中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2014年
10期
830-833
,共4页
俞灵莺%马丽珍%屠巧峰%陈岳明%张祎%方建华
俞靈鶯%馬麗珍%屠巧峰%陳嶽明%張祎%方建華
유령앵%마려진%도교봉%진악명%장의%방건화
甲状腺乳头状癌%RET/PTC重排%细针抽吸%聚合酶链反应
甲狀腺乳頭狀癌%RET/PTC重排%細針抽吸%聚閤酶鏈反應
갑상선유두상암%RET/PTC중배%세침추흡%취합매련반응
Papillary thyroid carcinoma%RET/PTC rearrangement%Fine needle aspiration%Polymerase chain reaction
目的 探讨本地区甲状腺结节针吸细胞甲状腺乳头状癌的原癌基因(RET/PTC)1和RET/PTC3重排的临床意义.方法 选择2012年1月至2013年1月间甲状腺结节针吸细胞(FNA)标本285份,用荧光定量PCR(FQ-PCR)法检测RET/PTC1、3重排.结果 甲状腺结节RET/PTC1、3重排率分别为17.2％(49/285),1.4％(4/285).平均随访21.7个月,19例(40.4％,19/47) RET/PTC1阳性患者术后病理证实为甲状腺乳头状癌(PTC);RET/PTC1阳性患者中Thy5和Thy4组患者均为PTC,Thy2组进展为PTC较野生型者多(22.6％对3.2％,x2=6.667,P＜0.01));另有4例(8.5％,4/47) RET/PTC1重排阳性为甲状腺良性病变.结论 用FQ-PCR检测FNA标本RET/PTC1、3重排简便、可行;RET/PTC1阳性常见于PTC,甲状腺良性病变也有少量检出.
目的 探討本地區甲狀腺結節針吸細胞甲狀腺乳頭狀癌的原癌基因(RET/PTC)1和RET/PTC3重排的臨床意義.方法 選擇2012年1月至2013年1月間甲狀腺結節針吸細胞(FNA)標本285份,用熒光定量PCR(FQ-PCR)法檢測RET/PTC1、3重排.結果 甲狀腺結節RET/PTC1、3重排率分彆為17.2％(49/285),1.4％(4/285).平均隨訪21.7箇月,19例(40.4％,19/47) RET/PTC1暘性患者術後病理證實為甲狀腺乳頭狀癌(PTC);RET/PTC1暘性患者中Thy5和Thy4組患者均為PTC,Thy2組進展為PTC較野生型者多(22.6％對3.2％,x2=6.667,P＜0.01));另有4例(8.5％,4/47) RET/PTC1重排暘性為甲狀腺良性病變.結論 用FQ-PCR檢測FNA標本RET/PTC1、3重排簡便、可行;RET/PTC1暘性常見于PTC,甲狀腺良性病變也有少量檢齣.
목적 탐토본지구갑상선결절침흡세포갑상선유두상암적원암기인(RET/PTC)1화RET/PTC3중배적림상의의.방법 선택2012년1월지2013년1월간갑상선결절침흡세포(FNA)표본285빈,용형광정량PCR(FQ-PCR)법검측RET/PTC1、3중배.결과 갑상선결절RET/PTC1、3중배솔분별위17.2％(49/285),1.4％(4/285).평균수방21.7개월,19례(40.4％,19/47) RET/PTC1양성환자술후병리증실위갑상선유두상암(PTC);RET/PTC1양성환자중Thy5화Thy4조환자균위PTC,Thy2조진전위PTC교야생형자다(22.6％대3.2％,x2=6.667,P＜0.01));령유4례(8.5％,4/47) RET/PTC1중배양성위갑상선량성병변.결론 용FQ-PCR검측FNA표본RET/PTC1、3중배간편、가행;RET/PTC1양성상견우PTC,갑상선량성병변야유소량검출.
Objective To evaluate the clinical significance of rearrangements during transfection of papillary thyroid cancer (RET/PTC) 1,3 in fine needle aspiration (FNA) specimen from regional thyroid nodules by FQ-PCR.Methods Two hundred and eighty-five FNA samples were collected from patients with thyroid nodules during January 2012 to January 2013.RET/PTC1,3 rearrangements were detected with FQ-PCR.Results The frequencies of the RET/PTC1 and RET/PTC3 rearrangements in 285 FNA samples were 17.2％ (49/285) and 1.4％ (4/285),respectively.During 21.7 months of follow-up,19 (40.4％,19/47) RET/PTC1 positive patients were confirmed to have papillary thyroid carcinoma(PTC) after operation.In the patients with RET/PTC1 rearrangement PTC was found in Thy5 and Thy4 groups.In Thy 2 group,22.6％ cases with RET/PTC1 rearrangements developed PTC as compared with 3.2％ cases without it(x2 =6.667,P＜0.01).In addition,8.5％ (4/47) RET/PTC1 rearrangements emerged in benign lesions.Conclusions It is convenient and reliable to detect RET/PTC1,3 rearrangements by FQ-PCR using FNA samples.RET/PTC1 rearrangement frequently occurs in PTC,however it would be detected in benign lesions occasionally.
