中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2014年
6期
516-519
,共4页
薛君力%邓浩华%曾姣娥%陈小奇%代喆%邹润梅%孙家忠%徐焱成
薛君力%鄧浩華%曾姣娥%陳小奇%代喆%鄒潤梅%孫傢忠%徐焱成
설군력%산호화%증교아%진소기%대철%추윤매%손가충%서염성
受体相互作用蛋白140%过氧化氢%MIN6细胞%增殖%细胞凋亡
受體相互作用蛋白140%過氧化氫%MIN6細胞%增殖%細胞凋亡
수체상호작용단백140%과양화경%MIN6세포%증식%세포조망
Receptor interaction protein 140%Hydrogen peroxide%MIN6 cells%Proliferation%Apoptosis
MIN6细胞给予400 μmol/L H2O2处理分为正常对照组、正常加H2 O2组、Scramble siRNA(Small interfering RNA)组和RIP140 siRNA组,400 μmol/L H2O2能增加MIN6细胞的凋亡率(27.89％±1.91％对3.74％±0.55％,P＜0.01)和丙二醛含量[(35.17±6.26对20.07±1.86) nmol/mg,P＜0.05],明显减少MIN6细胞活力(24和48 h,均P＜0.01)、总超氧化合物歧化酶水平[SOD,(4.79±0.85对3.09±0.60) U/mg,P＜0.05].下调受体相互作用蛋白140(RIP140)表达能减少H2O2诱导的MIN6细胞凋亡率(11.45％ ±2.27％对30.39％±5.32％,P＜0.01)和丙二醛含量[(22.32±1.94对33.42±2.51)nmol/mg,P＜0.01],增加总SOD水平[(5.32±0.40对3.09 ±0.15)nmol/mg,P＜0.01)].
MIN6細胞給予400 μmol/L H2O2處理分為正常對照組、正常加H2 O2組、Scramble siRNA(Small interfering RNA)組和RIP140 siRNA組,400 μmol/L H2O2能增加MIN6細胞的凋亡率(27.89％±1.91％對3.74％±0.55％,P＜0.01)和丙二醛含量[(35.17±6.26對20.07±1.86) nmol/mg,P＜0.05],明顯減少MIN6細胞活力(24和48 h,均P＜0.01)、總超氧化閤物歧化酶水平[SOD,(4.79±0.85對3.09±0.60) U/mg,P＜0.05].下調受體相互作用蛋白140(RIP140)錶達能減少H2O2誘導的MIN6細胞凋亡率(11.45％ ±2.27％對30.39％±5.32％,P＜0.01)和丙二醛含量[(22.32±1.94對33.42±2.51)nmol/mg,P＜0.01],增加總SOD水平[(5.32±0.40對3.09 ±0.15)nmol/mg,P＜0.01)].
MIN6세포급여400 μmol/L H2O2처리분위정상대조조、정상가H2 O2조、Scramble siRNA(Small interfering RNA)조화RIP140 siRNA조,400 μmol/L H2O2능증가MIN6세포적조망솔(27.89％±1.91％대3.74％±0.55％,P＜0.01)화병이철함량[(35.17±6.26대20.07±1.86) nmol/mg,P＜0.05],명현감소MIN6세포활력(24화48 h,균P＜0.01)、총초양화합물기화매수평[SOD,(4.79±0.85대3.09±0.60) U/mg,P＜0.05].하조수체상호작용단백140(RIP140)표체능감소H2O2유도적MIN6세포조망솔(11.45％ ±2.27％대30.39％±5.32％,P＜0.01)화병이철함량[(22.32±1.94대33.42±2.51)nmol/mg,P＜0.01],증가총SOD수평[(5.32±0.40대3.09 ±0.15)nmol/mg,P＜0.01)].
MIN6 cells cultured with 400 μmol/L H2O2 were divided into normal control group,control group with 400 μmol/L H2O2,scrambled siRNA group,and RIP140 siRNA group.400 μmol/L H2O2 could significantly increase apoptosis rate of MIN6 cells(27.89％ ± 1.91％ vs 3.74％ ±0.55％,P＜0.01) and the content of maleic dialdehyde [M DA,(35.17 ± 6.26 vs 20.07 ± 1.86) nmol/mg,P＜0.05],suppress cell viability (24 and 48 h,both P＜0.01),and lower the level of total superoxide dismutase [SOD,(4.79 ± 0.85 vs 3.09 ± 0.60) U/mg,P＜ 0.05].When receptor interaction protein 140 (RIP140) expression was suppressed by RNA interference,the apoptosis rate of MIN6 cells (11.45 ％ ± 2.27％ vs 30.39％ ± 5.32％,P＜0.01) and the content of MDA [(22.32 ± 1.94 vs 33.42 ± 2.51) nmol/mg,P＜0.01] were reduced,and levels of total SOD was increased [(5.32 ± 0.40 vs 3.09±0.15) nmol/mg,P＜0.01)].
