CAJ | 학술논문

目的比较2型糖尿病大鼠和正常大鼠在急性败血症合并应激性高血糖后的炎症反应程度和氧化应激的损伤情况.方法将26周龄2型糖尿病GK大鼠和普通Wistar大鼠腹腔注射5 mg/kg脂多糖构建大鼠急性败血症模型.比较脂多糖注射前后2种大鼠血清中促炎因子肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β、IL-6和抗炎因子IL-10水平;化学比色法测定各组大鼠血清和脏器组织(肺脏、肝脏、肾脏、心脏)丙二醛、总抗氧化能力(T-AOC)水平.结果在基线水平,GK大鼠的促炎因子水平(TNF-α、IL-6、IL-10)高于普通大鼠,注射脂多糖后,GK大鼠促炎因子(TNF-α、IL-6)上升幅度明显小于Wistar大鼠(P＜0.05),而抗炎因子(IL-10)上升幅度则明显高于Wistar大鼠;在基线水平,GK大鼠血清的丙二醛水平显著高于Wistar大鼠[(25.76对12.71)μmoL/L,P＜0.05],而血清T-AOC水平则明显低于Wistar大鼠[(0.60对2.8)U/ml,P＜0.05],脂多糖注射1h后2种大鼠血清丙二醛含量较注射前显著升高[(32.30和20.19) μmol/L],T-AOC水平[(0.20和2.08)U/ml]均较基线水平明显下降(P＜0.05),且2种大鼠的变化幅度没有明显差异(P＞0.05),2种大鼠各脏器组织(肺脏、肝脏、肾脏、心脏)的丙二醛、T-AOC水平在脂多糖注射前后差异均无统计学意义(P＞0.05).结论 2型糖尿病大鼠与普通大鼠相比,在急性败血症状态下促炎因子释放减少,抗炎因子释放增加,对急性炎症反应耐受性更高,引起炎症失控性扩散的可能性更小.
목적 비교2형당뇨병대서화정상대서재급성패혈증합병응격성고혈당후적염증반응정도화양화응격적손상정황.방법 장26주령2형당뇨병GK대서화보통Wistar대서복강주사5 mg/kg지다당구건대서급성패혈증모형.비교지다당주사전후2충대서혈청중촉염인자종류배사인자α(TNF-α)、백세포개소(IL)-1β、IL-6화항염인자IL-10수평;화학비색법측정각조대서혈청화장기조직(폐장、간장、신장、심장)병이철、총항양화능력(T-AOC)수평.결과 재기선수평,GK대서적촉염인자수평(TNF-α、IL-6、IL-10)고우보통대서,주사지다당후,GK대서촉염인자(TNF-α、IL-6)상승폭도명현소우Wistar대서(P＜0.05),이항염인자(IL-10)상승폭도칙명현고우Wistar대서;재기선수평,GK대서혈청적병이철수평현저고우Wistar대서[(25.76대12.71)μmoL/L,P＜0.05],이혈청T-AOC수평칙명현저우Wistar대서[(0.60대2.8)U/ml,P＜0.05],지다당주사1h후2충대서혈청병이철함량교주사전현저승고[(32.30화20.19) μmol/L],T-AOC수평[(0.20화2.08)U/ml]균교기선수평명현하강(P＜0.05),차2충대서적변화폭도몰유명현차이(P＞0.05),2충대서각장기조직(폐장、간장、신장、심장)적병이철、T-AOC수평재지다당주사전후차이균무통계학의의(P＞0.05).결론 2형당뇨병대서여보통대서상비,재급성패혈증상태하촉염인자석방감소,항염인자석방증가,대급성염증반응내수성경고,인기염증실공성확산적가능성경소.
Objective To compare the differences in responsive ability and oxidative stress damage between type 2 diabetic rats and normal rats under acute sepsis associated with stress hyperglycemia.Methods GK rats with type 2 diabetes and Wistar rats were established critical ill models of sepsis by intraperitoneal injection of 5 mg/kg lipopolysaccharide(LPS).Before and after LPS injection,serum levels of proinflammatory cytokines tumor necrosis factor α (TNF-α),interleukin (IL)-1β3,and IL-6,and anti-inflammatory cytokine IL-10 were determined.Malondialdehyde (MDA) and total antioxidative capacity (T-AOC) levels in serum and tissues (lung,liver,kidney,heart) were measured by colorimetric determination.Results Before LPS injection,serum levels of TNF-α,IL-6,and IL-10 in GK rats were higher than those of Wistar rats.The serum levels of TNF-α,IL-6,and IL-10 after LPS injection were raised in both GK rats and Wistar rats (all P＜0.05).At baseline,serum MDA level of GK rats was higher than that of Wistar group [(25.76 vs 12.71) μmol/L,P＜0.05],while T-AOC level were lower [(0.60 vs 2.8) U/ml,P＜0.05].One hour after LPS injection,the serum MDA level in two kinds of rats significantly increased compared with those of their baseline levels [(32.30 and 20.19) μmol/L,both P＜0.05],while their serum T-AOC level decreased [(0.20 and 2.08) U/ml,P＜0.05]).There were no signigicant differences in the change trend of serum MDA and T-AOC between the two kinds of rats.MDA and T-AOC levels in tissues had no significant difference before and after LPS injection in GK and Wistar rats (P＞0.05).Conclusions The rats with type 2 diabetes had lowered level of proinflammatory factors and raised level of anti-inflammatory factors under acute sepsis,presenting better tolerance and lowered probability of inflammatory diffusion compared with normal rats.