中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
1期
74-75
,共2页
杨玉宝%葛风晓%阚金庆%高忠礼%李林
楊玉寶%葛風曉%闞金慶%高忠禮%李林
양옥보%갈풍효%감금경%고충례%리림
补肾壮骨合剂%转化生长因子β%血管内皮生长因子类
補腎壯骨閤劑%轉化生長因子β%血管內皮生長因子類
보신장골합제%전화생장인자β%혈관내피생장인자류
Setting broken bone mixture%Transforming growth factor beta%Vascular endothelial growth factors
目的 探讨血管内皮生长因子(VEGF)与转化生长因子β(TGF-β)基因在用“补肾壮骨合剂”治疗骨缺损过程中的表达及影响.方法 普通级健康家兔48只,其中36只完全随机分成实验组和对照组,各18只,均在无菌条件下取右侧桡骨中下1/3用骨磨钻制成15 mm的骨缺损模型；另外12只家兔处死后,取髂骨制成颗粒状骨,植入以上骨缺损处.实验组用“补肾壮骨合剂”复合饲料喂养.对照组用普通饲料喂养.分别于术后3d和1、2、4、6、8周处死实验组与对照组家兔各3只,取骨缺损处标本,应用聚合酶链反应技术检测VEGF和TGF-β基因的表达.结果 VEGF和TGF-β的目的基因,在校正结果后,以对照组术后1周时测得的基因拷贝值为基础,其他各组与其比值:对照组VEGF的值分别为2.08、1.00、0.88、3.40、1.71、1.17,实验组VEGF的值分别为6.50、2.25、1.86、3.13、3.33、3.31;对照组TGF-β的值分别为0.57、1.00、1.40、1.44、1.59、1.27,实验组TGF-β的值分别为0.58、2.05、2.35、2.59、3.18、2.67.实验组和对照组VEGF基因校正值分别为3.3±1.4比1.7±0.9,差异有统计学意义(t=-18.95,P＜0.05),实验组和对照组TGF-β基因校正值分别为2.2±0.9比1.2±0.4,差异有统计学意义(t=-2.594,P＜0.05).结论 补肾壮骨合剂能促进VEGF与TGF-β基因的表达.
目的 探討血管內皮生長因子(VEGF)與轉化生長因子β(TGF-β)基因在用“補腎壯骨閤劑”治療骨缺損過程中的錶達及影響.方法 普通級健康傢兔48隻,其中36隻完全隨機分成實驗組和對照組,各18隻,均在無菌條件下取右側橈骨中下1/3用骨磨鑽製成15 mm的骨缺損模型；另外12隻傢兔處死後,取髂骨製成顆粒狀骨,植入以上骨缺損處.實驗組用“補腎壯骨閤劑”複閤飼料餵養.對照組用普通飼料餵養.分彆于術後3d和1、2、4、6、8週處死實驗組與對照組傢兔各3隻,取骨缺損處標本,應用聚閤酶鏈反應技術檢測VEGF和TGF-β基因的錶達.結果 VEGF和TGF-β的目的基因,在校正結果後,以對照組術後1週時測得的基因拷貝值為基礎,其他各組與其比值:對照組VEGF的值分彆為2.08、1.00、0.88、3.40、1.71、1.17,實驗組VEGF的值分彆為6.50、2.25、1.86、3.13、3.33、3.31;對照組TGF-β的值分彆為0.57、1.00、1.40、1.44、1.59、1.27,實驗組TGF-β的值分彆為0.58、2.05、2.35、2.59、3.18、2.67.實驗組和對照組VEGF基因校正值分彆為3.3±1.4比1.7±0.9,差異有統計學意義(t=-18.95,P＜0.05),實驗組和對照組TGF-β基因校正值分彆為2.2±0.9比1.2±0.4,差異有統計學意義(t=-2.594,P＜0.05).結論 補腎壯骨閤劑能促進VEGF與TGF-β基因的錶達.
목적 탐토혈관내피생장인자(VEGF)여전화생장인자β(TGF-β)기인재용“보신장골합제”치료골결손과정중적표체급영향.방법 보통급건강가토48지,기중36지완전수궤분성실험조화대조조,각18지,균재무균조건하취우측뇨골중하1/3용골마찬제성15 mm적골결손모형；령외12지가토처사후,취가골제성과립상골,식입이상골결손처.실험조용“보신장골합제”복합사료위양.대조조용보통사료위양.분별우술후3d화1、2、4、6、8주처사실험조여대조조가토각3지,취골결손처표본,응용취합매련반응기술검측VEGF화TGF-β기인적표체.결과 VEGF화TGF-β적목적기인,재교정결과후,이대조조술후1주시측득적기인고패치위기출,기타각조여기비치:대조조VEGF적치분별위2.08、1.00、0.88、3.40、1.71、1.17,실험조VEGF적치분별위6.50、2.25、1.86、3.13、3.33、3.31;대조조TGF-β적치분별위0.57、1.00、1.40、1.44、1.59、1.27,실험조TGF-β적치분별위0.58、2.05、2.35、2.59、3.18、2.67.실험조화대조조VEGF기인교정치분별위3.3±1.4비1.7±0.9,차이유통계학의의(t=-18.95,P＜0.05),실험조화대조조TGF-β기인교정치분별위2.2±0.9비1.2±0.4,차이유통계학의의(t=-2.594,P＜0.05).결론 보신장골합제능촉진VEGF여TGF-β기인적표체.
Objective To investigate the expression and influence of vascular endothelial growth factor (VEGF) and transforming growth factor beta(TGF-β) genes during the treatment of bone defect with setting broken bone mixture (nature copper xuduan gusuibu ground beetle).Methods Thirty-six of forty-eight health adult family rabbits were randomly divided into the experimental group and control group.There were 18 adult family rabbits in every group.Right lower 1/3 radius of the experimental group and the control group were made into a 15 mm standard bone defect model with bone grinding machine.Other twelve rabbits were killed to offer homologous ilium spongy.The experimental group was fed with Chinese drug mixture.Three health adult family rabbits were killed at1st,2nd,4th,6th,8th week after operation in the experimental group.Three health adult family rabbits were killed at1st,2nd,4th,6th,8th week after operation in the control group,Real time Polymerase Chain Reaction (RT-PCR) of vascular endothelial growth factor and transforming growth factor beta genes were investigated.Results After VEGF and TGF-β objective genes were revised for the experimental group and the control group; VEGF valve was 2.08,1.00,0.88,3.40,1.71 and 1.17 in the control group; it was 6.50,2.25,1.86,3.13,3.33,3.31 in the experimental group.TGF-β value was 0.57,1.00,1.40,1.44,1.59 and 1.27 in the control group; it was 0.58,2.05,2.35,2.59,3.18 and 2.67 in the experimental group.Adjusted value of VEGF gene was 3.3 ± 1.4 and 1.7 ± 0.9 in the experimental group and the control group (t =-2.594,P ＜ 0.05).Adjusted value of TGF-β gene was 2.2 ± 0.9 and 1.2 ± 0.4 in the experimental group and the control group (t =-2.594,P ＜ 0.05).Conclusion Setting broken bone mixture can promote the expression level of VEGF and TGF-β gene.
