中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2014年
7期
614-616
,共3页
王加峰%蒋健骏%邵远%王根年%陈默%张有成
王加峰%蔣健駿%邵遠%王根年%陳默%張有成
왕가봉%장건준%소원%왕근년%진묵%장유성
分泌型卷曲相关蛋白5%二甲双胍%糖尿病%肝脏%p-JNK
分泌型捲麯相關蛋白5%二甲雙胍%糖尿病%肝髒%p-JNK
분비형권곡상관단백5%이갑쌍고%당뇨병%간장%p-JNK
Secreted frizzled-related protein 5%Metformin%Diabetes%Liver%p-JNK
采用腹腔注射链脲佐菌素(STZ)制备糖尿病大鼠模型,二甲双胍干预5周,采用ELISA法检测血浆中分泌型卷曲相关蛋白5(SFRP5)水平,免疫组化法检测肝脏p-JNK.与对照组相比,糖尿病组大鼠胰岛素抵抗及肝脏p-JNK水平升高(P＜0.05或P＜0.01),血浆中SFRP5水平下降(P＜0.01).经二甲双胍治疗后大鼠胰岛素抵抗明显改善,肝脏p-JNK水平下降(P＜0.05或P＜0.01),SFRP5水平升高(P＜0.01).二甲双胍可能通过上调糖尿病大鼠血浆SFRP5水平增加胰岛素敏感性.
採用腹腔註射鏈脲佐菌素(STZ)製備糖尿病大鼠模型,二甲雙胍榦預5週,採用ELISA法檢測血漿中分泌型捲麯相關蛋白5(SFRP5)水平,免疫組化法檢測肝髒p-JNK.與對照組相比,糖尿病組大鼠胰島素牴抗及肝髒p-JNK水平升高(P＜0.05或P＜0.01),血漿中SFRP5水平下降(P＜0.01).經二甲雙胍治療後大鼠胰島素牴抗明顯改善,肝髒p-JNK水平下降(P＜0.05或P＜0.01),SFRP5水平升高(P＜0.01).二甲雙胍可能通過上調糖尿病大鼠血漿SFRP5水平增加胰島素敏感性.
채용복강주사련뇨좌균소(STZ)제비당뇨병대서모형,이갑쌍고간예5주,채용ELISA법검측혈장중분비형권곡상관단백5(SFRP5)수평,면역조화법검측간장p-JNK.여대조조상비,당뇨병조대서이도소저항급간장p-JNK수평승고(P＜0.05혹P＜0.01),혈장중SFRP5수평하강(P＜0.01).경이갑쌍고치료후대서이도소저항명현개선,간장p-JNK수평하강(P＜0.05혹P＜0.01),SFRP5수평승고(P＜0.01).이갑쌍고가능통과상조당뇨병대서혈장SFRP5수평증가이도소민감성.
Diabetic rat model accompanied by insulin resistance was established by intraperitoneal injection of streptozocin.Following metformin treatment for 5 weeks,ELISA was used to detect the level of plasma secreted frizzled-related protein (SFRP) 5,and immunohistochemistry was used to detect the expression of p-JNK in the liver.insulin resistance(IR) and p-JNK were significantly increased in diabetic group compared with the control group (P＜0.05),while plasma SFRP5 level was reduced (P＜0.01).After metformin treatment,the plasma SFRP5 levels were significantly increased (P＜0.05),while IR and p-JNK was decreased (P＜O.05).Metformin may ameliorate insulin resistance via upregulating the SFRP5 expression of diabetic rats.
