CAJ | 학술논문

目的探讨γ-干扰素(interferon-γ,IFN-γ)对甲状腺细胞内吲哚胺2,3-双加氧酶(IDO)和色氨酰-tRNA合成酶(TTS)表达的影响及其在Graves病中的免疫病理意义.方法采用实时荧光定量PCR技术,检测经IFN-γ刺激后甲状腺细胞系Nthy-ori3-1和人甲状腺原代细胞内IDO和TTS mRNA表达情况,及甲状腺组织内IDO和TTS mRNA表达.结果人甲状腺细胞系Nthy-ori3-1和人甲状腺原代细胞均可低表达IDO和TTS,经IFN-γ刺激后IDO和TTS mRNA量显著增加(P＜0.01);Graves病患者甲状腺组织高表达TTS,较对照组有统计学差异(P=0.018 2),IDO mRNA水平与对照组相比无明显统计学差异,但与IFN-y mRNA水平呈显著相关性(R2=0.716,P=0.002).结论 Graves病发病早期,甲状腺细胞可能通过IFN-y上调IDO,TTS的表达,降解局部色氨酸,抑制自身反应性淋巴细胞功能,平衡过度的自身免疫反应.
목적 탐토γ-간우소(interferon-γ,IFN-γ)대갑상선세포내신타알2,3-쌍가양매(IDO)화색안선-tRNA합성매(TTS)표체적영향급기재Graves병중적면역병리의의.방법 채용실시형광정량PCR기술,검측경IFN-γ자격후갑상선세포계Nthy-ori3-1화인갑상선원대세포내IDO화TTS mRNA표체정황,급갑상선조직내IDO화TTS mRNA표체.결과 인갑상선세포계Nthy-ori3-1화인갑상선원대세포균가저표체IDO화TTS,경IFN-γ자격후IDO화TTS mRNA량현저증가(P＜0.01);Graves병환자갑상선조직고표체TTS,교대조조유통계학차이(P=0.018 2),IDO mRNA수평여대조조상비무명현통계학차이,단여IFN-y mRNA수평정현저상관성(R2=0.716,P=0.002).결론 Graves병발병조기,갑상선세포가능통과IFN-y상조IDO,TTS적표체,강해국부색안산,억제자신반응성림파세포공능,평형과도적자신면역반응.
Objective To investigate the effect of interferon-γ(IFN-γ) on the expression of indoleamine 2,3-dioxygenase(IDO),and tryptophanyl-tRNA-synthetase (TTS) in thyrocytes; and to study the relevant immunopathological significance in Graves' disease.Methods The expressions of IDO and TTS genes in IFN-γ stimulated Nthy-ori3-1 cell line and human thyrocytes,as well as in human thyroid tissues were determined by realtime quantitative PCR.Results IDO and TTS genes were expressed slightly in both Nthy-ori3-1 cell line and human thyrocytes,and were significantly up-regulated after IFN-γ stimulation(P＜0.01).Compared to healthy controls,TTS mRNA level was higher in thyroid tissues of patients with Graves' disease (P =0.018 2),while IDO mRNA level showed no difference,but was notably correlated with IFN-γ mRNA level (R2 =0.716,P =0.002).Conclusion In the early stage of Graves' disease,thyrocytes may decompose local tryptophan by enhancing the expression of IDO and TTS under IFN-γstimulation,thus inhibit auto-reactive function of lymphocytes and balance excessive autoimmune reaction.