CAJ | 학술논문

目的观察黄芪甲苷对神经干细胞(NSC)体外增殖的影响,为神经干细胞应用于临床提供理论依据.方法利用无血清培养技术,从新生大鼠海马区分离培养NSC,并进行体外扩增、传代.取传至第3代的NSC,观察不同浓度黄芪甲苷(40、80、120 mg/L)对其增殖的影响.对神经球形成数进行计数并进行噻唑蓝(MTT)比色分析.结果 40 mg/L和80 mg/L黄芪甲苷实验组神经球形成数和细胞吸光度值均明显高于对照组(神经球形成数:8.1±1.4,8.8±1.3比7.2±1.2；细胞吸光度值:0.87±0.02、0.89 ±0.03比0.69±0.03,P＜0.05或P＜0.01),而120 mg/L黄芪甲苷组实验组神经球形成数和细胞吸光度值(分别为3.1±1.2、0.30 ±0.02)低于对照组(P＜0.01).结论在一定浓度范围内,黄芪甲苷可促进体外培养NSC的增殖.
목적 관찰황기갑감대신경간세포(NSC)체외증식적영향,위신경간세포응용우림상제공이론의거.방법 이용무혈청배양기술,종신생대서해마구분리배양NSC,병진행체외확증、전대.취전지제3대적NSC,관찰불동농도황기갑감(40、80、120 mg/L)대기증식적영향.대신경구형성수진행계수병진행새서람(MTT)비색분석.결과 40 mg/L화80 mg/L황기갑감실험조신경구형성수화세포흡광도치균명현고우대조조(신경구형성수:8.1±1.4,8.8±1.3비7.2±1.2；세포흡광도치:0.87±0.02、0.89 ±0.03비0.69±0.03,P＜0.05혹P＜0.01),이120 mg/L황기갑감조실험조신경구형성수화세포흡광도치(분별위3.1±1.2、0.30 ±0.02)저우대조조(P＜0.01).결론 재일정농도범위내,황기갑감가촉진체외배양NSC적증식.
Objective To observe the influence of astragaloside on proliferation of neural stem cells (NSCs) in vitro to provide a theoretical basis for clinical application of NSCs.Methods Through serum-free culture technology,NSCs were isolated from the hippocampus of neonatal rats and cultured,and were amplified,passaged.Check spread to the third generation of neural stem cells,the effects of different concentrations of astragaloside (40,80,120 mg/L) for their proliferation were observed.The number of neurospheres formed were counted and MTT colorimetric analysis were conducted.Results The number of neurospheres formed and the optical density (A) in 40 mg/L and 80 mg/L astragaloside group were significantly higher than those in control group (8.1 ±1.4,8.8 ± 1.3 vs 7.2 ±1.2,0.87 ±0.02,0.89 ±0.03 vs 0.69 ±0.03,P ＜0.05 or P ＜0.01),while those in 120 mg/L astragaloside group (3.1 ± 1.2,0.30 ± 0.02,respectively) were significantly lower than those in control group.Conclusion In a certain range of concentration,astragaloside can promote proliferation of neural stem cells in vitro.