) A positive correlation between the amount of Tfh cell and the titer of GADA was found (r =0.792,P ＜ 0.01).Conclusion The close relationship of Tfh cells with clinical and biochemical characteristics in newly-diagnosed patients with T1DM demonstrates an important role which may be played by Tfh cells in the pathogenesis of T1DM.
