CAJ | 학술논문

目的研究体外模拟细胞内活性氧(reactive oxygen species,ROS)抑制对晚期糖基化终末产物(advanced glycation end products,AGEs)引起的小鼠3T3-L1脂肪细胞脂联素(adiponectin,ADPN)表达水平的影响.方法制备AGEs,体外培养3T3-L1前脂肪细胞,并将其诱导分化为成熟脂肪细胞,油红O染色鉴定脂肪细胞分化程度和脂质积聚情况.予以AGEs刺激脂肪细胞不同的时间,以活性氧(ROS)捕获剂双氢-乙酰乙酸二氯荧光黄(DCFH-DA)孵育细胞,通过流式细胞仪检测细胞内二氯荧光黄(DCF)的荧光强度而测得细胞内ROS水平,采用实时荧光定量PCR与酶联免疫吸附法检测脂联素的mRNA和蛋白水平;予以抗氧化剂N-乙酰-半胱氨酸(N-Acetylcysteine,NAC)预处理后,再次检测脂联素的mRNA和蛋白水平.结果 AGEs处理3T3-L1脂肪细胞后,细胞内ROS水平明显升高,脂联素mRNA及蛋白水平均显著下调;将ROS抑制后,脂联素的mRNA和蛋白水平均有不同程度回升.结论 AGEs诱导3T3-L1脂肪细胞ROS生成增高,从而抑制了脂联素的表达,参与了胰岛素抵抗的病理生理过程.
목적 연구체외모의세포내활성양(reactive oxygen species,ROS)억제대만기당기화종말산물(advanced glycation end products,AGEs)인기적소서3T3-L1지방세포지련소(adiponectin,ADPN)표체수평적영향.방법 제비AGEs,체외배양3T3-L1전지방세포,병장기유도분화위성숙지방세포,유홍O염색감정지방세포분화정도화지질적취정황.여이AGEs자격지방세포불동적시간,이활성양(ROS)포획제쌍경-을선을산이록형광황(DCFH-DA)부육세포,통과류식세포의검측세포내이록형광황(DCF)적형광강도이측득세포내ROS수평,채용실시형광정량PCR여매련면역흡부법검측지련소적mRNA화단백수평;여이항양화제N-을선-반광안산(N-Acetylcysteine,NAC)예처리후,재차검측지련소적mRNA화단백수평.결과 AGEs처리3T3-L1지방세포후,세포내ROS수평명현승고,지련소mRNA급단백수평균현저하조;장ROS억제후,지련소적mRNA화단백수평균유불동정도회승.결론 AGEs유도3T3-L1지방세포ROS생성증고,종이억제료지련소적표체,삼여료이도소저항적병리생리과정.
Objective To investigate the effects of reactive oxygen species (ROS) inhibition on the down-regulation of adiponectin (ADPN) in mouse 3T3-L1 adipocytes by advanced glycation end-products (AGEs).Methods AGEs were prepared for incubating with cell.3T3-L1 preadipocytes were cultured in vitro and differentiated into mature adipocytes.Cell differentiation and lipid accumulation were determined by oil red O staining.After being intervened with AGEs,2',7'-dichlorofluorescein diacetate (DCFH-DA) was used as a reactive oxygen species (ROS) capture agent and the fluorescent intensity of 2',7 '-dichlorofluorescein (DCF) was detected by flow cytometry.Adiponectin expression under AGEs in 3T3-L1 adipocytes pretreated with N-acetyl-L-cysteine(NAC) or not was detected by real-time fluorescent PCR and ELISA.Results The level of ROS in 3T3-L1 adipocytes treated with AGEs was increased.mRNA and protein of ADPN were down-regulated.After inhibition with ROS,mRNA and protein expressions of ADPN injured by AGEs were ameliorated.Conclusion Exposure of 3T3-L1 adipocytes to AGEs induces oxidative stress in vitro,which decreases the expression of ADPN,and causes functional impairment of adipose cells and insulin resistance.